Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F48 I1 R1 62 147.6 5031800 99.1% 4986513 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 751,168 G→T P334P (CCC→CCA)  ybgQ ← putative fimbrial usher protein YbgQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,1680GT99.2% 445.3 / ‑4.7 125P334P (CCC→CCA) ybgQputative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (60/64);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 127 total aligned reads displayed.

AGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGG  >  NC_000913/751034‑751300
                                                                                                                                      |                                                                                                                                    
aGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGt                                                                                                                                 <  1:1821322/140‑1 (MQ=255)
    aGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGAt                                                                                                                             >  2:2110461/1‑140 (MQ=255)
    aGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGAt                                                                                                                             >  1:58916/1‑140 (MQ=255)
    aGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGAt                                                                                                                             >  1:2357444/1‑140 (MQ=255)
         tttGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGAtgctgc                                                                                                                        >  1:131845/1‑140 (MQ=255)
            gagaGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGa                                                                                                                     <  1:1316503/140‑1 (MQ=255)
            gagaGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGa                                                                                                                     <  1:2146845/140‑1 (MQ=255)
              aagCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGaaa                                                                                                                   <  2:748750/139‑1 (MQ=255)
                 cATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCt                                                                                                                >  1:2478485/1‑140 (MQ=255)
                 cATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCt                                                                                                                >  2:1401219/1‑140 (MQ=255)
                 cATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCt                                                                                                                >  2:1045536/1‑140 (MQ=255)
                 cATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCt                                                                                                                >  1:477529/1‑140 (MQ=255)
                 cATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCt                                                                                                                >  1:397807/1‑140 (MQ=255)
                 cATCCCACAGTCAACTTCATTGCTAAAAAAGGGTTCATTTTCAGGTTGCTGTCACATGGAAGGCTGCCGCTTACCCGCAGCCAATTTATAGCGAACCGGTCCCTGGCGAGTCAGGACTTGCGTCTATGCTGCCGAAACCt                                                                                                                >  2:2175992/1‑140 (MQ=255)
                   tCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTgg                                                                                                              >  1:148058/1‑140 (MQ=255)
                   tCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTgg                                                                                                              >  1:281403/1‑140 (MQ=255)
                         aGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTg                                                                                                        <  2:349394/140‑1 (MQ=255)
                           gAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGtt                                                                                                      <  2:736113/140‑1 (MQ=255)
                           gAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGtt                                                                                                      >  1:1594491/1‑140 (MQ=255)
                               cTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGGTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAcc                                                                                                  <  1:1045474/140‑1 (MQ=255)
                               cTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAcc                                                                                                  >  2:2327849/1‑140 (MQ=255)
                               cTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAcc                                                                                                  >  2:476018/1‑140 (MQ=255)
                               cTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAcc                                                                                                  <  1:1519435/140‑1 (MQ=255)
                                ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAccc                                                                                                 >  1:2170797/1‑140 (MQ=255)
                                ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAccc                                                                                                 >  2:622260/1‑140 (MQ=255)
                                ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAccc                                                                                                 >  1:179892/1‑140 (MQ=255)
                                ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAccc                                                                                                 >  1:403157/1‑140 (MQ=255)
                                  cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGTTGCCGAAACCTGGAAATTGTTCACCCGa                                                                                               <  2:1695093/140‑1 (MQ=255)
                                  cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGa                                                                                               <  1:2110461/140‑1 (MQ=255)
                                  cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGa                                                                                               <  2:1129686/140‑1 (MQ=255)
                                  cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGa                                                                                               <  2:325910/140‑1 (MQ=255)
                                  cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGa                                                                                               <  2:469133/140‑1 (MQ=255)
                                   aTTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGAc                                                                                              <  1:541352/140‑1 (MQ=255)
                                    ttGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGAcc                                                                                             <  1:2088154/140‑1 (MQ=255)
                                     tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCa                                                                                            >  2:578006/1‑140 (MQ=255)
                                      gCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCAt                                                                                           >  1:1582213/1‑140 (MQ=255)
                                               ggTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCg                                                                                  >  2:489643/1‑140 (MQ=255)
                                                gTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                                                                 <  1:1045536/140‑1 (MQ=255)
                                                gTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                                                                 <  1:622260/140‑1 (MQ=255)
                                                 tttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTc                                                                                >  1:215358/1‑140 (MQ=255)
                                                 tttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTc                                                                                >  1:1038183/1‑140 (MQ=255)
                                                 tttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTc                                                                                >  1:2317659/1‑140 (MQ=255)
                                                  ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCa                                                                               <  2:512076/140‑1 (MQ=255)
                                                  ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCa                                                                               <  2:1473973/140‑1 (MQ=255)
                                                  ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCa                                                                               <  1:500386/140‑1 (MQ=255)
                                                   tCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCAc                                                                              >  2:2449547/1‑140 (MQ=255)
