Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	163,722	G→T	G540C (GGC→TGC)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	163,722	0	G	T	100.0%	377.6 / NA	110	G540C (GGC→TGC)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (51/59); total (51/59)

Only 100 of 112 total aligned reads displayed.

GGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCT  >  NC_000913/163586‑163857

ggCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCa                                                                                                                                      >  1:277862/1‑140 (MQ=255)

ggCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCa                                                                                                                                      >  1:1259512/1‑140 (MQ=255)

ggCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCa                                                                                                                                      >  2:1069684/1‑140 (MQ=255)

    tttGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAgcgc                                                                                                                                  <  1:1808064/140‑1 (MQ=255)

    tttGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAgcgc                                                                                                                                  >  2:358293/1‑140 (MQ=255)

          gATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGcc                                                                                                                            >  2:550642/1‑140 (MQ=255)

           aTCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCg                                                                                                                           >  2:602804/1‑140 (MQ=255)

           aTCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCg                                                                                                                           >  1:1554162/1‑140 (MQ=255)

                aTTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAGCTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATgc                                                                                                                      <  2:1374018/140‑1 (MQ=255)

                aTTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATgc                                                                                                                      <  2:431304/140‑1 (MQ=255)

                 ttGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATgcg                                                                                                                     <  1:1867392/140‑1 (MQ=255)

                 ttGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATgcg                                                                                                                     <  2:1554162/140‑1 (MQ=255)

                   gCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGcgcg                                                                                                                   <  1:602804/140‑1 (MQ=255)

                   gCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGcgcg                                                                                                                   <  2:1905762/140‑1 (MQ=255)

                       gtcgCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGAtt                                                                                                               >  1:701364/1‑140 (MQ=255)

                            cGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACt                                                                                                          >  1:1080482/1‑140 (MQ=255)

                                 gCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGgcgc                                                                                                     <  2:1259512/140‑1 (MQ=255)

                                   aaGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCgctg                                                                                                   <  2:585296/140‑1 (MQ=255)

                                   aaGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCgctg                                                                                                   <  1:787006/140‑1 (MQ=255)

                                   aaGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCgctg                                                                                                   <  2:974185/140‑1 (MQ=255)

                                        ggACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGt                                                                                              <  2:1074920/140‑1 (MQ=255)

                                        ggACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGt                                                                                              <  1:766091/140‑1 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            >  1:1164912/1‑140 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            >  1:2127977/1‑140 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            <  2:167514/140‑1 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            >  2:1797216/1‑140 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            >  1:607925/1‑140 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            <  1:550642/140‑1 (MQ=255)

                                          aCGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCACCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCg                                                                                            >  1:808801/1‑140 (MQ=255)

                                               aTCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATAtt                                                                                       >  1:722575/1‑140 (MQ=255)

                                               aTCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATAtt                                                                                       >  1:612275/1‑140 (MQ=255)

                                               aTCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATAtt                                                                                       >  1:1286324/1‑140 (MQ=255)

                                                 cAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATtga                                                                                     <  1:651819/140‑1 (MQ=255)

                                                 cAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATtga                                                                                     <  2:1355035/140‑1 (MQ=255)

                                                 aaaCTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATtga                                                                                     <  2:1080482/139‑1 (MQ=255)

                                                 aaaCTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATtga                                                                                     <  2:701364/139‑1 (MQ=255)

                                                      ggCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGt                                                                                >  2:1430049/1‑140 (MQ=255)

                                                       gCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGtt                                                                               >  2:279077/1‑140 (MQ=255)

                                                        cAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTa                                                                              >  2:1413928/1‑140 (MQ=255)

                                                          aaCGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGt                                                                            >  1:1860003/1‑140 (MQ=255)

                                                               cATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAAc                                                                       >  2:1379217/1‑140 (MQ=255)

                                                                aTGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACg                                                                      <  1:492839/140‑1 (MQ=255)

                                                                         aggCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  2:1561435/3‑140 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  2:1004284/1‑140 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  1:821147/1‑140 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             <  2:449919/140‑1 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  1:1945411/1‑140 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  1:1728794/1‑140 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             <  1:318578/140‑1 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             <  1:1185874/140‑1 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             <  1:1379217/140‑1 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             <  1:1385202/140‑1 (MQ=255)

