Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A20 F48 I1 R1 52 61.9 2103308 98.8% 2078068 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 845,414 Δ1 bp coding (67/2226 nt) ybiO ← moderate conductance mechanosensitive channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,4130C.100.0% 240.4 / NA 56coding (68/2226 nt)ybiOmoderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/24);  total (32/24)

AACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAAT  >  NC_000913/845275‑845547
                                                                                                                                          |                                                                                                                                      
aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcg                                                                                                                                       <  1:609772/140‑2 (MQ=255)
aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcg                                                                                                                                       <  1:725329/140‑2 (MQ=255)
aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcg                                                                                                                                       <  2:605785/140‑2 (MQ=255)
  cTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcggg                                                                                                                                     <  1:403601/140‑4 (MQ=255)
     gTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTAt                                                                                                                                 >  1:673309/1‑140 (MQ=255)
     gTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTAt                                                                                                                                 >  1:938520/1‑140 (MQ=255)
     gTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTAt                                                                                                                                 >  2:402290/1‑140 (MQ=255)
         aTCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGAt                                                                                                                             <  1:112564/140‑1 (MQ=255)
          tCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcg                                                                                                                                       >  2:125609/1‑129 (MQ=255)
          tCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGcg                                                                                                                                       <  1:125609/130‑2 (MQ=255)
              ctctTTCCGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACcga                                                                                                                        <  2:756420/140‑2 (MQ=255)
                    aCGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGc                                                                                                                  >  2:674878/1‑140 (MQ=255)
                    aCGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGc                                                                                                                  >  2:858678/1‑140 (MQ=255)
                        gAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAggg                                                                                                              >  1:472744/1‑139 (MQ=255)
                        gAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGt                                                                                                              >  2:393511/1‑140 (MQ=255)
                          ggTGTCATTATCCAACACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTgc                                                                                                            <  1:952887/140‑1 (MQ=255)
                           gtgtCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTTGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTgcg                                                                                                           >  1:298270/1‑140 (MQ=255)
                           gtgtCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTgcg                                                                                                           >  1:786639/1‑140 (MQ=255)
                               cATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCca                                                                                                       <  1:255079/140‑1 (MQ=255)
                                    tCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGCTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGTATACCGCGTGGGCAGGTGCGCCcagcagc                                                                                                  >  2:289697/1‑139 (MQ=255)
                                      cAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                                                                                                >  2:173675/1‑140 (MQ=255)
                                      cAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                                                                                                >  2:964982/1‑140 (MQ=255)
                                      cAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                                                                                                >  1:517503/1‑140 (MQ=255)
                                        ccagaTCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATGTCCGGTTCCCGGGCCGGTCCAGTCGTTGAGTCAGTTGTCGTTGTGGTTGTAACG‑CGGGTGTGAATACCGCGTGGGAACCTGCTCCCAtcagccaca                                                                                              >  2:796155/5‑138 (MQ=255)
                                         cacaTCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                                                                                             >  1:800283/1‑140 (MQ=255)
                                         cacaTCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                                                                                             >  1:933779/1‑140 (MQ=255)
                                          acaTCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaag                                                                                            <  2:524885/140‑1 (MQ=255)
                                          acaTCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaag                                                                                            >  2:189070/1‑140 (MQ=255)
                                             tCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAgg                                                                                         <  1:698875/140‑1 (MQ=255)
                                              ccGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGa                                                                                        >  1:71923/1‑140 (MQ=255)
                                              ccGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGa                                                                                        >  1:1041554/1‑140 (MQ=255)
                                              ccGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGa                                                                                        >  1:406480/1‑140 (MQ=255)
                                              ccGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGa                                                                                        >  1:899595/1‑140 (MQ=255)
                                               cGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTTGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGAt                                                                                       <  2:298270/140‑1 (MQ=255)
                                                    gTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACa                                                                                  <  1:402290/140‑1 (MQ=255)
                                                     tGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCTTTGTCTTTGTGGTTGTAAC‑TCGGGTATGGATACCGCGTGGGCAGGGGGGCCCAGCAGCCAGAATAGGAGTAACAg                                                                                 >  2:648731/1‑140 (MQ=255)
                                                       cgcCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGa                                                                               >  1:502607/1‑140 (MQ=255)
                                                         ccATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATc                                                                             <  2:786639/140‑1 (MQ=255)
                                                         ccATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATc                                                                             <  1:479641/140‑1 (MQ=255)
                                                         ccATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATc                                                                             <  2:71923/140‑1 (MQ=255)
                                                          cATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATcc                                                                            >  1:940759/1‑140 (MQ=255)
                                                              ggCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCg                                                                        <  2:938520/140‑1 (MQ=255)
                                                                 cGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCAt                                                                     <  2:896326/140‑1 (MQ=255)
                                                                    ttttttttGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGTTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGCGCGCCCAGCAGCCAGAAGAGGATGAACAGGATCCACCGCATGAc                                                                  >  2:244580/1‑140 (MQ=255)
                                                                      ttttttGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGActc                                                                >  1:530677/1‑140 (MQ=255)
                                                                               cGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTga                                                       <  1:189070/140‑1 (MQ=255)
                                                                                            cGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCaa                                          >  1:12926/1‑140 (MQ=255)
                                                                                            cGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCaa                                          >  2:130895/1‑140 (MQ=255)
                                                                                            cGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCaa                                          >  1:937710/1‑140 (MQ=255)
                                                                                             ggggCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCaaa                                         <  2:517503/140‑1 (MQ=255)
                                                                                                gCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAg                                      >  1:897703/1‑140 (MQ=255)
                                                                                                  cGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGAt                                    <  2:502607/140‑1 (MQ=255)
                                                                                                  cGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGAt                                    <  1:442740/140‑1 (MQ=255)
                                                                                                  cGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGAt                                    <  2:899595/140‑1 (MQ=255)
                                                                                                  cGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGAt                                    <  1:130895/140‑1 (MQ=255)
                                                                                                   ggTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATa                                   >  1:382331/1‑140 (MQ=255)
                                                                                                               gAGTCCGTTGTCGTTGTGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCt                       >  2:866264/1‑140 (MQ=255)
                                                                                                                      ttgtcgttgtGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGgaggag                >  1:922799/1‑140 (MQ=255)
                                                                                                                       tgtcgttgtGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGaggagg               >  1:472678/1‑140 (MQ=255)
                                                                                                                        gtcgttgtGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAggg              <  2:1041554/140‑1 (MQ=255)
                                                                                                                        gtcgttgtGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAggg              >  1:604506/1‑140 (MQ=255)
                                                                                                                          cgttgtGGTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGc            <  2:922799/140‑1 (MQ=255)
                                                                                                                                 gTTGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTa     <  2:604506/140‑1 (MQ=255)
                                                                                                                                   tGTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGACGATTTTaaa   <  1:77119/140‑1 (MQ=255)
                                                                                                                                    gTAACG‑CGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAAt  <  2:940759/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
AACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAAT  >  NC_000913/845275‑845547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A