Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,367	C→A	M166I (ATG→ATT)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,367	0	C	A	99.0%	353.9 / ‑4.6	105	M166I (ATG→ATT)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (48/55); total (49/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 107 total aligned reads displayed.

AAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCC  >  NC_000913/80233‑80503

aaaTCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTTCGGTAATGCCCGGCGCTGCTTTGCCCTGGACTTCAATTTTAATGGt                                                                                                                                     >  1:141019/1‑140 (MQ=255)

 aaTCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGtt                                                                                                                                    >  2:1481265/1‑140 (MQ=255)

  aTCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGttt                                                                                                                                   >  1:775515/1‑140 (MQ=255)

  aTCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGttt                                                                                                                                   >  2:538767/1‑140 (MQ=255)

  aTCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGttt                                                                                                                                   >  2:2046504/1‑140 (MQ=255)

     aCGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTg                                                                                                                                <  1:1531740/140‑1 (MQ=255)

      cGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgc                                                                                                                               >  1:350754/1‑140 (MQ=255)

      cGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgc                                                                                                                               >  2:1486725/1‑140 (MQ=255)

      cGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgc                                                                                                                               >  2:188464/1‑140 (MQ=255)

       ggATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGGCCGGCGCGGCTTTGTCCTGGACTTCAATTTTAATGGTTTTTgcg                                                                                                                              <  1:1569940/140‑1 (MQ=255)

       ggATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgcg                                                                                                                              <  2:284450/140‑1 (MQ=255)

       ggATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgcg                                                                                                                              <  1:1657733/140‑1 (MQ=255)

       ggATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgcg                                                                                                                              >  2:148786/1‑140 (MQ=255)

        gATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgcgc                                                                                                                             <  1:724542/140‑1 (MQ=255)

        gATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTgcgc                                                                                                                             <  1:538767/140‑1 (MQ=255)

         aTTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGcgcg                                                                                                                            <  1:1436785/140‑1 (MQ=255)

            gCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGcc                                                                                                                         <  2:490583/140‑1 (MQ=255)

            gCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGcc                                                                                                                         <  1:1481265/140‑1 (MQ=255)

            gCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGcc                                                                                                                         <  1:184126/140‑1 (MQ=255)

            gCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGcc                                                                                                                         <  2:2095724/140‑1 (MQ=255)

            gCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGcc                                                                                                                         <  1:985123/140‑1 (MQ=255)

              ttCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCt                                                                                                                       <  1:1226884/140‑1 (MQ=255)

                  ccgcAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGttt                                                                                                                   <  1:717810/140‑1 (MQ=255)

                  ccgcAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGttt                                                                                                                   >  2:1060475/1‑140 (MQ=255)

                         aCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGtt                                                                                                            <  1:118122/140‑1 (MQ=255)

                         aCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGtt                                                                                                            <  1:617468/140‑1 (MQ=255)

                            ccaccaCATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGc                                                                                                         <  1:444281/140‑1 (MQ=255)

                             caccacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGct                                                                                                        >  2:163438/1‑139 (MQ=255)

                             caccacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCg                                                                                                        <  2:683674/140‑1 (MQ=255)

                             caccacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCACGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCg                                                                                                        >  1:235195/1‑140 (MQ=255)

                               ccacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGtt                                                                                                      >  1:1110343/1‑140 (MQ=255)

                                          gTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtg                                                                                           >  2:389918/1‑140 (MQ=255)

                                           tGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGt                                                                                                >  1:735992/1‑134 (MQ=255)

                                           tGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGt                                                                                                <  2:735992/134‑1 (MQ=255)

                                            gccgccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGtt                                                                                         <  1:1761608/140‑1 (MQ=255)

                                            gccgccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGtt                                                                                         <  2:2088789/140‑1 (MQ=255)

                                            gccgccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCAGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGtt                                                                                         <  1:1397596/140‑1 (MQ=255)

                                             ccgccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTc                                                                                        <  1:1959711/140‑1 (MQ=255)

                                               gccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTTATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCaa                                                                                      >  1:1977336/1‑140 (MQ=255)

                                                     gcTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCgg                                                                                <  1:163438/140‑1 (MQ=255)

                                                       tACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGaa                                                                              <  1:1060475/140‑1 (MQ=255)

                                                       tACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGaa                                                                              <  1:1362323/140‑1 (MQ=255)

                                                       tACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGaa                                                                              <  1:1834196/140‑1 (MQ=255)

                                                              tttACCGATAATTGCCAGCACGATATCTTTTGTGGTAATGCCCGGCGCGGCTTTGCACTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGAGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       <  2:141019/140‑1 (MQ=255)

                                                              tttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       <  1:1522236/140‑1 (MQ=255)

                                                              tttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       <  2:575459/140‑1 (MQ=255)

                                                              tttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       <  2:775515/140‑1 (MQ=255)

