Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F48 I1 R1	62	147.6	5031800	99.1%	4986513	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	371,618	G→T	W127L (TGG→TTG)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	371,618	0	G	T	100.0%	421.4 / NA	118	W127L (TGG→TTG)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (65/53); total (65/53)

Only 100 of 119 total aligned reads displayed.

TTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCT  >  NC_000913/371486‑371753

ttAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAg                                                                                                                                  <  2:2133908/140‑1 (MQ=255)

ttAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAg                                                                                                                                  >  2:2468315/1‑140 (MQ=255)

      gTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTACTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTc                                                                                                                            >  1:2132632/1‑140 (MQ=255)

        ggTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCgg                                                                                                                          <  1:662692/140‑1 (MQ=255)

         gTTCGCGATCGGATCTTAATGTACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGc                                                                                                                         >  1:909485/1‑140 (MQ=255)

         gTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGc                                                                                                                         >  1:486134/1‑140 (MQ=255)

         gTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGc                                                                                                                         >  1:419807/1‑140 (MQ=255)

           tCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCgg                                                                                                                          <  2:794980/137‑1 (MQ=255)

           tCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCgg                                                                                                                          >  1:794980/1‑137 (MQ=255)

           tCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaa                                                                                                                       >  2:1948893/1‑140 (MQ=255)

           tCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaa                                                                                                                       >  1:190885/1‑140 (MQ=255)

            cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaaa                                                                                                                      >  1:241300/1‑140 (MQ=255)

            cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaaa                                                                                                                      >  2:748484/1‑140 (MQ=255)

            cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaaa                                                                                                                      >  1:58131/1‑140 (MQ=255)

            cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCaaa                                                                                                                      <  2:1985508/140‑1 (MQ=255)

             gcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAAc                                                                                                                     <  2:486134/140‑1 (MQ=255)

             gcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAAc                                                                                                                     <  2:545615/140‑1 (MQ=255)

              cgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGGCGGCAAACt                                                                                                                    >  2:291223/1‑140 (MQ=255)

               gATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTg                                                                                                                   >  1:10216/1‑140 (MQ=255)

                  cGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTg                                                                                                                <  1:638882/140‑1 (MQ=255)

                   ggATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGc                                                                                                               >  2:1686187/1‑140 (MQ=255)

                         tAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATgg                                                                                                         >  1:1671650/1‑140 (MQ=255)

                          aaTGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATggg                                                                                                        <  2:619953/140‑1 (MQ=255)

                           aTGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggc                                                                                                       >  2:478528/1‑140 (MQ=255)

                           aTGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggc                                                                                                       >  2:1283286/1‑140 (MQ=255)

                           aTGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggc                                                                                                       >  2:1128323/1‑140 (MQ=255)

                             gCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGATCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcgg                                                                                                     <  2:1257063/140‑1 (MQ=255)

                              aaCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcggc                                                                                                    <  2:39574/139‑1 (MQ=255)

                               aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTTCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGgcggcg                                                                                                   >  1:2467452/1‑140 (MQ=255)

                               aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGgcggcg                                                                                                   >  1:769237/1‑140 (MQ=255)

                               aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGgcggcg                                                                                                   >  1:2381509/1‑140 (MQ=255)

                               aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGgcggcg                                                                                                   >  2:1270021/1‑140 (MQ=255)

                                  aaTCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCTACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGCCGGCGGTa                                                                                                >  2:1985451/1‑140 (MQ=255)

                                  aaTCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa                                                                                                <  2:419807/140‑1 (MQ=255)

                                  aaTCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa                                                                                                >  1:116988/1‑140 (MQ=255)

                                  aaTCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa                                                                                                <  2:769237/140‑1 (MQ=255)

                                  aaTCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa                                                                                                >  2:61342/1‑140 (MQ=255)

                                          aaaGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGggcggc                                                                                        <  1:728277/140‑1 (MQ=255)

                                           aaGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCa                                                                                       <  1:1804620/140‑1 (MQ=255)

                                           aaGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCa                                                                                       <  1:2468315/140‑1 (MQ=255)

                                           aaGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCa                                                                                       <  2:2471375/140‑1 (MQ=255)

                                            aGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCAt                                                                                      <  2:190885/140‑1 (MQ=255)

                                             gCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATg                                                                                     >  2:2080973/1‑140 (MQ=255)

                                              cGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCa                                                                                       <  2:785121/137‑1 (MQ=255)

                                              cGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCa                                                                                       >  1:785121/1‑137 (MQ=255)

                                               gtggtgGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGggcggc                                                                                        >  2:1764587/1‑135 (MQ=255)

                                               gtggtgGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGggcggc                                                                                        <  1:1764587/135‑1 (MQ=255)

                                               gtggtgGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAg                                                                                   <  1:1431888/140‑1 (MQ=255)

