Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F48 I1 R1 62 147.6 5031800 99.1% 4986513 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 636,335 G→T L79M (CTG→ATG)  ybdN ← putative PAPS reductase/DUF3440 domain‑containing protein YbdN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913636,3350GT98.6% 507.3 / ‑0.4 142L79M (CTG→ATG) ybdNputative PAPS reductase/DUF3440 domain‑containing protein YbdN
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (95/45);  total (96/46)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 142 total aligned reads displayed.

GGAGGTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAAT  >  NC_000913/636199‑636471
                                                                                                                                        |                                                                                                                                        
ggAGGTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGa                                                                                                                                       <  1:452539/140‑1 (MQ=255)
ggAGGTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGa                                                                                                                                       <  1:349135/140‑1 (MQ=255)
 gAGGTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGAc                                                                                                                                      <  2:1184706/140‑1 (MQ=255)
   ggTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTg                                                                                                                                    >  1:1690539/1‑140 (MQ=255)
    gTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGa                                                                                                                                   >  2:2327809/1‑140 (MQ=255)
     ttGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGaa                                                                                                                                  >  2:2270122/1‑140 (MQ=255)
     ttGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCAGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGaa                                                                                                                                  <  1:1009498/140‑1 (MQ=255)
      tGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAAc                                                                                                                                 <  1:2228632/140‑1 (MQ=255)
      tGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAAc                                                                                                                                 <  1:2227512/140‑1 (MQ=255)
      tGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAAc                                                                                                                                 <  1:1872406/140‑1 (MQ=255)
      tGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAAc                                                                                                                                 <  2:39914/140‑1 (MQ=255)
        aCGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACAt                                                                                                                               >  2:1209855/1‑140 (MQ=255)
             cccATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTa                                                                                                                          >  2:260468/1‑140 (MQ=255)
             cccATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTa                                                                                                                          >  1:2159384/1‑140 (MQ=255)
              ccATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTaa                                                                                                                         <  1:1961712/140‑1 (MQ=255)
              ccATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCACAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTaa                                                                                                                         <  2:762539/140‑1 (MQ=255)
                 ttCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATgg                                                                                                                      >  1:160928/1‑140 (MQ=255)
                  tCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGt                                                                                                                     >  1:541809/1‑140 (MQ=255)
                   cGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTg                                                                                                                    <  1:794357/140‑1 (MQ=255)
                        tCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAaga                                                                                                               >  2:466929/1‑140 (MQ=255)
                          aGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAgaga                                                                                                             <  1:260468/140‑1 (MQ=255)
                               cccAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTgc                                                                                                        <  1:2270122/140‑1 (MQ=255)
                                ccAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTgcg                                                                                                       <  1:1437741/140‑1 (MQ=255)
                                ccAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCATCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTgcg                                                                                                       >  2:1427558/1‑140 (MQ=255)
                                       tGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCa                                                                                                >  2:1481320/1‑140 (MQ=255)
                                       tGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCa                                                                                                >  2:510566/1‑140 (MQ=255)
                                       tGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCa                                                                                                >  2:83598/1‑140 (MQ=255)
                                        gCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAg                                                                                               <  2:601347/140‑1 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAg                                                                                               >  2:1849127/1‑139 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  2:1004960/1‑140 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  1:429410/1‑140 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  2:938407/1‑140 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  1:212066/1‑140 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  1:1772683/1‑140 (MQ=255)
                                         ccATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGt                                                                                              >  2:1697216/1‑140 (MQ=255)
                                           aTTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCg                                                                                            <  1:96990/140‑1 (MQ=255)
                                              cGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATa                                                                                         >  2:1006932/1‑140 (MQ=255)
                                                 gTTGGTATTGTGAAAGGGAATTTTGCGTCGGAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAAc                                                                                      >  1:1801131/1‑140 (MQ=255)
                                                   tGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACaa                                                                                    >  2:2462417/1‑140 (MQ=255)
                                                           tgAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGa                                                                            >  2:1882121/1‑140 (MQ=255)
                                                           tgAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGGGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGa                                                                            >  2:453161/1‑140 (MQ=255)
                                                               aGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAACCGCAGAtttt                                                                        >  2:1533353/1‑140 (MQ=255)
                                                               aGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAtttt                                                                        >  1:1316604/1‑140 (MQ=255)
                                                               aGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAtttt                                                                        >  1:518637/1‑140 (MQ=255)