                                                                         aTGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGGTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCa                                                             >  1:1409829/1‑140 (MQ=255)

                                                                          tGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAg                                                            <  1:1413928/140‑1 (MQ=255)

                                                                           gCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGGTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGGTGCGCGGATTTTCCTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGc                                                           <  2:957591/140‑1 (MQ=255)

                                                                              cGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTgg                                                        >  1:733924/1‑140 (MQ=255)

                                                                              cGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTgg                                                        <  2:1161234/140‑1 (MQ=255)

                                                                                 tGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAc                                                     >  1:341863/1‑140 (MQ=255)

                                                                                 tGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAc                                                     <  1:1430049/140‑1 (MQ=255)

                                                                                 agCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGACGAGTTAGTACAACGCTGCCCGCAGCTGGTAc                                                     <  2:1286324/139‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCATGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  1:1800114/140‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  2:819176/140‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  2:821147/140‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  1:483111/140‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  2:1860003/140‑1 (MQ=255)

                                                                                   ccAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  2:1164912/140‑1 (MQ=255)

                                                                                    cAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcagc                                                  >  1:1748610/1‑140 (MQ=255)

                                                                                     aaCGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcagca                                                 <  2:341863/140‑1 (MQ=255)

                                                                                        gacgCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTc                                              <  1:1069684/140‑1 (MQ=255)

                                                                                        gacgCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTc                                              <  1:842815/140‑1 (MQ=255)

                                                                                        gacgCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTc                                              <  2:277862/140‑1 (MQ=255)

                                                                                          cgcgCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTg                                            <  1:1119110/140‑1 (MQ=255)

                                                                                          cgcgCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTg                                            <  1:279077/140‑1 (MQ=255)

                                                                                           gcgcTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   <  1:1887787/132‑1 (MQ=255)

                                                                                           gcgcTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTAcag                                                   >  2:1887787/1‑132 (MQ=255)

                                                                                             gcTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGaca                                         <  2:722575/140‑1 (MQ=255)

                                                                                                  gccgccACGAATGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACtgtg                                    <  2:661585/140‑1 (MQ=255)

                                                                                                           aaTGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGAt                           >  2:376565/1‑140 (MQ=255)

                                                                                                           aaTGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGAt                           <  2:607925/140‑1 (MQ=255)

                                                                                                           aaTGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGAt                           <  1:1846899/140‑1 (MQ=255)

                                                                                                            aTGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATg                          <  1:358293/140‑1 (MQ=255)

                                                                                                             tGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGa                         >  1:312396/1‑140 (MQ=255)

                                                                                                             tGGTTGATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTCGAGTGGGATGa                         <  1:1808694/140‑1 (MQ=255)

                                                                                                                  gATCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACgcgc                    <  1:376565/140‑1 (MQ=255)

                                                                                                                   aTCGCACCTTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCa                   <  1:1220951/140‑1 (MQ=255)

                                                                                                                   aTCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCa                   <  2:1728794/140‑1 (MQ=255)

                                                                                                                   aTCGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCa                   >  2:1442362/1‑140 (MQ=255)

                                                                                                                     cGCACCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAg                 >  2:83775/1‑140 (MQ=255)

                                                                                                                        aCCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTa              >  2:1624431/1‑140 (MQ=255)

                                                                                                                        aCCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTa              >  2:764009/1‑140 (MQ=255)

                                                                                                                        aCCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTa              <  2:612275/140‑1 (MQ=255)

                                                                                                                        aCCGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTCTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTa              >  2:246426/1‑140 (MQ=255)

                                                                                                                          cGTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACg            >  2:452601/1‑140 (MQ=255)

                                                                                                                           gTTATTATTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGc           <  2:19503/140‑1 (MQ=255)

                                                                                                                             tattatTGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTg         <  2:2127977/140‑1 (MQ=255)

                                                                                                                              attattGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGa        >  1:393523/1‑140 (MQ=255)

                                                                                                                                  ttGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGc    >  2:1041028/1‑140 (MQ=255)

                                                                                                                                  ttGCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGc    <  1:452601/140‑1 (MQ=255)

                                                                                                                                    gCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCt  >  1:1043986/1‑140 (MQ=255)

                                                                                                                                    gCAGTGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCt  >  2:297659/1‑140 (MQ=255)

GGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCT  >  NC_000913/163586‑163857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A