                                                tggtggATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgt                                                                                  <  1:748484/140‑1 (MQ=255)

                                                tggtggATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgt                                                                                  >  1:1878717/1‑140 (MQ=255)

                                                 ggtggATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtt                                                                                 >  1:1195323/1‑140 (MQ=255)

                                                   tggATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttg                                                                               >  1:1098789/1‑140 (MQ=255)

                                                     gATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt                                                                             <  2:10216/140‑1 (MQ=255)

                                                             ggATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGa                                                                     >  2:2161140/1‑140 (MQ=255)

                                                               atatCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATg                                                                   <  1:291223/140‑1 (MQ=255)

                                                                 atCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGcc                                                                 >  2:2200040/1‑140 (MQ=255)

                                                                   cGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCa                                                                >  1:1376953/1‑139 (MQ=255)

                                                                   cGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCa                                                                <  2:1376953/139‑1 (MQ=255)

                                                                    gCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAAc                                                              >  1:2035262/1‑140 (MQ=255)

                                                                    gCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAAc                                                              <  2:58131/140‑1 (MQ=255)

                                                                     ccAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAAcc                                                             >  1:1146365/1‑140 (MQ=255)

                                                                     ccAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAAcc                                                             >  1:1875132/1‑140 (MQ=255)

                                                                     ccAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAAcc                                                             >  2:2197356/1‑140 (MQ=255)

                                                                      cAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCg                                                            <  1:415481/140‑1 (MQ=255)

                                                                      cAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCg                                                            <  1:1948893/140‑1 (MQ=255)

                                                                      aaaaaTCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCg                                                            <  2:731302/139‑1 (MQ=255)

                                                                       aaaaTCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGa                                                           <  2:241300/140‑1 (MQ=255)

                                                                        aaaTCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGaa                                                          <  2:2381509/140‑1 (MQ=255)

                                                                                   ctgGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCg                                               <  1:1669019/140‑1 (MQ=255)

                                                                                          aCTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAAt                                        <  1:2080973/140‑1 (MQ=255)

                                                                                          aCTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAAt                                        <  1:2161140/140‑1 (MQ=255)

                                                                                            tCGATGGGTGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCa                                      <  2:2467452/140‑1 (MQ=255)

                                                                                            tCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCa                                      <  2:1671650/140‑1 (MQ=255)

                                                                                                tGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCtt                                  >  1:2295547/1‑140 (MQ=255)

                                                                                                   ggggCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTAt                               <  2:116988/140‑1 (MQ=255)

                                                                                                     ggCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCg                             >  2:1150010/1‑140 (MQ=255)

                                                                                                      gCCATAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGt                            >  1:1237261/1‑140 (MQ=255)

                                                                                                         aTAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAg                         >  2:2021662/1‑140 (MQ=255)

                                                                                                          tAGTTCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGc                        >  2:1967057/1‑140 (MQ=255)

                                                                                                             ttCTGTGGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGa                     <  1:1985451/140‑1 (MQ=255)

                                                                                                                tgtgGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTa                  >  1:441962/1‑140 (MQ=255)

                                                                                                                 gtgGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTAt                 >  2:467795/1‑140 (MQ=255)

                                                                                                                  tgGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATc                >  1:1027195/1‑140 (MQ=255)

                                                                                                                  tgGCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATc                >  1:1736986/1‑140 (MQ=255)

                                                                                                                    gCGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGa              <  1:478528/140‑1 (MQ=255)

                                                                                                                    gCGTTCACCCTTAAATTGCCGGAGCGCGTCGGAAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGa              >  1:1474506/1‑140 (MQ=255)

                                                                                                                     cGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaa             >  1:1237862/1‑140 (MQ=255)

                                                                                                                     cGTTCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaa             >  2:611912/1‑140 (MQ=255)

                                                                                                                       ttCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaaaa           >  1:1546716/1‑140 (MQ=255)

                                                                                                                       ttCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaaaa           >  2:1386929/1‑140 (MQ=255)

                                                                                                                       ttCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaaaa           >  1:2090162/1‑140 (MQ=255)

                                                                                                                       ttCACCCTTAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGaaaa           >  1:413049/1‑140 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAGCTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGAATGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  1:2197356/140‑1 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  2:1257149/140‑1 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  1:1128323/140‑1 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  1:1150010/140‑1 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  2:1237261/140‑1 (MQ=255)

                                                                                                                              ttAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAg    <  1:2021662/140‑1 (MQ=255)

                                                                                                                               tAAATTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGc   <  1:611912/140‑1 (MQ=255)

                                                                                                                                aaaTTGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAACTTTATCGTCAGCCGACTATCGAAAACCTGAAGCt  <  2:1878717/140‑1 (MQ=255)

TTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCT  >  NC_000913/371486‑371753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A