Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,831	G→T	S41S (TCC→TCA)	yadC ←	fimbrial tip‑adhesin YadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,831	0	G	T	100.0%	807.1 / NA	231	S41S (TCC→TCA)	yadC	fimbrial tip‑adhesin YadC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (95/136); total (95/136)

Only 100 of 233 total aligned reads displayed.

TAAAGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATG  >  NC_000913/150694‑150966

tAAAGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGa                                                                                                                                       >  2:1178557/1‑140 (MQ=255)

tAAAGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGa                                                                                                                                       >  2:1117471/1‑140 (MQ=255)

  aaGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAgg                                                                                                                                     <  2:951893/140‑1 (MQ=255)

  aaGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAgg                                                                                                                                     <  1:1846144/140‑1 (MQ=255)

  aaGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAgg                                                                                                                                     <  2:2140296/140‑1 (MQ=255)

     aaTACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGc                                                                                                                                  <  1:1763129/140‑1 (MQ=255)

       tACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCt                                                                                                                                >  2:1246810/1‑140 (MQ=255)

       tACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCt                                                                                                                                <  2:1076948/140‑1 (MQ=255)

       tACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCt                                                                                                                                >  1:189781/1‑140 (MQ=255)

       tACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCt                                                                                                                                >  2:1836797/1‑140 (MQ=255)

        accaccaccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTc                                                                                                                               >  1:757570/1‑140 (MQ=255)

          caccaccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGa                                                                                                                             >  1:161070/1‑140 (MQ=255)

          caccaccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGa                                                                                                                             >  1:2051384/1‑140 (MQ=255)

          caccaccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGa                                                                                                                             >  2:832465/1‑140 (MQ=255)

               ccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGtt                                                                                                                        >  2:2056502/1‑140 (MQ=255)

               ccGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGtt                                                                                                                        >  1:1688897/1‑140 (MQ=255)

                  gTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATc                                                                                                                     >  2:1101306/1‑140 (MQ=255)

                       tGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTc                                                                                                                <  2:1316905/140‑1 (MQ=255)

                       tGTATCCAGGTACGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTc                                                                                                                <  2:1257071/140‑1 (MQ=255)

                             cAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTg                                                                                                             >  1:1082572/1‑137 (MQ=255)

                               ggTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCatta                                                                                                        <  2:161070/140‑1 (MQ=255)

                               ggTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCatta                                                                                                        >  1:1204362/1‑140 (MQ=255)

                                gTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCattat                                                                                                       >  1:1261329/1‑140 (MQ=255)

                                   tGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTc                                                                                                    >  1:1706608/1‑140 (MQ=255)

                                   tGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTc                                                                                                    >  2:2078922/1‑140 (MQ=255)

                                   tGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTc                                                                                                    >  1:971644/1‑140 (MQ=255)

                                     acaaGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  <  1:1091915/137‑1 (MQ=255)

                                     aCCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  >  1:1540868/1‑140 (MQ=255)

                                     aCCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  >  1:666129/1‑140 (MQ=255)

                                     aCCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  >  2:469523/1‑140 (MQ=255)

                                     aCCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  >  2:925229/1‑140 (MQ=255)

                                     aCCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                                                                                  >  1:274969/1‑140 (MQ=255)

                                       cAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCcac                                                                                                <  1:1246810/140‑1 (MQ=255)

                                          tACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAAt                                                                                             >  2:1194739/1‑140 (MQ=255)

                                          tACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAAt                                                                                             >  1:1428110/1‑140 (MQ=255)

                                          tACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAAt                                                                                             >  1:113749/1‑140 (MQ=255)

                                            aaTTTTTGCCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATaa                                                                                           <  2:1802517/139‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  2:189781/140‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  1:1104427/140‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  2:328094/140‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  2:1688897/140‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  1:261051/140‑1 (MQ=255)

                                                tttACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                                                                                       <  1:214620/140‑1 (MQ=255)

                                                   aCCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTNt                                                                                    >  2:1773466/1‑140 (MQ=255)

                                                    ccccAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGta                                                                                   <  2:235330/140‑1 (MQ=255)

                                                     cccAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGtat                                                                                  <  1:186196/140‑1 (MQ=255)

                                                     cccAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGtat                                                                                  <  2:58124/140‑1 (MQ=255)

                                                       cAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGtat                                                                                  >  2:1523059/1‑138 (MQ=255)

                                                        aGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATg                                                                               <  1:832465/140‑1 (MQ=255)