                                                   tCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCAc                                                                              >  2:17388/1‑140 (MQ=255)
                                                           aGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACAt                                                                      <  1:89460/140‑1 (MQ=255)
                                                             tttGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATcc                                                                    >  1:493358/1‑140 (MQ=255)
                                                              ttGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCa                                                                   <  2:2347417/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  2:403157/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  2:2317659/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  2:1652821/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  1:186445/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  2:2170797/140‑1 (MQ=255)
                                                                gATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                                                 <  1:2449547/140‑1 (MQ=255)
                                                                    tgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGc                                                             <  2:1978533/140‑1 (MQ=255)
                                                                    tgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGc                                                             <  2:360648/140‑1 (MQ=255)
                                                                     gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGcc                                                            <  2:138833/140‑1 (MQ=255)
                                                                     gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGcc                                                            <  1:2175992/140‑1 (MQ=255)
                                                                     gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGcc                                                            <  1:2327849/140‑1 (MQ=255)
                                                                          ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCCCCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                       <  1:1987726/140‑1 (MQ=255)
                                                                          ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                       <  2:1273997/140‑1 (MQ=255)
                                                                          ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                       <  2:215358/140‑1 (MQ=255)
                                                                          ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                       <  2:2149763/140‑1 (MQ=255)
                                                                          ggAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa                                                       <  1:173062/140‑1 (MQ=255)
                                                                              ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                                                   <  2:179892/140‑1 (MQ=255)
                                                                                ccGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                                                 >  1:1210822/1‑140 (MQ=255)
                                                                                 cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACtt                                                >  2:167179/1‑139 (MQ=255)
                                                                                  gTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGa                                               >  1:1817557/1‑140 (MQ=255)
                                                                                   tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGAt                                              >  2:1724027/1‑140 (MQ=255)
                                                                                      cTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGa                                           >  1:1294928/1‑140 (MQ=255)
                                                                                        gACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAgg                                         <  2:2328296/140‑1 (MQ=255)
                                                                                         aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGt                                        >  2:2045366/1‑140 (MQ=255)
                                                                                         aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGt                                        >  1:692936/1‑140 (MQ=255)
                                                                                         aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGt                                        >  2:494473/1‑140 (MQ=255)
                                                                                          cccGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtc                                       >  2:759413/1‑140 (MQ=255)
                                                                                           ccGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtca                                      <  2:774465/140‑1 (MQ=255)
                                                                                            ggcgGTCAATTTATAGCGAACCTGTCCCTGTCGAGACAGGAATGGAGTCGATGCTGCCAAAACCTGGACATTGTTCACCCGACCTTGTTCTACCGTCACTTTGACATACAGCGTGACCTGAACAGATTGAATGAGGtctt                                     <  2:2373393/139‑3 (MQ=255)
                                                                                            cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                     <  2:692936/140‑1 (MQ=255)
                                                                                            cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                     <  2:477529/140‑1 (MQ=255)
                                                                                            cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                     <  2:1817557/140‑1 (MQ=255)
                                                                                            cgcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcat                                     <  2:397807/140‑1 (MQ=255)
                                                                                             gcgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatc                                    <  1:1401219/140‑1 (MQ=255)
                                                                                              cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatca                                   >  2:1986131/1‑140 (MQ=255)
                                                                                              cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatca                                   >  2:277790/1‑140 (MQ=255)
                                                                                              cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatca                                   >  1:783122/1‑140 (MQ=255)
                                                                                                gCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCaat                                 <  1:476018/140‑1 (MQ=255)
                                                                                                    aTTTATAGCGAACCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAataata                             >  2:1543907/1‑140 (MQ=255)
                                                                                                               aCCTGTCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTgt                  >  2:380067/1‑139 (MQ=255)
                                                                                                                    tCCCTGGCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGa             <  1:1724027/140‑1 (MQ=255)
                                                                                                                         ggCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttt        >  1:2387966/1‑140 (MQ=255)
                                                                                                                         ggCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttt        >  2:282645/1‑140 (MQ=255)
                                                                                                                         ggCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttt        <  2:1240514/140‑1 (MQ=255)
                                                                                                                          gCGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACttttt       <  1:7519/140‑1 (MQ=255)
                                                                                                                           cGAGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACtttttt      <  1:1724889/140‑1 (MQ=255)
                                                                                                                             aGTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCt    >  2:761919/1‑140 (MQ=255)
                                                                                                                              gTCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGGTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCATGGCTTTTTTCtt   >  2:1280627/1‑139 (MQ=255)
                                                                                                                               tCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTgg  >  1:2460814/1‑140 (MQ=255)
                                                                                                                               tCAGGAATGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTgg  >  2:2466146/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
AGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGG  >  NC_000913/751034‑751300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A