Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	57	32.8	3585168	30.6%	1097061	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,546	C→G	G360A (GGC→GCC)	uhpC ←	inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,546	0	C	G	100.0%	127.0 / NA	35	G360A (GGC→GCC)	uhpC	inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (20/15); total (20/15)

TATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGT  >  NC_000913/3847409‑3847681

tATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCa                                                                                                                                       >  1:1463437/1‑140 (MQ=255)

   cGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAg                                                                                                                                    >  1:1532388/1‑140 (MQ=255)

                     gCCACCCAGCAGGCGCCCCCCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTg                                                                                                                  <  2:1464150/140‑1 (MQ=255)

                           cagcagGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGaagaag                                                                                                            <  1:1596205/140‑1 (MQ=255)

                              cAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAg                                                                                                         >  2:1538281/1‑140 (MQ=255)

                               aaGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAgg                                                                                                        <  1:1773434/140‑1 (MQ=255)

                                   gccGCCCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  2:1781097/138‑1 (MQ=255)

                                   gACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACCCTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  1:1504175/140‑1 (MQ=255)

                                   gACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  2:1301369/140‑1 (MQ=255)

                                      ccccccAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTg                                                                                                 <  1:1607262/139‑1 (MQ=255)

                                        ccccAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCa                                                                                               >  1:1522788/1‑140 (MQ=255)

                                           cAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCa                                                                                            >  1:1280440/1‑140 (MQ=255)

                                                aaGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAg                                                                                       <  1:1474319/140‑1 (MQ=255)

                                                  gCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCt                                                                                     >  2:1694197/1‑140 (MQ=255)

                                                        aaGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                                               <  2:1532388/140‑1 (MQ=255)

                                                        aaGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                                               >  2:1679088/1‑140 (MQ=255)

                                                            cGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGc                                                                           <  2:1732897/140‑1 (MQ=255)

                                                              ccAAACCCCGTCGCCGCCCCTGCCGCCTCTTGGGGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCAt                                                                         <  2:1441140/139‑1 (MQ=255)

                                                              aCAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCAt                                                                         <  1:1380481/140‑1 (MQ=255)

                                                                     ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcac                                                                  <  1:1694197/140‑1 (MQ=255)

                                                                                             tgtgGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAAtt                                          >  2:1613702/1‑140 (MQ=255)

                                                                                                          tGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGaaaa                             <  2:1463437/140‑1 (MQ=255)

                                                                                                            ccgccgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATc                           >  1:1630152/1‑140 (MQ=255)

                                                                                                              gccgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCaa                         >  2:1522555/1‑140 (MQ=255)

                                                                                                                cgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAAt                       >  2:1762332/1‑140 (MQ=255)

                                                                                                                  ccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGTCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTc                     >  2:1730925/1‑140 (MQ=255)

                                                                                                                  ccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTc                     >  2:1543960/1‑140 (MQ=255)

                                                                                                                    atcccGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCAt                   <  2:1712648/137‑1 (MQ=255)

                                                                                                                      aCCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCg                 >  2:1660743/1‑140 (MQ=255)

                                                                                                                          aTTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGACAATCAAATTCATCGGccc             >  2:1250259/1‑140 (MQ=255)

                                                                                                                            tAACATCTGTGGGGCAAAGACCAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTc           >  1:1511508/1‑140 (MQ=255)

                                                                                                                                 tCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTg      >  2:1682016/1‑140 (MQ=255)

                                                                                                                                 tCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTg      <  1:1538281/140‑1 (MQ=255)

                                                                                                                                    gtgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCg   >  2:1267731/1‑140 (MQ=255)

                                                                                                                                    gtgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCg   >  2:1438218/1‑140 (MQ=255)

                                                                                                                                     tgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGt  >  2:1503167/1‑140 (MQ=255)

TATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGT  >  NC_000913/3847409‑3847681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A