Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F48 I1 R1	62	147.6	5031800	99.1%	4986513	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	603,786	G→T	P293H (CCT→CAT)	ybdG ←	miniconductance mechanosensitive channel YbdG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	603,786	0	G	T	100.0%	579.2 / NA	160	P293H (CCT→CAT)	ybdG	miniconductance mechanosensitive channel YbdG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (80/80); total (80/80)

Only 100 of 163 total aligned reads displayed.

ATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTCCAGTTTTTAAAGG  >  NC_000913/603662‑603921

atatTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcg                                                                                                                          >  1:2121588/1‑140 (MQ=255)

atatTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcg                                                                                                                          <  1:2499723/140‑1 (MQ=255)

atatTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcg                                                                                                                          >  2:370648/1‑140 (MQ=255)

 tatTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcgc                                                                                                                         <  2:1287349/140‑1 (MQ=255)

  atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCt                                                                                                                        <  1:1079122/140‑1 (MQ=255)

  atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCt                                                                                                                        >  1:235877/1‑140 (MQ=255)

  atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCt                                                                                                                        >  1:281618/1‑140 (MQ=255)

  atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCt                                                                                                                        >  2:1894079/1‑140 (MQ=255)

        tCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTc                                                                                                                  <  1:381062/140‑1 (MQ=255)

         cAGATAGGCACGAAAGGTTCCAATATTGGTCATTAGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTct                                                                                                                 >  2:294479/1‑139 (MQ=255)

          aGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAg                                                                                                                >  2:855947/1‑140 (MQ=255)

          aGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAg                                                                                                                >  1:2456707/1‑140 (MQ=255)

            aTAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGAc                                                                                                              <  1:1713015/140‑1 (MQ=255)

             tAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACg                                                                                                             <  2:1724679/140‑1 (MQ=255)

              aGGTACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGt                                                                                                            <  1:2216995/140‑1 (MQ=255)

                gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcgc                                                                                                                         >  2:1733389/1‑125 (MQ=255)

                gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATgcgc                                                                                                                         <  1:1733389/125‑1 (MQ=255)

                gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGttt                                                                                                          >  2:1326391/1‑139 (MQ=255)

                 cACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGc                                                                                                         >  1:1843070/1‑140 (MQ=255)

                 cACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGc                                                                                                         >  1:2299068/1‑140 (MQ=255)

                    gAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAACGCGCTTTATTCAGACGTTGCAtt                                                                                                      <  1:599012/140‑1 (MQ=255)

                         gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGtt                                                                                                 >  2:680325/1‑139 (MQ=255)

                         gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTc                                                                                                 <  1:2505691/140‑1 (MQ=255)

                         gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTc                                                                                                 <  1:1647676/140‑1 (MQ=255)

                          ttCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCt                                                                                                >  2:235840/1‑140 (MQ=255)

                          ttCCAATATTGGTCATTCGGCGCACATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCt                                                                                                >  2:1127639/1‑140 (MQ=255)

                           tCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCtt                                                                                               >  1:636457/1‑140 (MQ=255)

                           tCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCtt                                                                                               >  1:2194267/1‑140 (MQ=255)

                                 atTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCaa                                                                                         <  1:1736379/140‑1 (MQ=255)

                                  ttGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAg                                                                                        <  2:1143073/140‑1 (MQ=255)

                                    ggacATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaa                                                                                      >  1:1900357/4‑140 (MQ=255)

                                    ggTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaa                                                                                      >  2:291720/1‑140 (MQ=255)

                                    ggTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaa                                                                                      >  1:257957/1‑140 (MQ=255)

                                     gTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaaa                                                                                     >  2:1625113/1‑140 (MQ=255)

                                     gTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaaa                                                                                     >  1:2408841/1‑140 (MQ=255)

                                       cATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAAc                                                                                   <  2:2428608/140‑1 (MQ=255)

                                       cATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAAc                                                                                   <  1:1312941/140‑1 (MQ=255)

                                          tCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGaa                                                                                >  2:1513824/1‑140 (MQ=255)

                                            ggCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATa                                                                              <  2:2299068/140‑1 (MQ=255)

                                            ggCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATa                                                                              <  1:2294536/140‑1 (MQ=255)

                                              cgcAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCACTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACt                                                                            >  1:799743/1‑140 (MQ=255)

                                                cAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTgg                                                                          >  1:2377149/1‑140 (MQ=255)

                                                 aGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGt                                                                         <  1:1880659/140‑1 (MQ=255)

