Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,579,647 |
C→A |
G65V (GGC→GTC) |
symE ← |
toxic protein SymE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,579,647 | 0 | C | A | 100.0%
| 113.4
/ NA
| 32 | G65V (GGC→GTC) | symE | toxic protein SymE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/12); total (20/12) |
ACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGT > NC_000913/4579578‑4579720
|
acCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACt > 1:921445/1‑81 (MQ=255)
acCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACt < 2:921386/81‑1 (MQ=255)
cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt > 2:921426/1‑81 (MQ=255)
cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt < 2:921387/81‑1 (MQ=255)
ggCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGa > 1:921423/1‑81 (MQ=255)
gCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGAc < 1:921388/81‑1 (MQ=255)
cGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACa > 2:921429/1‑81 (MQ=255)
tAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc > 2:921444/1‑81 (MQ=255)
aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc > 2:921435/1‑80 (MQ=255)
aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc > 2:921441/1‑80 (MQ=255)
cGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAc < 1:921389/81‑1 (MQ=255)
cTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAccgc > 1:921427/1‑81 (MQ=255)
cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAccgccg < 1:921390/80‑1 (MQ=255)
cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGt > 2:921438/1‑81 (MQ=255)
cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGt < 2:921391/81‑1 (MQ=255)
tCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGc < 2:921392/81‑1 (MQ=255)
tCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg > 2:921448/1‑81 (MQ=255)
tctcCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCa > 2:921419/1‑80 (MQ=255)
gcggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcg > 2:921451/1‑81 (MQ=255)
ggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCggcggc > 2:921442/1‑81 (MQ=255)
gggTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTc > 2:921393/1‑81 (MQ=255)
ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc < 2:921396/81‑1 (MQ=255)
ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc < 1:921395/81‑1 (MQ=255)
ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc < 1:921394/81‑1 (MQ=255)
gtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGc < 2:921397/80‑1 (MQ=255)
ggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACt > 1:921450/1‑80 (MQ=255)
gCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGa > 2:921439/1‑81 (MQ=255)
ggTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGAcc > 2:921398/1‑81 (MQ=255)
gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt > 1:921446/1‑81 (MQ=255)
gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt > 2:921447/1‑81 (MQ=255)
aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt < 2:921399/80‑1 (MQ=255)
aaTACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGt > 1:921452/1‑81 (MQ=255)
|
ACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGT > NC_000913/4579578‑4579720
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
