Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,579,647 |
C→A |
G65V (GGC→GTC) |
symE ← |
toxic protein SymE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,579,647 | 0 | C | A | 100.0%
| 107.8
/ NA
| 31 | G65V (GGC→GTC) | symE | toxic protein SymE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/12); total (19/12) |
AGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGA > NC_000913/4579569‑4579722
|
aGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACt > 2:844262/1‑81 (MQ=255)
aGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACt > 1:844250/1‑81 (MQ=255)
gTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACtt < 2:844221/81‑1 (MQ=255)
cacCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCAc > 1:844264/1‑81 (MQ=255)
cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt < 1:844222/81‑1 (MQ=255)
ggCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGa > 1:844267/1‑81 (MQ=255)
aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc > 2:844260/1‑80 (MQ=255)
aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc > 1:844259/1‑80 (MQ=255)
gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc > 1:844261/1‑81 (MQ=255)
gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc > 2:844272/1‑81 (MQ=255)
gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc < 2:844223/81‑1 (MQ=255)
attCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAg > 1:844263/2‑81 (MQ=255)
cGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg < 1:844224/80‑1 (MQ=255)
cGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg < 1:844225/80‑1 (MQ=255)
gCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGc < 2:844226/81‑1 (MQ=255)
ctcGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAAc < 2:844227/80‑1 (MQ=255)
gcggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcg > 2:844274/1‑81 (MQ=255)
cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg > 1:844283/1‑81 (MQ=255)
cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg < 2:844228/81‑1 (MQ=255)
cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg > 1:844257/1‑81 (MQ=255)
gtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCa < 1:844229/81‑1 (MQ=255)
ggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGc > 2:844270/1‑81 (MQ=255)
ttGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCa > 1:844265/1‑81 (MQ=255)
tGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCAc > 2:844268/1‑81 (MQ=255)
gggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACt > 2:844275/1‑81 (MQ=255)
ggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTg < 1:844230/81‑1 (MQ=255)
cGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGAc < 2:844231/81‑1 (MQ=255)
gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt > 1:844280/1‑81 (MQ=255)
aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt < 2:844232/80‑1 (MQ=255)
aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt > 1:844269/1‑80 (MQ=255)
tACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGa > 1:844276/1‑81 (MQ=255)
|
AGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGA > NC_000913/4579569‑4579722
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
