Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	237,429	C→A	A32D (GCT→GAT)	yafT →	lipoprotein YafT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	237,429	0	C	A	99.6%	775.8 / NA	223	A32D (GCT→GAT)	yafT	lipoprotein YafT
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (107/115); total (107/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

AAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTT  >  NC_000913/237294‑237565

aaaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCaa                                                                                                                                      <  1:813968/140‑1 (MQ=255)

 aaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAg                                                                                                                                     >  2:393293/1‑140 (MQ=255)

 aaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAg                                                                                                                                     <  1:2030450/140‑1 (MQ=255)

 aaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAg                                                                                                                                     <  1:268686/140‑1 (MQ=255)

  atatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGt                                                                                                                                    <  1:885428/140‑1 (MQ=255)

    atTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTca                                                                                                                                  <  1:1355835/140‑1 (MQ=255)

     ttCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcac                                                                                                                                 <  2:1157379/140‑1 (MQ=255)

     ttCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcac                                                                                                                                 <  2:375814/140‑1 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                >  2:69082/1‑140 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                <  2:414185/140‑1 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                <  1:1716357/140‑1 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                >  1:153087/1‑140 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                <  2:1849629/140‑1 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                >  2:1734066/1‑140 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                <  2:1086138/140‑1 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                >  1:1086138/1‑140 (MQ=255)

      tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcaca                                                                                                                                <  2:124305/140‑1 (MQ=255)

       cTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcacac                                                                                                                               <  2:854395/140‑1 (MQ=255)

        tAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcacaca                                                                                                                              >  2:1560675/1‑140 (MQ=255)

        tAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTcacaca                                                                                                                              <  2:2081620/140‑1 (MQ=255)

          aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACaaa                                                                                                                            >  1:1176163/1‑140 (MQ=255)

           tCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAg                                                                                                                           <  2:2012925/140‑1 (MQ=255)

               tATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAat                                                                                                                       >  1:460149/1‑140 (MQ=255)

               tATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAat                                                                                                                       >  2:288020/1‑140 (MQ=255)

                aTGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAata                                                                                                                      >  1:913973/1‑140 (MQ=255)

                aTGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAata                                                                                                                      <  1:599393/140‑1 (MQ=255)

                aTGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAata                                                                                                                      <  2:62612/140‑1 (MQ=255)

                aTGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTC‑CGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAatat                                                                                                                     >  2:781101/1‑140 (MQ=255)

                  gACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATAtt                                                                                                                    <  1:1342291/140‑1 (MQ=255)

                     aGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATtaat                                                                                                                 <  2:1936071/140‑1 (MQ=255)

                       gCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATtaataa                                                                                                               >  2:212571/1‑140 (MQ=255)

                        cGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATaaa                                                                                                              >  1:1993007/1‑140 (MQ=255)

                          aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATaaaaa                                                                                                            <  2:334261/140‑1 (MQ=255)

                            ggAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAaca                                                                                                          <  2:1993007/140‑1 (MQ=255)

                             gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAacac                                                                                                         <  2:1176163/140‑1 (MQ=255)

                              aGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACt                                                                                                        >  2:1417221/1‑140 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     <  1:308749/140‑1 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     >  1:279958/1‑140 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     >  2:699274/1‑140 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     <  1:413850/140‑1 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     >  1:74952/1‑140 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     >  2:1356691/1‑140 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     <  1:1119515/140‑1 (MQ=255)

                                 gtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                                     <  2:565061/140‑1 (MQ=255)

                                  tCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTcccc                                                                                                    >  2:1191043/1‑140 (MQ=255)

                                   ccAATTATGAAGTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                                                                                                   >  2:1689985/1‑140 (MQ=255)

                                       ttATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCaa                                                                                               <  2:500251/140‑1 (MQ=255)

                                       ttATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCaa                                                                                               >  2:1450154/1‑140 (MQ=255)

                                           gAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTga                                                                                           <  1:1191043/140‑1 (MQ=255)

                                           gAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTga                                                                                           <  1:794342/140‑1 (MQ=255)

                                                cAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacagac                                                                                      <  1:212571/140‑1 (MQ=255)

                                                cAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacagac                                                                                      <  2:1800795/140‑1 (MQ=255)

                                                 aaaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacagaca                                                                                     <  2:535793/140‑1 (MQ=255)

                                                 aaaaaaGCTTGGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGTTGAATAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacagaca                                                                                     >  2:1298403/1‑140 (MQ=255)

                                                           tgttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGGCAGACAAAGATTTAtt                                                                           >  1:1765621/1‑140 (MQ=255)

