Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F60 I1 R1	18	219.4	7342358	99.0%	7268934	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	98.0%	1093.3 / 8.9	296	pseudogene (151/758 nt)	glpR	DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand): ref base . (2/4); new base G (139/150); total (141/155)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 323 total aligned reads displayed.

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560316‑3560587

gATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑c                                                                                                                                       <  1:2744164/140‑1 (MQ=255)

gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑c                                                                                                                                       >  1:2843184/1‑140 (MQ=255)

 aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑cg                                                                                                                                      <  2:2049214/140‑2 (MQ=255)

 aTAAACAGCGCCGAGCCATGGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑cg                                                                                                                                      <  2:2239679/140‑2 (MQ=255)

   aaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑cgca                                                                                                                                    <  1:2525155/140‑4 (MQ=255)

      cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGa                                                                                                                                 >  1:3536825/1‑140 (MQ=255)

        gCGTCGAGCCATTGGTGATTTGATCAGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATc                                                                                                                               <  1:1399492/140‑1 (MQ=255)

        gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATc                                                                                                                               >  1:1801213/1‑140 (MQ=255)

        gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGAAGCG‑CCGCACCGGGATGATGG‑CGCAGGATc                                                                                                                               >  2:1723120/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTTGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCa                                                                                                                              >  2:613211/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCa                                                                                                                              >  2:838175/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCa                                                                                                                              >  1:3096817/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCa                                                                                                                              >  2:3459266/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTCGAAGGCAGCG‑CCGCACCGCCAGGATGG‑CGCAGGATCa                                                                                                                              >  1:1622640/1‑140 (MQ=255)

         cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGATAACCGAATTGGGAGGGAGCG‑CCGCACCGCAATGATGG‑CGCAGGATCa                                                                                                                              >  2:1137459/1‑140 (MQ=255)

          gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAg                                                                                                                             >  2:3350228/1‑140 (MQ=255)

           tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAgg                                                                                                                            >  1:2281805/1‑140 (MQ=255)

           tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAgg                                                                                                                            >  1:2450684/1‑140 (MQ=255)

                ccttgggggTTTTGCCCCGCCAGTTTGCGGGGGATGCGCACTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTg                                                                                                                       <  2:1591391/135‑1 (MQ=255)

                ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCGCCGACATGATGG‑CTCAGGATCAGGTTTTg                                                                                                                       >  1:1687067/1‑140 (MQ=255)

                         aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                              <  1:3476219/140‑1 (MQ=255)

                         aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                              <  2:639245/140‑1 (MQ=255)

                           ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCG‑CCGCAACGCCACGATGG‑CGCAGGATCACGTTTTGCTACGCCAGCt                                                                                                            >  2:1635922/1‑140 (MQ=255)

                            tGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGc                                                                                                                 <  2:3029251/134‑1 (MQ=255)

                            tGCTCCGACACTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                           >  2:2109700/1‑140 (MQ=255)

                                ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                       >  1:2223150/1‑140 (MQ=255)

                                 cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                              <  2:2815095/132‑1 (MQ=255)

                                 cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                      >  2:2991931/1‑140 (MQ=255)

                                  gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGCTTTGGTCCGCCAGCTCATTGAg                                                                                                     >  2:1270353/1‑140 (MQ=255)

                                   ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg                                                                                                    >  2:2945120/1‑140 (MQ=255)

                                   ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg                                                                                                    >  2:2765477/1‑140 (MQ=255)

                                   ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg                                                                                                    >  2:3182679/1‑140 (MQ=255)

                                       tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                <  1:3412241/140‑1 (MQ=255)

                                        ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTGGCTCCGCCACCTCATTGAGGTcgcg                                                                                               >  1:1367387/1‑140 (MQ=255)

                                           cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACAGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                            >  2:694402/1‑140 (MQ=255)

                                                gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg                                                                                       >  2:3089074/1‑140 (MQ=255)

                                                 aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGATTTGCTCAGCCAGCGCATTGAGGTCGCGGCGAATAGt                                                                                      >  1:1120497/1‑140 (MQ=255)

                                                  tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                     >  1:3057556/1‑140 (MQ=255)

                                                   gcgcTCTTTTTCTTCGGACTGGGTGGCCTTGTGATAGTGTCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                                                                    <  2:1368359/140‑1 (MQ=255)

                                                    cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                   <  2:2208901/140‑1 (MQ=255)

                                                      ctctTTTTCTTCGGTTTGGGTGCCCTTGCTATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                 <  1:1740760/140‑1 (MQ=255)

                                                       tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGACCAGGTTTTGCTCCGCCCGCTCATTGCGGTCCCGGCGAATAGTCTGCgg                                                                                >  2:1338238/1‑140 (MQ=255)

                                                        ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                               >  2:3527857/1‑140 (MQ=255)

                                                              cttcGGTCTGGGTGGGCTTGCGTTCCTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                         <  1:1300999/140‑1 (MQ=255)

                                                               ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                        >  2:670262/1‑140 (MQ=255)

                                                                   gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGTGAATAGTCTGCGGGCTGACGGAGaa                                                                    >  1:1555762/1‑140 (MQ=255)

