Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F48 I1 R1	62	147.6	5031800	99.1%	4986513	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,715	G→T	A24A (GCG→GCT)	murG →	N‑acetylglucosaminyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,715	0	G	T	99.2%	426.2 / ‑3.3	119	A24A (GCG→GCT)	murG	N‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (49/69); total (50/69)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 122 total aligned reads displayed.

ATGCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCGGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTA  >  NC_000913/99579‑99851

atgCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGtt                                                                                                                                       <  1:261488/140‑1 (MQ=255)

  gCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTgc                                                                                                                                     <  2:379056/140‑1 (MQ=255)

  gCTGTTGCGTATTGATTATGAAACGCGACTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTgc                                                                                                                                     >  1:2044845/1‑140 (MQ=255)

   cTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTgcg                                                                                                                                    <  2:687810/140‑1 (MQ=255)

   cTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTgcg                                                                                                                                    <  1:1927662/140‑1 (MQ=255)

   cTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTAGACATGTATTCCCGGGACTGGCTGTTgcg                                                                                                                                    <  1:2225192/140‑1 (MQ=255)

     gTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCa                                                                                                                                  <  2:2275484/140‑1 (MQ=255)

      ttGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCAc                                                                                                                                 >  1:431877/1‑140 (MQ=255)

      ttGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCAc                                                                                                                                 >  2:1044172/1‑140 (MQ=255)

           tATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCt                                                                                                                            >  2:2173012/1‑140 (MQ=255)

           tATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCt                                                                                                                            >  2:242867/1‑140 (MQ=255)

            aTTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTa                                                                                                                           <  2:548151/140‑1 (MQ=255)

              tGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAAt                                                                                                                         >  1:1582556/1‑140 (MQ=255)

                aTTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATgg                                                                                                                       >  1:1007038/1‑140 (MQ=255)

                aTTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATgg                                                                                                                       >  1:2035028/1‑140 (MQ=255)

                 ttATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGc                                                                                                                      <  1:1044172/140‑1 (MQ=255)

                    tGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCa                                                                                                                   <  2:1140492/140‑1 (MQ=255)

                    tGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCa                                                                                                                   <  1:2173012/140‑1 (MQ=255)

                     gAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAg                                                                                                                  >  2:951207/1‑140 (MQ=255)

                      aaaCGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCt                                                                                                                     <  2:662249/136‑1 (MQ=255)

                          gcgTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTg                                                                                                             >  2:411789/1‑140 (MQ=255)

                           cgTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCt                                                                                                                     <  2:1637748/131‑1 (MQ=255)

                           cgTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCt                                                                                                                     >  1:1637748/1‑131 (MQ=255)

                            gTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGc                                                                                                           >  1:236853/1‑140 (MQ=255)

                            gTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATT‑CCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCa                                                                                                          <  1:469476/140‑1 (MQ=255)

                                   gaAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCg                                                                                                    <  2:490667/140‑1 (MQ=255)

                                   gaAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCg                                                                                                    <  1:232045/140‑1 (MQ=255)

                                   aaaaGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCg                                                                                                    <  2:319428/139‑1 (MQ=255)

                                      aGCGCAGGCGTTTGTACGAGGTTCACGATGAGTGGGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCgct                                                                                                  >  1:948171/1‑140 (MQ=255)

                                      aGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCgctt                                                                                                 >  2:26618/1‑139 (MQ=255)

                                      aGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGCTGCGCACCATCTAATGGCTCAGGGGTGGCAAGTTCgccg                                                                                                 >  2:2345539/1‑138 (MQ=255)

                                         gcAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCgctggct                                                                                              <  1:1468113/140‑1 (MQ=255)

                                             gCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTgggg                                                                                          >  2:1771795/1‑140 (MQ=255)

                                              ggTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGa                                                                                         <  2:993534/139‑1 (MQ=255)

                                                tttGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACt                                                                                       >  1:2393401/1‑140 (MQ=255)

                                                  tGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATTGCTCAGGGCTGGCAAGTTCGCTGGCTGGGGGCTGc                                                                                     >  2:2225697/1‑140 (MQ=255)

                                                  tGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGc                                                                                     <  1:411789/140‑1 (MQ=255)

                                                   gTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGcc                                                                                    <  2:1582556/140‑1 (MQ=255)

                                                   gTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGcc                                                                                    <  2:236853/140‑1 (MQ=255)

                                                     aCGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGa                                                                                  <  2:2393401/140‑1 (MQ=255)

                                                     aCGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGa                                                                                  <  1:951207/140‑1 (MQ=255)

                                                     aCGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGa                                                                                  <  2:2035028/140‑1 (MQ=255)

                                                      cGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGAc                                                                                 <  1:2061958/140‑1 (MQ=255)

                                                          gTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTa                                                                             <  2:1041704/140‑1 (MQ=255)

                                                          gTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCGGTTGTGCACCATCTAATTGCTCAGGGGTGGGAAGGTTGCTGGCTGGGGGCTGTCGACCGTa                                                                             >  1:2359802/1‑140 (MQ=255)

                                                          gTTCACGATGAGT‑GGTCAAGGAAAGCGATTAAAGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGtt                                                                             >  2:69432/1‑139 (MQ=255)

                                                           ttCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCAGGGACTGGCTGTTGCGCACCACCTAATGGGTCCGGGTTGGCAAGTTCGCTGGCTGCGGACTGCCGACCGTAt                                                                            >  2:2241049/1‑140 (MQ=255)

