Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 286,452 G→T S353I (AGC→ATC)  yagG → putative D‑xylonate transporter YagG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913286,4520GT100.0% 333.2 / NA 97S353I (AGC→ATC) yagGputative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/56);  total (41/56)

GCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGT  >  NC_000913/286315‑286587
                                                                                                                                         |                                                                                                                                       
gctgATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCg                                                                                                                                       >  1:1022190/1‑140 (MQ=255)
  tgATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGt                                                                                                                                     >  1:380855/1‑140 (MQ=255)
  tgATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGt                                                                                                                                     >  2:411764/1‑140 (MQ=255)
  tgATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGt                                                                                                                                     >  2:421339/1‑140 (MQ=255)
  tgATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGt                                                                                                                                     >  2:579835/1‑140 (MQ=255)
    aTTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTcg                                                                                                                                   <  1:768155/140‑1 (MQ=255)
       ttCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTcgccg                                                                                                                                <  2:949681/140‑1 (MQ=255)
       ttCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTcgccg                                                                                                                                <  1:96797/140‑1 (MQ=255)
            cACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCg                                                                                                                           <  2:659996/140‑1 (MQ=255)
             aCCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGa                                                                                                                          <  2:705105/140‑1 (MQ=255)
             aCCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGa                                                                                                                          <  2:882306/140‑1 (MQ=255)
             aCCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGa                                                                                                                          <  1:90690/140‑1 (MQ=255)
             aCCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGa                                                                                                                          <  1:511670/140‑1 (MQ=255)
             aCCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGa                                                                                                                          <  1:421339/140‑1 (MQ=255)
               cccGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATGCCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACg                                                                                                                        <  2:187772/140‑1 (MQ=255)
               cccGGACGAGCACATCGCCCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGGCTTCAATTCCACCACGCCGCTGCAGTGGTTGGAGGCTTCTAACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACg                                                                                                                        <  2:1767498/140‑1 (MQ=255)
                 cggcggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACggg                                                                                                                      <  1:1272191/140‑1 (MQ=255)
                 cggcggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACggg                                                                                                                      <  2:1764833/140‑1 (MQ=255)
                  ggcggAGCACATGGCGCTCATTTTTGCCCTCAACATCCTTTCCCTGTTCGCTTTAAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGc                                                                                                                     <  2:130902/140‑1 (MQ=255)
                  ggcggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGc                                                                                                                     <  1:735433/140‑1 (MQ=255)
                    cggAGCACTTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCACCGCCTCGACGGGCTg                                                                                                                   <  1:103546/140‑1 (MQ=255)
                    cggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTg                                                                                                                   <  1:971258/140‑1 (MQ=255)
                    cggAGCACATCGCGCTCATTTTGGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCCCCACGCCGCTGCAGTGGCNGATGGCTNCTGACGTGGTGGACTACGAGGAGNNNCGCATCGGTCGCCGCCTCGACGGGCTg                                                                                                                   <  1:1608013/140‑1 (MQ=255)
                     ggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATCCCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTgg                                                                                                                  <  1:1338080/140‑1 (MQ=255)
                     ggAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTgg                                                                                                                  <  2:255171/140‑1 (MQ=255)
                         cacaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCGCCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              <  1:347017/140‑1 (MQ=255)
                         cacaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              >  1:1296182/1‑140 (MQ=255)
                         cacaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              <  2:1022190/140‑1 (MQ=255)
                         cacaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              <  1:1609333/140‑1 (MQ=255)
                         cacaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              >  2:1662990/1‑140 (MQ=255)
                          acaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTtc                                                                                                             <  1:411764/140‑1 (MQ=255)
                           aaTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTtct                                                                                                            <  2:731912/139‑1 (MQ=255)
                            aTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              <  1:1152276/137‑1 (MQ=255)
                            aTCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGtt                                                                                                              >  2:1152276/1‑137 (MQ=255)
                              cgcgCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCa                                                                                                         <  1:1109358/140‑1 (MQ=255)
                                     aTTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCacctacct                                                                                                  >  2:229627/1‑140 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCCCCTACCTGtt                                                                                               <  2:394168/140‑1 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGtt                                                                                               <  2:1860802/140‑1 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGtt                                                                                               <  2:1493750/140‑1 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGtt                                                                                               <  2:1296182/140‑1 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGtt                                                                                               <  1:1484862/140‑1 (MQ=255)
                                        tttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGtt                                                                                               <  2:380855/140‑1 (MQ=255)
                                         ttGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTc                                                                                              <  2:1380583/140‑1 (MQ=255)
                                          tGCCCCCAACATCCTTTCCCTGCTCGTCTTTAAGGCCACCCCGCCGCTGGAGTGGCTGATGGCTTCGGACCTGCAGGACTACGAAGAGAGCCGCATCGGTCGCTGCCTCGACGGGCTGGTGTTCTCCGCCTACCTGTGCa                                                                                             <  2:1110458/140‑1 (MQ=255)
                                            cccTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGc                                                                                           <  1:1769497/140‑1 (MQ=255)
                                              cctaaCATCCTGTTCCTGTTCGTTTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCt                                                                                         <  2:140181/137‑1 (MQ=255)
                                                 aaCATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGaa                                                                                      >  2:23596/1‑140 (MQ=255)
                                                      cctgttcctgttCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGt                                                                                                <  2:862810/125‑1 (MQ=255)
                                                      cctgttcctgttCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGt                                                                                                >  1:862810/1‑125 (MQ=255)
                                                        tgttcgtgttcgTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGc                                                                               <  2:1314524/140‑1 (MQ=255)