                                                                     cTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCAGCGTATAGTCTGCCGGCTGACGGAGAAAt                                                                  >  1:1218204/1‑140 (MQ=255)

                                                                            gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                           <  1:2818203/140‑1 (MQ=255)

                                                                             ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                          <  1:577199/140‑1 (MQ=255)

                                                                             ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                          >  2:2354719/1‑140 (MQ=255)

                                                                              cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                         >  1:3218468/1‑140 (MQ=255)

                                                                               ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                        <  2:1922748/140‑1 (MQ=255)

                                                                                  cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGTTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                     >  1:3384344/1‑140 (MQ=255)

                                                                                  cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGATGTCGCGGCAAATAGTCTGCGGGCTGGCGGATAAATGCTCTACCAGctc                                                     >  2:1043875/1‑140 (MQ=255)

                                                                                  cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                     >  2:2843074/1‑140 (MQ=255)

                                                                                  cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                     >  1:296630/1‑140 (MQ=255)

                                                                                       gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                   >  2:2677066/1‑137 (MQ=255)

                                                                                       gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                >  2:3257210/1‑140 (MQ=255)

                                                                                        tGCCACGGCGTGTTAACCGAACGGGAAGGCACCG‑CGGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                               <  2:1025223/140‑1 (MQ=255)

                                                                                         gcccgggCTTGTTAACTCAACGCGAAGGCACCG‑GCACACCGCCATGATTG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                              <  2:1747616/135‑1 (MQ=255)

                                                                                         gCCACGGCGTGTTACTCGAACTTGACGGCAGCG‑CCGCTCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                              <  1:1653515/140‑1 (MQ=255)

                                                                                         gCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                              <  2:2450684/140‑1 (MQ=255)

                                                                                         gCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                              <  1:613211/140‑1 (MQ=255)

                                                                                          ccAGGGCGTGTTACCCGTACTGCAAGGCAGCT‑CCGCACCTCCATGATGT‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  2:1289857/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGATGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  2:3387422/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  2:526012/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  1:3182679/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  1:489818/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  1:880610/140‑1 (MQ=255)

                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  1:756161/140‑1 (MQ=255)

                                                                                           cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             <  1:125502/140‑1 (MQ=255)

                                                                                            aCGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                           >  2:119214/1‑140 (MQ=255)

                                                                                            aCGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGAGCTGACGGAGAGATACTCTACGAGCTCTTCGGTACTg                                           >  1:978513/1‑140 (MQ=255)

                                                                                             cGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                          <  1:2354719/140‑1 (MQ=255)

                                                                                               gCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                        >  2:3287736/1‑140 (MQ=255)

                                                                                               gCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                        <  1:556791/140‑1 (MQ=255)

                                                                                                 gtgtTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGACTGCGGGCCGACCGAGAAATGCTCTCCCAGCTCTTCGGTACTGACATa                                      >  2:1528604/1‑140 (MQ=255)

                                                                                                      aaCCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGGAG‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 <  1:1350538/140‑1 (MQ=255)

                                                                                                      aaCCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 <  1:3459266/140‑1 (MQ=255)

                                                                                                      aaCCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 <  1:380228/140‑1 (MQ=255)

                                                                                                      aaCCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 <  1:1953729/140‑1 (MQ=255)

                                                                                                         cGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                             >  2:572921/1‑125 (MQ=255)

                                                                                                                gAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                       <  2:1243346/140‑1 (MQ=255)

                                                                                                                gAAGGCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                       <  1:119214/140‑1 (MQ=255)

                                                                                                                   ggCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                    <  1:2669105/140‑1 (MQ=255)

                                                                                                                    gCAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                   <  1:3287736/140‑1 (MQ=255)

                                                                                                                     cAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCGGCTATTCGATACTGACATAACCCTGCTGTTTAACCAGtt                  >  2:1679513/1‑140 (MQ=255)

                                                                                                                     cAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAGCCct                                 <  2:2654998/125‑1 (MQ=255)

                                                                                                                     cAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 >  1:2654998/1‑125 (MQ=255)

                                                                                                                     cAGCG‑CCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAACTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                  <  1:651988/140‑1 (MQ=255)

                                                                                                                        cg‑ccgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                    >  1:674637/1‑135 (MQ=255)

                                                                                                                        cg‑ccgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                   <  2:1800410/136‑1 (MQ=255)

                                                                                                                           ccgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata             >  2:3486597/1‑140 (MQ=255)

                                                                                                                            cgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa            <  1:2775487/140‑1 (MQ=255)

                                                                                                                              cACCGCCATAATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata          >  2:484466/1‑140 (MQ=255)

                                                                                                                               aCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc         <  1:299564/140‑1 (MQ=255)

                                                                                                                                ccGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACATATCCCGGCAGTTTAAACAGTTCGATAATAcc        >  1:1030100/1‑140 (MQ=255)

                                                                                                                                   ccATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                 >  2:2485722/1‑128 (MQ=255)

                                                                                                                                     atgatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt   <  1:2075324/140‑1 (MQ=255)

                                                                                                                                      tgatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg  >  2:3605032/1‑140 (MQ=255)

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560316‑3560587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A