                                                               aGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAtttt                                                                        >  1:1605812/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  2:1816077/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  2:755498/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  1:1074884/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  1:682400/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  1:131819/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttt                                                                       >  2:1710165/1‑140 (MQ=255)
                                                                gggAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCAAGTCGATAAACAAAACGCAGAttttt                                                                       >  2:1921351/1‑140 (MQ=255)
                                                                  gAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGAttttttt                                                                     >  1:253206/1‑140 (MQ=255)
                                                                     ttttGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGcc                                                                  <  1:2013794/140‑1 (MQ=255)
                                                                        tGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCAt                                                               >  2:2253756/1‑140 (MQ=255)
                                                                            tcgtAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGt                                                           <  2:541809/140‑1 (MQ=255)
                                                                            tcgtAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGt                                                           <  2:727231/140‑1 (MQ=255)
                                                                               tAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCgg                                                        >  1:678931/1‑140 (MQ=255)
                                                                                      gAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGtt                                                 <  2:1735772/140‑1 (MQ=255)
                                                                                       aGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTc                                                >  1:1026085/1‑140 (MQ=255)
                                                                                       aGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTc                                                >  1:2404118/1‑140 (MQ=255)
                                                                                         cgcAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCgg                                              >  2:2454352/1‑140 (MQ=255)
                                                                                         cgcAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCgg                                              >  2:2028061/1‑140 (MQ=255)
                                                                                         cgcAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCgg                                              <  2:131819/140‑1 (MQ=255)
                                                                                         cgcAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCgg                                              <  1:2253756/140‑1 (MQ=255)
                                                                                         cgcAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCgg                                              >  1:232364/1‑140 (MQ=255)
                                                                                           cAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCt                                            >  1:2469200/1‑140 (MQ=255)
                                                                                                cAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAg                                       >  2:505395/1‑140 (MQ=255)
                                                                                                   tAAAACTCTTCGATGACATCGGTGTCCAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATg                                    <  1:755498/140‑1 (MQ=255)
                                                                                                   tAAAACTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATg                                    <  1:175776/140‑1 (MQ=255)
                                                                                                   tAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATg                                    <  1:1697216/140‑1 (MQ=255)
                                                                                                    aaaaCTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgc                                   <  2:212066/140‑1 (MQ=255)
                                                                                                    aaaaCTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgc                                   <  1:1006932/140‑1 (MQ=255)
                                                                                                    aaaaCTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgc                                   <  1:2089385/140‑1 (MQ=255)
                                                                                                    aaaaCTCTTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgc                                   <  2:1885579/140‑1 (MQ=255)
                                                                                                        ctctTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCGAGTTCGGCTGTCAGATgcagca                               >  1:2103284/1‑140 (MQ=255)
                                                                                                        ctctTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgcagca                               >  1:1646985/1‑140 (MQ=255)
                                                                                                        ctctTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATgcagca                               >  1:1383824/1‑140 (MQ=255)
                                                                                                          ctTCGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCAtt                             <  2:232364/140‑1 (MQ=255)
                                                                                                             cGATGACATCGGTGTACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTaaa                          >  2:1961713/1‑140 (MQ=255)
                                                                                                                        gtgtACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCttt               >  1:1632019/1‑140 (MQ=255)
                                                                                                                           tACAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTgcc            >  1:1229525/1‑140 (MQ=255)
                                                                                                                             cAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTgccgc          >  1:1364975/1‑140 (MQ=255)
                                                                                                                             cAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTgccgc          >  2:340906/1‑140 (MQ=255)
                                                                                                                             cAACTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTgccgc          >  2:246561/1‑140 (MQ=255)
                                                                                                                                cTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCat       >  2:212274/1‑139 (MQ=255)
                                                                                                                                cTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAg       >  2:1740661/1‑140 (MQ=255)
                                                                                                                                cTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAg       >  2:159951/1‑140 (MQ=255)
                                                                                                                                cTCGCGCATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAg       >  1:1782684/1‑140 (MQ=255)
                                                                                                                                  cgcgCATGGACTGAACATAGTTAATGTTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCTGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaa     >  2:1769019/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  2:2019821/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  1:1655515/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  1:1818624/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  1:521820/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  2:2162571/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  1:350809/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  2:855879/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  2:933697/1‑140 (MQ=255)
                                                                                                                                   gcgcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGaaa    >  1:2371051/1‑140 (MQ=255)
                                                                                                                                     gcATGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAAt  >  2:1882604/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GGAGGTTGACGTACCCATTCGACATCAGGTTCCCAGCACTGCCATTCGGGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAAT  >  NC_000913/636199‑636471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A