                                                           tgttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTAtt                                                                           >  1:897274/1‑140 (MQ=255)

                                                             ttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCg                                                                         <  2:733964/140‑1 (MQ=255)

                                                                     gtgtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGaa                                                                 >  1:1918435/1‑140 (MQ=255)

                                                                       gtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAc                                                               >  2:919218/1‑140 (MQ=255)

                                                                        tgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGTTGAAAAGAAGAAAAAAGATCAAGTCACACAAAGCAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAcc                                                              >  1:520135/1‑140 (MQ=255)

                                                                        tgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAGAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAcc                                                              >  1:1803902/1‑140 (MQ=255)

                                                                          ttATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCAc                                                            >  1:1029493/1‑140 (MQ=255)

                                                                          ttATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCAc                                                            >  1:1974945/1‑140 (MQ=255)

                                                                            aTTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCActc                                                          >  2:431260/1‑140 (MQ=255)

                                                                               ctcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTgg                                                       >  1:1051171/1‑140 (MQ=255)

                                                                                tcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGc                                                      <  1:960034/140‑1 (MQ=255)

                                                                                  gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGcct                                                    >  2:740250/1‑139 (MQ=255)

                                                                                  gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGc                                                      >  1:379156/1‑138 (MQ=255)

                                                                                  gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCg                                                    <  2:897274/140‑1 (MQ=255)

                                                                                   ctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGt                                                   >  1:1905193/1‑140 (MQ=255)

                                                                                       ttGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCAGGCCGTATcc                                               >  2:18357/1‑140 (MQ=255)

                                                                                          cAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAATAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGaa                                            >  1:1509337/1‑140 (MQ=255)

                                                                                          cAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGaa                                            <  2:1029493/140‑1 (MQ=255)

                                                                                          aaGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAAAAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGaa                                            <  2:198391/139‑1 (MQ=255)

                                                                                             gATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAagag                                         <  1:230205/140‑1 (MQ=255)

                                                                                                gtgCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGat                                      >  2:1666955/1‑140 (MQ=255)

                                                                                                gtgCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAAGat                                      >  1:1331639/1‑140 (MQ=255)

                                                                                                  gCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGatat                                    >  1:736435/1‑140 (MQ=255)

                                                                                                   ccTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATAtt                                   >  2:107475/1‑140 (MQ=255)

                                                                                                   ccTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATAtt                                   >  2:122237/1‑140 (MQ=255)

                                                                                                   ccTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATAtt                                   >  2:1903878/1‑140 (MQ=255)

                                                                                                         aaTCTTCTATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCt                             <  1:1830757/140‑1 (MQ=255)

                                                                                                            cttctATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTgcg                          <  2:1773147/140‑1 (MQ=255)

                                                                                                            cttctATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTgcg                          <  2:344535/140‑1 (MQ=255)

                                                                                                            cttctATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTgcg                          <  1:715661/140‑1 (MQ=255)

                                                                                                              tctATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCt                        <  2:1803902/140‑1 (MQ=255)

                                                                                                              tctATTGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCt                        >  1:1774648/1‑140 (MQ=255)

                                                                                                                  ttGATGAAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGAt                    >  2:229129/1‑140 (MQ=255)

                                                                                                                   tgatgaAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATg                   <  1:1162981/140‑1 (MQ=255)

                                                                                                                   tgatgaAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATg                   <  1:1298403/140‑1 (MQ=255)

                                                                                                                     atgaAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGga                 >  2:278054/1‑140 (MQ=255)

                                                                                                                      agaAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGTTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag                <  1:620724/139‑1 (MQ=255)

                                                                                                                       gaAAAGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgaga               >  2:205402/1‑140 (MQ=255)

                                                                                                                        aaaaGAAGAAAAAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGAt              <  1:122237/140‑1 (MQ=255)

                                                                                                                                  aaaaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAATCCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCcgcg    <  2:1905193/140‑1 (MQ=255)

                                                                                                                                  aaaaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCcgcg    <  1:1560675/140‑1 (MQ=255)

                                                                                                                                  aaaaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCcgcg    <  1:1872880/140‑1 (MQ=255)

                                                                                                                                    aaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGtt  <  1:1607277/140‑1 (MQ=255)

                                                                                                                                    aaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGtt  <  1:653816/140‑1 (MQ=255)

                                                                                                                                    aaGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGtt  <  2:1637602/140‑1 (MQ=255)

AAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTT  >  NC_000913/237294‑237565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A