                                                         gTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGta                                                                                   >  2:1434103/1‑135 (MQ=255)

                                                         gTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGc                                                                              >  1:1129789/1‑140 (MQ=255)

                                                         atCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGc                                                                              <  1:1274833/139‑1 (MQ=255)

                                                          tCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCa                                                                             <  1:597706/140‑1 (MQ=255)

                                                          tCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCa                                                                             <  1:2078922/140‑1 (MQ=255)

                                                            gggCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCact                                                                           <  2:708138/140‑1 (MQ=255)

                                                            gggCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCact                                                                           <  2:1755738/140‑1 (MQ=255)

                                                              gCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  2:1558781/140‑1 (MQ=255)

                                                              gCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  2:1706608/140‑1 (MQ=255)

                                                              gCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  2:113749/140‑1 (MQ=255)

                                                              gCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  1:569729/140‑1 (MQ=255)

                                                              gCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  2:442753/140‑1 (MQ=255)

                                                                  aGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         >  1:489118/1‑136 (MQ=255)

                                                                  aGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCactga                                                                         <  2:489118/136‑1 (MQ=255)

                                                                  aGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTACCCACAATAAAACTTGTATATGCACTGACTGt                                                                     <  1:1537071/140‑1 (MQ=255)

                                                                     tGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTAtt                                                                  <  2:1328337/140‑1 (MQ=255)

                                                                       ggTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  1:618425/139‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:1931883/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:384773/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:572976/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:757570/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:797011/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:971644/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  1:399327/140‑1 (MQ=255)

                                                                       cGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTAGTTCCCACAATAAAACTTGTATATGCACTGACTGTATTac                                                                <  2:715355/140‑1 (MQ=255)

                                                                         tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTacaa                                                              <  1:1121569/140‑1 (MQ=255)

                                                                         tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTacaa                                                              <  1:1555414/140‑1 (MQ=255)

                                                                         tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTacaa                                                              <  2:607590/140‑1 (MQ=255)

                                                                         tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTacaa                                                              <  1:1948898/140‑1 (MQ=255)

                                                                            gTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTAt                                                                   >  1:581089/1‑132 (MQ=255)

                                                                             tttATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAg                                                          >  1:1371566/1‑140 (MQ=255)

                                                                                     aaaCCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATCCAGt                                                  <  2:666129/140‑1 (MQ=255)

                                                                                      aaCCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAg                                                                                                                                      <  2:1552232/55‑1 (MQ=255)

                                                                                             ttgcttgcTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTaaaaa                                          <  2:382347/140‑1 (MQ=255)

                                                                                             ttgcttgcTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTaaaaa                                          <  1:1436914/140‑1 (MQ=255)

                                                                                                  tgctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAGCTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGaa                                     <  1:161741/140‑1 (MQ=255)

                                                                                                  tgctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGaa                                     <  1:1177734/140‑1 (MQ=255)

                                                                                                   gctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAAt                                    <  1:745657/140‑1 (MQ=255)

                                                                                                    ctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATg                                   <  1:709377/140‑1 (MQ=255)

                                                                                                    ctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATg                                   <  1:1048979/140‑1 (MQ=255)

                                                                                                         gATGTGTAATTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCt                              <  1:60930/140‑1 (MQ=255)

                                                                                                                aaTTAAAGGTCATCTGTGCGGCAGTTGAGGGGCCTCGTTAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATa                       <  1:787932/140‑1 (MQ=255)

                                                                                                                    aaaGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCt                   <  1:651652/140‑1 (MQ=255)

                                                                                                                     aaGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCtt                  <  1:46303/140‑1 (MQ=255)

                                                                                                                      aGGTCATCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTc                 >  1:942174/1‑140 (MQ=255)

                                                                                                                           aTCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCAtttt            >  1:34632/1‑140 (MQ=255)

                                                                                                                           aTCTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCAtttt            >  1:163541/1‑140 (MQ=255)

                                                                                                                             cTGTGCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTa          <  1:1179390/140‑1 (MQ=255)

                                                                                                                               gtgCGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACt        <  2:1371566/140‑1 (MQ=255)

                                                                                                                                  cGGCAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCa     <  2:1261199/140‑1 (MQ=255)

                                                                                                                                     cAGTTGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATg  >  2:1447908/1‑140 (MQ=255)

TAAAGAATACCACCACCGGTACCTGTATCCAGGTATGACCAGTACATTTTTACCCCAGTCGGGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATG  >  NC_000913/150694‑150966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A