                                                 aGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGt                                                                         <  1:1418721/140‑1 (MQ=255)

                                                   aTTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGa                                                                       <  2:418878/140‑1 (MQ=255)

                                                   aTTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGa                                                                       <  2:636457/140‑1 (MQ=255)

                                                    tttAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGAc                                                                      <  2:634615/140‑1 (MQ=255)

                                                      tAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACAt                                                                    <  1:11418/140‑1 (MQ=255)

                                                      tAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCCCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACAt                                                                    <  1:1709464/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  2:1843070/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:222722/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:1031887/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:1338282/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  2:165630/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:1130133/140‑1 (MQ=255)

                                                       aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:1518866/140‑1 (MQ=255)

                                                         tACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCaa                                                                 <  2:2456707/140‑1 (MQ=255)

                                                         tACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCaa                                                                 <  1:180080/140‑1 (MQ=255)

                                                            cGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatac                                                              <  1:1315190/140‑1 (MQ=255)

                                                            cGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAataa                                                              <  2:2377149/140‑2 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATTCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  2:864947/1‑140 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGt                                                                         <  2:2014992/128‑1 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGt                                                                         >  1:2014992/1‑128 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:2026741/134‑1 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   >  2:1262760/1‑134 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   >  2:2026741/1‑134 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  1:1262760/134‑1 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  2:264849/1‑140 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  2:91970/1‑140 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  1:332503/1‑140 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  1:2487793/1‑140 (MQ=255)

                                                             gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  2:1585721/1‑140 (MQ=255)

                                                             caCTCCGTAGAACCTTTGGTGCGATTCCACTCATTAATTTCCTGATGACGGCCGGTTAAATAAGGCTTTAACAAATTCGCGTTATTTAGATGTTTCATTGCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                                             >  2:2366628/2‑140 (MQ=255)

                                                                tCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgat                                                          >  1:1953334/1‑140 (MQ=255)

                                                                tCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgat                                                          <  2:1451392/140‑1 (MQ=255)

                                                                 ccGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgata                                                         <  1:1894079/140‑1 (MQ=255)

                                                                  cGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                                                   <  2:1109775/129‑1 (MQ=255)

                                                                   gTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatact                                                       <  1:2224371/140‑1 (MQ=255)

                                                                   gTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatact                                                       <  2:2460994/140‑1 (MQ=255)

                                                                   gTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatact                                                       <  2:2353483/140‑1 (MQ=255)

                                                                     aGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTgc                                                     <  1:544080/140‑1 (MQ=255)

                                                                        aCCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCt                                                  <  1:370648/140‑1 (MQ=255)

                                                                         ccTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCtt                                                 >  1:1679499/1‑140 (MQ=255)

                                                                            tGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAAt                                              >  1:690997/1‑140 (MQ=255)

                                                                             gCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATa                                             <  1:879176/140‑1 (MQ=255)

                                                                                   gATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCACTTAATACGTcgc                                       >  1:2256310/1‑140 (MQ=255)

                                                                                       ccACTCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgcccgc                                   <  1:1585721/140‑1 (MQ=255)

                                                                                           tCATTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGa                               <  1:415013/140‑1 (MQ=255)

                                                                                             aTTAATTTCCTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATg                             >  2:1354820/1‑140 (MQ=255)

                                                                                                      cTGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATcc                    <  2:2252446/140‑1 (MQ=255)

                                                                                                       tGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATccc                   >  2:528823/1‑140 (MQ=255)

                                                                                                       tGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATccc                   >  2:1032376/1‑140 (MQ=255)

                                                                                                       tGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATccc                   >  2:2114842/1‑140 (MQ=255)

                                                                                                       tGATGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATccc                   >  1:1507257/1‑140 (MQ=255)

                                                                                                         aTGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGc                 >  1:675764/1‑140 (MQ=255)

                                                                                                         aTGGCGGCTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGc                 >  2:1486648/1‑140 (MQ=255)

                                                                                                            gcggcTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTcc              >  2:178733/1‑140 (MQ=255)

                                                                                                             cggcTGGTTAAATAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATCCTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTCCa             <  2:1347698/140‑1 (MQ=255)

                                                                                                                       aataatGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCACTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTCCAGTTTTTAAAg   <  2:2256310/140‑1 (MQ=255)

                                                                                                                        aTAATGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAACCGAATCCTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTCCAGTTTTTAAAgg  <  1:2366628/140‑1 (MQ=255)

ATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACATCCCGCTCCAGTTTTTAAAGG  >  NC_000913/603662‑603921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A