Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	810,479	G→T	W33C (TGG→TGT)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	810,479	0	G	T	100.0%	156.0 / NA	45	W33C (TGG→TGT)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (21/24); total (21/24)

CCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCT  >  NC_000913/810344‑810606

ccccGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCTGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGctgtctgg                                                                                                                             >  2:744677/1‑140 (MQ=255)

   cGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCtggtgg                                                                                                                          >  1:914519/1‑140 (MQ=255)

    ggACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGc                                                                                                                         <  2:779821/140‑1 (MQ=255)

    ggACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGCCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGc                                                                                                                         <  2:694771/140‑1 (MQ=255)

          cgacgaATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGTTGGCGgatga                                                                                                                   >  2:42838/1‑140 (MQ=255)

           gacgaATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGgatgat                                                                                                                  >  1:778305/1‑140 (MQ=255)

             cgaATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTACTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATct                                                                                                                >  2:305782/1‑139 (MQ=255)

                atatTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCa                                                                                                             <  2:824097/140‑1 (MQ=255)

                    tgccaCGCGACAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGGGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTAt                                                                                                         <  2:872075/136‑1 (MQ=255)

                      cAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCt                                                                                                       >  1:330796/1‑140 (MQ=255)

                      cAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCt                                                                                                       >  1:351826/1‑140 (MQ=255)

                         cgcgGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGaa                                                                                                    >  2:850044/1‑140 (MQ=255)

                          gcgGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAAc                                                                                                   >  1:1043783/1‑140 (MQ=255)

                          gcgGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAAc                                                                                                   >  1:851270/1‑140 (MQ=255)

                           cgGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACt                                                                                                  >  2:251319/1‑140 (MQ=255)

                            ggCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACtt                                                                                                 >  1:758674/1‑140 (MQ=255)

                             gcagcaTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACttt                                                                                                >  1:700496/1‑140 (MQ=255)

                              cagcaTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACtttt                                                                                               >  1:658747/1‑140 (MQ=255)

                              cagcaTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACtttt                                                                                               <  1:524579/140‑1 (MQ=255)

                                 caTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCa                                                                                            <  2:1043783/140‑1 (MQ=255)

                                   ttATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGt                                                                                          <  2:778305/140‑1 (MQ=255)

                                      tGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAAc                                                                                       <  2:560274/140‑1 (MQ=255)

                                      tGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGCTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAAc                                                                                       >  2:713084/1‑140 (MQ=255)

                                         gCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGat                                                                                    <  2:710860/140‑1 (MQ=255)

                                           tGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGattt                                                                                  >  1:812246/1‑139 (MQ=255)

                                           tGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGatta                                                                                  >  1:273441/1‑140 (MQ=255)

                                            ggCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGattat                                                                                 <  1:833839/140‑1 (MQ=255)

                                             gCAGGAGAAAATCAACGCTGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGattatt                                                                                <  1:744677/140‑1 (MQ=255)

                                             gCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGattatt                                                                                <  1:153582/140‑1 (MQ=255)

                                             gCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGattatt                                                                                >  1:756155/1‑140 (MQ=255)

                                              cAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTAttt                                                                               <  2:658747/140‑1 (MQ=255)

                                                  agaAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGt                                                                           <  2:758674/140‑1 (MQ=255)

                                                       aTCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAg                                                                      >  1:522044/1‑140 (MQ=255)

                                                           aCGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCcat                                                                  <  1:850044/140‑1 (MQ=255)

                                                               ggcgCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCatcatc                                                              <  1:824462/140‑1 (MQ=255)

                                                                 cgcTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCg                                                            >  1:765938/1‑140 (MQ=255)

                                                                              gcgTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTg                                               <  1:42838/140‑1 (MQ=255)

                                                                                         aTGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGcagcag                                    >  2:687878/1‑140 (MQ=255)

                                                                                             ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAggggg                                <  1:251319/140‑1 (MQ=255)

                                                                                                 cGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGa                            >  1:991257/1‑140 (MQ=255)

                                                                                                  gTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAg                           >  2:756347/1‑140 (MQ=255)

                                                                                                  gTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCATGGCAGCAGGGGGCGGAg                           <  1:687878/140‑1 (MQ=255)

                                                                                                      ccGTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAAt                       <  2:756155/140‑1 (MQ=255)

                                                                                                        gTTATCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAAttt                     <  2:330796/140‑1 (MQ=255)

                                                                                                           aTCCGGTGGCGCAAGGAGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGc                  <  2:700496/140‑1 (MQ=255)

                                                                                                                           aGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGTCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCt  <  2:507464/140‑1 (MQ=255)

                                                                                                                           aGCCGGACGCTGTCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCt  <  2:251543/140‑1 (MQ=255)

CCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCT  >  NC_000913/810344‑810606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A