Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	340,879	G→T	E239* (GAA→TAA)	yahI →	carbamate kinase‑like protein YahI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	340,879	0	G	T	99.1%	383.5 / ‑3.7	111	coding (715/951 nt)	yahI	carbamate kinase‑like protein YahI
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (61/49); minor base . (1/0); total (62/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

Only 100 of 112 total aligned reads displayed.

GCGGCGGTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCGAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAC  >  NC_000913/340742‑341015

gcggcgGTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTaa                                                                                                                                        <  1:539437/140‑1 (MQ=255)

 cggcggTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTaaa                                                                                                                                       <  1:1879703/140‑1 (MQ=255)

   gcggTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTaaaaa                                                                                                                                     <  2:1299917/140‑1 (MQ=255)

    cggTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAg                                                                                                                                    <  1:521740/140‑1 (MQ=255)

    cggTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAg                                                                                                                                    <  1:63871/140‑1 (MQ=255)

    cggTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAg                                                                                                                                    <  1:1954615/140‑1 (MQ=255)

      gTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAgtg                                                                                                                                  >  1:1429439/1‑140 (MQ=255)

          aaTTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTa                                                                                                                              <  1:1908545/140‑1 (MQ=255)

            ttCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTAtt                                                                                                                            <  1:1732145/140‑1 (MQ=255)

                 gTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACtt                                                                                                                       >  1:263177/1‑140 (MQ=255)

                    gTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTgg                                                                                                                    >  2:1323240/1‑140 (MQ=255)

                     tGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGc                                                                                                                   >  1:170514/1‑140 (MQ=255)

                     tGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGc                                                                                                                   >  1:937551/1‑140 (MQ=255)

                      gCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCa                                                                                                                  <  1:788150/140‑1 (MQ=255)

                       cGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCaa                                                                                                                 >  1:1003837/1‑140 (MQ=255)

                          aCTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAAcc                                                                                                              <  2:1449694/140‑1 (MQ=255)

                          aCTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAAcc                                                                                                              <  2:1881647/140‑1 (MQ=255)

                              aCGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCgcag                                                                                                          >  1:2097714/1‑140 (MQ=255)

                              aCGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCgcag                                                                                                          >  1:1853421/1‑140 (MQ=255)

                                  gggAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCgcagcagc                                                                                                      <  1:1935730/140‑1 (MQ=255)

                                  gggAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCgcagcagc                                                                                                      <  2:937551/140‑1 (MQ=255)

                                      gaTTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGgc                                                                                                  <  1:1323240/140‑1 (MQ=255)

                                      gaTTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGgc                                                                                                  <  2:170514/140‑1 (MQ=255)

                                       aTTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGgcg                                                                                                 <  2:1003837/140‑1 (MQ=255)

                                         tACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCt                                                                                               >  1:1052584/1‑140 (MQ=255)

                                             aaaGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGAt                                                                                           <  1:69275/140‑1 (MQ=255)

                                             aaaGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGAt                                                                                           <  2:263177/140‑1 (MQ=255)

                                                 cGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCggg                                                                                       <  1:222002/140‑1 (MQ=255)

                                                 cGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCggg                                                                                       >  1:499444/1‑140 (MQ=255)

                                                 cGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCggg                                                                                       >  1:1506052/1‑140 (MQ=255)

                                                   tGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTg                                                                                     >  2:208382/1‑140 (MQ=255)

                                                   tGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTg                                                                                     >  2:51862/1‑140 (MQ=255)

                                                   tGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTg                                                                                     >  1:1185935/1‑140 (MQ=255)

                                                     gACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGa                                                                                   <  2:1643883/140‑1 (MQ=255)

                                                          ggTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTg                                                                              >  1:749678/1‑140 (MQ=255)

                                                           gTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGc                                                                             >  1:1963634/1‑140 (MQ=255)

                                                           gTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGc                                                                             >  1:1715819/1‑140 (MQ=255)

                                                           gTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGc                                                                             >  2:834688/1‑140 (MQ=255)

                                                               tCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGccacc                                                                         >  2:1424481/1‑140 (MQ=255)

                                                                cGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGccacca                                                                        <  1:1200168/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  1:1416458/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  1:1424481/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  1:1888744/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  2:1079629/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  2:9034/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  2:749678/140‑1 (MQ=255)

                                                                    aaaGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  2:1506052/140‑1 (MQ=255)

                                                                      aGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAccc                                                                  <  1:51862/140‑1 (MQ=255)