                                                         gttcctgttcGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGcc                                                                              >  1:1313017/1‑140 (MQ=255)
                                                          ttcctgttcGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCt                                                                             >  1:1233686/1‑140 (MQ=255)
                                                           tcctgttcGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTg                                                                            >  2:924145/1‑140 (MQ=255)
                                                                ttcGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGAt                                                                       <  1:1728325/140‑1 (MQ=255)
                                                                ttcGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGAt                                                                       >  2:469894/1‑140 (MQ=255)
                                                                   gTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTgg                                                                    <  2:333709/140‑1 (MQ=255)
                                                                        tAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTggcgggg                                                               <  2:2142881/140‑1 (MQ=255)
                                                                        tAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTGCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTggcgggg                                                               <  2:287695/140‑1 (MQ=255)
                                                                          aTACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTggcggggcg                                                             >  2:832038/1‑140 (MQ=255)
                                                                             ccaccaCGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCggtg                                                          <  1:1236010/140‑1 (MQ=255)
                                                                             ccaccaCGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCggtg                                                          <  1:410277/140‑1 (MQ=255)
                                                                             acaccacGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCggtg                                                          <  2:643716/139‑1 (MQ=255)
                                                                               accacGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTggcggggcgggtgt                                                        >  2:917424/1‑136 (MQ=255)
                                                                               accacGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCggtggt                                                        <  1:579835/140‑1 (MQ=255)
                                                                               accacGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCgggggt                                                        >  2:1953837/1‑140 (MQ=255)
                                                                                 cacGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGCCCTGGCGATTGGCGGGGCGGtggtgg                                                      >  2:1944090/1‑140 (MQ=255)
                                                                                  acGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTAGCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTggg                                                     >  2:1808746/1‑140 (MQ=255)
                                                                                  acGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGGGGTGGTggg                                                     >  1:1674070/1‑140 (MQ=255)
                                                                                  acGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTggg                                                     >  1:478063/1‑140 (MQ=255)
                                                                                   cgccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGGGGTGGGc                                                    >  2:20274/1‑140 (MQ=255)
                                                                                    gccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCt                                                   <  2:1313017/140‑1 (MQ=255)
                                                                                     ccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGgggggggggtt                                                  >  2:226486/1‑137 (MQ=255)
                                                                                     ccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCt                                                   <  1:1364902/139‑1 (MQ=255)
                                                                                     ccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCt                                                   >  2:1364902/1‑139 (MQ=255)
                                                                                     ccgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTg                                                  <  1:23596/140‑1 (MQ=255)
                                                                                      cgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTgg                                                 >  2:61223/1‑140 (MQ=255)
                                                                                      cgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTgg                                                 >  1:1789082/1‑140 (MQ=255)
                                                                                      cgcTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAACCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTgg                                                 >  1:1721004/1‑140 (MQ=255)
                                                                                        cTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGAt                                               >  1:262687/1‑140 (MQ=255)
                                                                                              tGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGc                                         <  1:91056/140‑1 (MQ=255)
                                                                                               ggCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCg                                        <  1:229627/140‑1 (MQ=255)
                                                                                               ggCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCg                                        <  1:924145/140‑1 (MQ=255)
                                                                                                gCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGt                                       >  1:726642/1‑140 (MQ=255)
                                                                                                  tGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAACCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTAc                                     <  2:1721004/140‑1 (MQ=255)
                                                                                                       gCTTCTGACGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCaa                                <  1:61223/140‑1 (MQ=255)
                                                                                                              aCGTGGTGGACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTcc                         <  1:1662990/140‑1 (MQ=255)
                                                                                                                 tggtggACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCCGTGGTGGGGGGGGTTCTGGCCTACGTCCACTATTTCGcc                      >  2:1986847/1‑140 (MQ=255)
                                                                                                                  ggtggACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCca                     >  2:292189/1‑140 (MQ=255)
                                                                                                                   gtggACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGGGGTGGGCTGGGTCCTGGCGTACGTCAACTATTCCGCcag                    >  2:1680690/1‑140 (MQ=255)
                                                                                                                      gACTACGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGGACGTCAACTATTCCGCccgcag                 >  2:1967395/1‑140 (MQ=255)
                                                                                                                           cGAGGAGAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGTGCGGGGGCGGGCTGGATCCTTGCGCATGTCAAATTTTTCGCCctcagcagct            >  2:648348/1‑139 (MQ=255)
                                                                                                                              ggagAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGCAGATTGGCCTGGCGATTGGCGGGGCGGGGGTGGGCTGGGTGCTGGCGCCCGTCAAATTTTGCGCCAGCAGCGGCGTGc         >  2:1812760/1‑140 (MQ=255)
                                                                                                                              ggagAGCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGc         >  2:1876330/1‑140 (MQ=255)
                                                                                                                                agagCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAg       >  1:351410/1‑140 (MQ=255)
                                                                                                                                agagCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGGGGGCTGGGTCCTTGCGGACCTCAAATATTCCGCCAGCAGCAGCgcggag       >  2:2122578/1‑135 (MQ=255)
                                                                                                                                  agCCGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc     >  1:383610/1‑140 (MQ=255)
                                                                                                                                    ccGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCgg   <  2:1233686/140‑1 (MQ=255)
                                                                                                                                     cGCATCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGCGGTGGGCTGGAACCTGGGGTACGTCAACTATTTCGCCAGCAGCAGCGTGGAGGCGGt  >  2:433541/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGT  >  NC_000913/286315‑286587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A