                                                            tCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATg                                                                           <  1:189535/140‑1 (MQ=255)

                                                                    aGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCggg                                                                   >  2:2139494/1‑139 (MQ=255)

                                                                    aGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGa                                                                   >  2:163346/1‑140 (MQ=255)

                                                                     gT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGGTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGAc                                                                  >  2:1349225/1‑140 (MQ=255)

                                                                        ggTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACtt                                                                <  2:1979200/140‑1 (MQ=255)

                                                                         gTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTa                                                               <  1:1694322/140‑1 (MQ=255)

                                                                                 aaGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCaaa                                                       >  1:364743/1‑140 (MQ=255)

                                                                                  aGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTTTTCCCGGGACTGGCTGATGCGCACCATCTAATGGCCTAGGGTTGACAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGAATTAGTGCCaaaa                                                      >  2:2489563/1‑140 (MQ=255)

                                                                                  aGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCaaaa                                                      <  2:1918680/140‑1 (MQ=255)

                                                                                   gCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGGCTGCCGACCGTTTGGAAGCGGACTTAGTGCCaaaaa                                                     >  2:42336/1‑139 (MQ=255)

                                                                                   gCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACtt                                                                <  2:1325274/129‑1 (MQ=255)

                                                                                   gCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACtt                                                                >  1:1325274/1‑129 (MQ=255)

                                                                                    cGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACa                                                    >  1:1605098/1‑140 (MQ=255)

                                                                                              tGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGaa                                          >  1:659356/1‑140 (MQ=255)

                                                                                                aTGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAt                                        >  1:656549/1‑140 (MQ=255)

                                                                                                aTGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAt                                        >  2:959389/1‑140 (MQ=255)

                                                                                                aTGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAt                                        <  2:2321149/140‑1 (MQ=255)

                                                                                                 tGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAtt                                       >  1:950584/1‑140 (MQ=255)

                                                                                                 tGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAtt                                       >  2:2467016/1‑140 (MQ=255)

                                                                                                 tGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAAtt                                       <  1:163346/140‑1 (MQ=255)

                                                                                                  ggCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTg                                      >  2:2180256/1‑140 (MQ=255)

                                                                                                     aGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGAtt                                   <  1:1720155/140‑1 (MQ=255)

                                                                                                     aGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGAtt                                   <  2:364743/140‑1 (MQ=255)

                                                                                                      ggCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGAttt                                  <  2:1605098/140‑1 (MQ=255)

                                                                                                      ggCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGAttt                                  <  2:1380968/140‑1 (MQ=255)

                                                                                                       gCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCTGACTTAGTGCCAAAACATGGCATCGAAATTGATTTc                                 >  1:2342843/1‑140 (MQ=255)

                                                                                                       gCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTc                                 >  1:493458/1‑140 (MQ=255)

                                                                                                       gCGGAACCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCCCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGAtttt                                 >  2:1365499/1‑139 (MQ=255)

                                                                                                          gAACCGGTGGACATGTATTCCCGGGACTGACTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCAtt                              <  1:1653066/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGGCTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  1:2139494/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  2:656549/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  2:493458/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  2:1007038/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  2:431877/140‑1 (MQ=255)

                                                                                                            aCCGGTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCg                            <  2:659356/140‑1 (MQ=255)

                                                                                                                gTGGACATGTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGGAATTGATTTCATTCGTAtt                        >  2:2188997/1‑139 (MQ=255)

                                                                                                                        gTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCt                >  2:595951/1‑140 (MQ=255)

                                                                                                                        gTATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCt                >  2:2494515/1‑140 (MQ=255)

                                                                                                                         tATTCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTg               <  1:2467016/140‑1 (MQ=255)

                                                                                                                           ttCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCg             >  2:1712379/1‑140 (MQ=255)

                                                                                                                           ttCCCGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCg             <  1:2480050/140‑1 (MQ=255)

                                                                                                                              ccGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTgg          <  1:592156/140‑1 (MQ=255)

                                                                                                                               cGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGa         <  1:1771795/140‑1 (MQ=255)

                                                                                                                               cGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGa         <  1:1579325/140‑1 (MQ=255)

                                                                                                                               cGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGa         <  1:26618/140‑1 (MQ=255)

                                                                                                                               cGGGACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGa         <  1:2345539/140‑1 (MQ=255)

                                                                                                                                 ggACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGaaa       >  2:218685/1‑140 (MQ=255)

                                                                                                                                 ggACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGaaa       >  1:75451/1‑140 (MQ=255)

                                                                                                                                 ggACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGaaa       <  1:69432/140‑1 (MQ=255)

                                                                                                                                  gACTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGaaaa      <  2:1419737/140‑1 (MQ=255)

                                                                                                                                   aCTGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAg     >  1:1267062/1‑140 (MQ=255)

                                                                                                                                     tGGCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGt   >  2:762676/1‑140 (MQ=255)

                                                                                                                                      ggCTGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGta  <  1:62063/140‑1 (MQ=255)

ATGCTGTTGCGTATTGATTATGAAACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGAGT‑GGTCAAGGAAAGCGATTAATGGTGATGGCAGGCGGAACCGGTGGACATGTATTCCCGGGACTGGCGGTTGCGCACCATCTAATGGCTCAGGGTTGGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTA  >  NC_000913/99579‑99851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A