                                                                 aCCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       <  2:686240/137‑1 (MQ=255)

                                                                 aCCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                                       >  1:686240/1‑137 (MQ=255)

                                                                 aCCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATAc                                                                    >  2:842763/1‑140 (MQ=255)

                                                                       aaTTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAgg                                                              >  2:435262/1‑140 (MQ=255)

                                                                         ttGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGcc                                                            >  2:1782964/1‑140 (MQ=255)

                                                                               gCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTgcg                                                      >  1:2062768/1‑140 (MQ=255)

                                                                                cACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTgcgc                                                     >  2:621899/1‑140 (MQ=255)

                                                                                cACGATATCTTTTGCGGTAATGCCCGGCACGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTgcgc                                                     <  2:235195/140‑1 (MQ=255)

                                                                                  cGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCa                                                   <  1:1486725/140‑1 (MQ=255)

                                                                                    atatCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCaaa                                                 >  1:1656863/1‑140 (MQ=255)

                                                                                    atatCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCaaa                                                 >  2:503649/1‑140 (MQ=255)

                                                                                     tatCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAAc                                                <  1:2141827/140‑1 (MQ=255)

                                                                                          tttGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGcc                                           >  1:1409146/1‑140 (MQ=255)

                                                                                            tGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgt                                         >  1:94758/1‑140 (MQ=255)

                                                                                            tGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgt                                         >  1:1111469/1‑140 (MQ=255)

                                                                                             gCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                                        >  1:1658444/1‑140 (MQ=255)

                                                                                             gCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                                        >  1:176320/1‑140 (MQ=255)

                                                                                               ggTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtggg                                      <  1:621899/140‑1 (MQ=255)

                                                                                                gTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggt                                     <  2:1656863/140‑1 (MQ=255)

                                                                                                gTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggt                                     <  2:1354205/140‑1 (MQ=255)

                                                                                                gTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGCGCGCCAAACGCGCCgtgggt                                     <  1:1673476/140‑1 (MQ=255)

                                                                                                 tAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggtg                                    >  1:1529384/1‑140 (MQ=255)

                                                                                                  aaTGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggtgg                                   <  1:1028290/140‑1 (MQ=255)

                                                                                                          gcgcGGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGc                           >  2:1354213/1‑140 (MQ=255)

                                                                                                           cgcgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATgtg                          >  1:1476767/1‑138 (MQ=255)

                                                                                                           cgcgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCg                          >  1:244082/1‑140 (MQ=255)

                                                                                                           cgcgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCg                          >  1:2009233/1‑140 (MQ=255)

                                                                                                           cgcgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCg                          >  2:1376591/1‑140 (MQ=255)

                                                                                                           cgcgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCg                          >  2:296936/1‑140 (MQ=255)

                                                                                                             cgGCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAg                        <  2:1274348/140‑1 (MQ=255)

                                                                                                              ggCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGt                       <  2:2130184/140‑1 (MQ=255)

                                                                                                               gCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTc                      >  1:1616247/1‑140 (MQ=255)

                                                                                                               gCTTTGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTc                      >  2:847183/1‑140 (MQ=255)

                                                                                                                  ttGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTcgcc                   <  1:1376591/140‑1 (MQ=255)

                                                                                                                   tGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTcgccg                  <  2:1586805/140‑1 (MQ=255)

                                                                                                                   tGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTcgccg                  <  2:1678851/140‑1 (MQ=255)

                                                                                                                   tGCCCTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTcgccg                  <  1:959566/140‑1 (MQ=255)

                                                                                                                     cccTGGACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCa                <  2:1110343/140‑1 (MQ=255)

                                                                                                                          gACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACaa           >  1:1304897/1‑140 (MQ=255)

                                                                                                                          gACTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCATGGGTGGCGGTATGCGAGTCGCCGCAGACaa           >  1:1211547/1‑140 (MQ=255)

                                                                                                                            cTTCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATg         <  2:2119722/140‑1 (MQ=255)

                                                                                                                              tCAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGt       <  2:350754/140‑1 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGt                       >  1:1405916/1‑123 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGt                       <  2:1405916/123‑1 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  2:1200927/1‑140 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  1:648223/1‑140 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  1:2073737/1‑140 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  2:2006037/1‑140 (MQ=255)

                                                                                                                               cAATTTTAATGGTTTTTGCGCGGCCCTGTTTCAGGGGTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  1:2080212/1‑140 (MQ=255)

                                                                                                                               cAATTTTAATGGGTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTc      >  2:1895440/1‑140 (MQ=255)

                                                                                                                                  ttttAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATc   <  2:1658444/140‑1 (MQ=255)

                                                                                                                                  ttttAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATc   <  2:94758/140‑1 (MQ=255)

                                                                                                                                   tttAATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATcc  <  1:148786/140‑1 (MQ=255)

AAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCC  >  NC_000913/80233‑80503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A