Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,820,029 |
A→G |
V381A (GTG→GCG) |
ligB ← |
DNA ligase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,820,029 | 0 | A | G | 100.0%
| 114.2
/ NA
| 32 | V381A (GTG→GCG) | ligB | DNA ligase B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/19); total (13/19) |
CAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCC > NC_000913/3819958‑3820106
|
cAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCAcatcat < 2:742967/81‑1 (MQ=255)
gCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGc < 2:742968/80‑1 (MQ=255)
ggTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAg > 2:743025/1‑80 (MQ=255)
gTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAgg > 2:743013/1‑80 (MQ=255)
aaCGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATa > 1:743011/1‑81 (MQ=255)
gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTg < 1:742969/81‑1 (MQ=255)
ttAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGa > 1:743014/1‑81 (MQ=255)
aGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCgg < 1:742970/81‑1 (MQ=255)
gTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAg < 1:742971/81‑1 (MQ=255)
tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAGTACCCTGACCGGCAAGGCt < 1:742972/80‑1 (MQ=255)
tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCt < 2:742973/80‑1 (MQ=255)
gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa < 1:742974/78‑1 (MQ=255)
gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc < 2:742975/79‑1 (MQ=255)
gtgtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGaa > 1:743032/1‑81 (MQ=255)
ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTg < 2:742976/80‑1 (MQ=255)
ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGa > 1:743039/1‑81 (MQ=255)
ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGa < 2:742977/81‑1 (MQ=255)
gTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGAt > 2:743016/1‑81 (MQ=255)
ttttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATc < 2:742978/81‑1 (MQ=255)
ttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCa < 1:742980/80‑1 (MQ=255)
ttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCa < 2:742979/80‑1 (MQ=255)
tGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCAc < 2:742981/80‑1 (MQ=255)
tACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAg < 1:762548/81‑1 (MQ=255)
gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg < 2:742982/81‑1 (MQ=255)
gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg > 2:743021/1‑81 (MQ=255)
ttCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcgc < 1:742983/81‑1 (MQ=255)
cTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCaa > 2:743036/1‑81 (MQ=255)
cACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAat < 2:742984/79‑1 (MQ=255)
ccGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc > 2:743048/1‑81 (MQ=255)
cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc > 2:743018/1‑80 (MQ=255)
cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc > 2:743037/1‑80 (MQ=255)
gcgcCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCa > 1:743027/1‑81 (MQ=255)
gccacgccacATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACt > 2:743035/1‑81 (MQ=255)
cacgccacaTCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTcc < 2:742986/81‑1 (MQ=255)
cacgccacaTCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTcc < 1:742985/81‑1 (MQ=255)
|
CAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCC > NC_000913/3819958‑3820106
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