                                                                       gATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCg                                                                 <  2:1309238/140‑1 (MQ=255)

                                                                       aaTCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCg                                                                 <  2:1715819/139‑1 (MQ=255)

                                                                        aTCTCTCTACCGCGCTGCTGGCCAGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCATCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGGTTGATCGAGTGGATATTGCCACCATGACCCGc                                                                <  2:378193/140‑1 (MQ=255)

                                                                         tctctcTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCt                                                               <  2:1052584/140‑1 (MQ=255)

                                                                             tctACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTatat                                                           <  2:600819/140‑1 (MQ=255)

                                                                             tctACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTatat                                                           >  1:1342465/1‑140 (MQ=255)

                                                                                  cgcgCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAgg                                                      >  2:152742/1‑140 (MQ=255)

                                                                                  cgcgCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAgg                                                      >  1:229670/1‑140 (MQ=255)

                                                                                    cgcTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGaa                                                    >  1:12119/1‑140 (MQ=255)

                                                                                    cgcTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGaa                                                    >  2:1975705/1‑140 (MQ=255)

                                                                                    cgcTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGaa                                                    >  1:1098520/1‑140 (MQ=255)

                                                                                    cgcTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGaa                                                    >  1:1085577/1‑140 (MQ=255)

                                                                                      ctgctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    <  1:1828634/122‑1 (MQ=255)

                                                                                      ctgctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGAc                                                                    >  2:1828634/1‑122 (MQ=255)

                                                                                      ctgctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAgg                                                  >  1:2005455/1‑140 (MQ=255)

                                                                                       tgctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAggg                                                 >  1:2119718/1‑140 (MQ=255)

                                                                                         ctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCa                                               >  2:1163809/1‑140 (MQ=255)

                                                                                         ctgGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCa                                               >  1:2110355/1‑140 (MQ=255)

                                                                                           ggCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCAtt                                             >  1:1056584/1‑140 (MQ=255)

                                                                                            gCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCAttt                                            >  2:798324/1‑140 (MQ=255)

                                                                                              ccGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcc                                          <  1:152742/140‑1 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       <  1:208382/140‑1 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  2:2051575/1‑140 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  2:917716/1‑140 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  2:1602019/1‑140 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  2:465267/1‑140 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  1:1710941/1‑140 (MQ=255)

                                                                                                 tGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgc                                       >  2:1195102/1‑140 (MQ=255)

                                                                                                  gAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTcccgcc                                      <  2:1963634/140‑1 (MQ=255)

                                                                                                           gCCGACATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCAt                             >  2:1006138/1‑140 (MQ=255)

                                                                                                                cATTCTTGTGATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGc                        >  2:1917583/1‑140 (MQ=255)

                                                                                                                         gATCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAatca               >  2:2072273/1‑140 (MQ=255)

                                                                                                                          aTCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAatcat              >  1:892261/1‑140 (MQ=255)

                                                                                                                           tCACCACTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAatcatc             >  2:2068748/1‑140 (MQ=255)

                                                                                                                            caccacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCg            <  2:1085577/140‑1 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  1:8450/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  2:1925771/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  2:132327/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  1:1914954/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  1:1779311/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  1:1029031/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTCTAAAAAGTGTGCATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGc           >  2:885577/1‑140 (MQ=255)

                                                                                                                             accacTGGCGTC‑TAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGcc          >  2:735488/1‑140 (MQ=255)

                                                                                                                               cacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCa         >  2:194995/1‑140 (MQ=255)

                                                                                                                               cacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCa         >  2:684669/1‑140 (MQ=255)

                                                                                                                               cacTGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCa         >  2:819105/1‑140 (MQ=255)

                                                                                                                                  tGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGcc      >  1:872696/1‑140 (MQ=255)

                                                                                                                                  tGGCGTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGcc      >  1:1593344/1‑140 (MQ=255)

                                                                                                                                      gTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAc  <  1:1585567/140‑1 (MQ=255)

                                                                                                                                      gTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAc  <  2:892261/140‑1 (MQ=255)

                                                                                                                                      gTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAc  <  1:1194412/140‑1 (MQ=255)

                                                                                                                                      gTCTAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAc  <  1:1006138/140‑1 (MQ=255)

GCGGCGGTGGAATTCCGGTAGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTATCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTTGTGATCACCACTGGCGTCGAAAAAGTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATATTGCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGAC  >  NC_000913/340742‑341015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A