Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,810	C→A	P369T (CCG→ACG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,810	0	C	A	100.0%	376.1 / NA	111	P369T (CCG→ACG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (58/53); total (58/53)

Only 100 of 119 total aligned reads displayed.

AGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGG  >  NC_000913/353673‑353943

agaAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACg                                                                                                                                     >  1:799708/1‑140 (MQ=255)

  aaaTATCAGGTTAGCCGGATGTTTTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAACCACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAgacgac                                                                                                                                   <  1:2059134/140‑1 (MQ=255)

  aaaTATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAgacgac                                                                                                                                   <  1:172058/140‑1 (MQ=255)

    atatCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCg                                                                                                                                 >  2:809370/1‑140 (MQ=255)

       tCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCT‑GAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAg                                                                                                                             <  2:1696927/140‑1 (MQ=255)

         aGGTTAGCCGGATTTTCCCAGCGCCGCCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGt                                                                                                                            <  2:799708/140‑1 (MQ=255)

         aGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGt                                                                                                                            <  2:1267589/140‑1 (MQ=255)

          ggTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGtt                                                                                                                           >  1:919134/1‑140 (MQ=255)

            ttAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTgg                                                                                                                         >  1:1773280/1‑140 (MQ=255)

              aGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGt                                                                                                                       <  1:288051/140‑1 (MQ=255)

              aGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGt                                                                                                                       <  1:2061289/140‑1 (MQ=255)

              aGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGt                                                                                                                       <  1:809370/140‑1 (MQ=255)

               gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGGTCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTg                                                                                                                      >  2:1971895/1‑140 (MQ=255)

               gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCa                                                                                                                              <  1:1087622/132‑1 (MQ=255)

               gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTg                                                                                                                      >  2:1756359/1‑140 (MQ=255)

               gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTg                                                                                                                      >  1:233281/1‑140 (MQ=255)

               gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTg                                                                                                                      >  2:1141554/1‑140 (MQ=255)

                ccGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGa                                                                                                                     >  1:1690926/1‑140 (MQ=255)

                ccGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGa                                                                                                                     >  2:1130230/1‑140 (MQ=255)

                ccGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGa                                                                                                                     >  2:1985951/1‑140 (MQ=255)

                 cGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAg                                                                                                                    <  1:1652131/140‑1 (MQ=255)

                    aTGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCaa                                                                                                                 <  1:1399155/140‑1 (MQ=255)

                     tGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAAt                                                                                                                >  2:1088836/1‑140 (MQ=255)

                     tGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAAt                                                                                                                >  1:1257991/1‑140 (MQ=255)

                     tGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAAt                                                                                                                >  1:1945772/1‑140 (MQ=255)

                        tctcAGCGCGGCCGGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACg                                                                                                             <  1:109238/140‑1 (MQ=255)

                        tctcAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACg                                                                                                             <  2:1707302/140‑1 (MQ=255)

                        tctcAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACg                                                                                                             <  1:298207/140‑1 (MQ=255)

                            aGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTgg                                                                                                         <  2:627139/140‑1 (MQ=255)

                            aGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTgg                                                                                                         <  1:1756359/140‑1 (MQ=255)

                              cgcCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGAt                                                                                                       >  2:222516/1‑140 (MQ=255)

                                 cGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGAtgtg                                                                                                    >  2:1418282/1‑140 (MQ=255)

                                  gACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGc                                                                                                   <  1:1147298/140‑1 (MQ=255)

                                  gACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGc                                                                                                   <  1:1985951/140‑1 (MQ=255)

                                   aCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGcc                                                                                                  >  1:61440/1‑140 (MQ=255)

                                   aCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGcc                                                                                                  >  1:292979/1‑140 (MQ=255)

                                   aCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGcc                                                                                                  >  1:323100/1‑140 (MQ=255)

                                        cATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCa                                                                                             <  2:233281/140‑1 (MQ=255)

                                        cATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCa                                                                                             <  1:1130230/140‑1 (MQ=255)

                                           tCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCg                                                                                          <  1:643473/140‑1 (MQ=255)

                                            cgcgTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGa                                                                                         <  1:487511/140‑1 (MQ=255)

                                            cgcgTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGa                                                                                         <  1:217022/140‑1 (MQ=255)

                                             gcgTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGCGAACCGCTGGACGAGACGACCGCCAGTTGGGCGAGAAATACGCTGGATGTGCCGGTCACCGAc                                                                                        >  2:698935/1‑140 (MQ=255)

                                             gcgTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGAc                                                                                        <  1:1418282/140‑1 (MQ=255)

                                              cgTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACa                                                                                       >  2:1162606/1‑140 (MQ=255)

                                                  cTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATTCGCTGGATGTGCCGGTCATCGACAacta                                                                                   >  2:2024770/1‑140 (MQ=255)

                                                  cTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAacta                                                                                   <  1:702840/140‑1 (MQ=255)

                                                  cTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAacta                                                                                   <  1:953677/140‑1 (MQ=255)

                                                          aTTCCCTGCCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGa                                                                           <  1:1381606/140‑1 (MQ=255)

                                                            tCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGAcc                                                                         >  1:816838/1‑140 (MQ=255)

                                                                     cTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGc                                                                >  1:1305697/1‑140 (MQ=255)

                                                                         aaTTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                                                            <  1:989351/140‑1 (MQ=255)

                                                                         aaTTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                                                            <  1:1162606/140‑1 (MQ=255)

                                                                             cGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGAt                                                        >  1:599565/1‑140 (MQ=255)

                                                                                              gtcgCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg                                       >  1:1516664/1‑140 (MQ=255)

                                                                                              gtcgCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg                                       <  2:1639551/140‑1 (MQ=255)

                                                                                              gtcgCTGGAAGTGCTCTATCGGGCTGGAGATCCGCTGGACGAGACGCCCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg                                       <  1:1170798/140‑1 (MQ=255)

                                                                                              atcgCTGGAAGTGCTGTATCTGTCTGATGATCATCTGGACGATACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg                                       <  2:61440/139‑1 (MQ=255)

                                                                                                cgCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGt                                     >  2:581500/1‑140 (MQ=255)

                                                                                                cgCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGt                                     >  1:417025/1‑140 (MQ=255)

                                                                                                  cTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCt                                   >  2:670057/1‑140 (MQ=255)

                                                                                                  cTGGAAGTGCTCTATCTGGCTGGAGAACCGCCGGACGAGACGACCGCCAGTTGGGAGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCt                                   >  2:131481/1‑140 (MQ=255)

                                                                                                   tGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTg                                  >  2:529492/1‑140 (MQ=255)

                                                                                                   tGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTg                                  <  2:1480543/140‑1 (MQ=255)

                                                                                                   tGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTg                                  <  2:919134/140‑1 (MQ=255)

                                                                                                      aaGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGgac                               >  1:1114028/1‑140 (MQ=255)

                                                                                                      aaGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGgac                               >  1:588803/1‑140 (MQ=255)

                                                                                                        gTGCTCTATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGgacga                             >  1:190997/1‑140 (MQ=255)

                                                                                                             ctATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGc                        >  2:1878810/1‑140 (MQ=255)

                                                                                                             ctATCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCAGGACGACAGGc                        >  2:1311257/1‑140 (MQ=255)

                                                                                                               aTCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCg                      >  2:683745/1‑140 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  2:599565/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  2:417025/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  2:816838/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  2:1988102/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  1:1878810/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  1:713581/140‑1 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     >  2:1623720/1‑140 (MQ=255)

                                                                                                                tCTGGCTGGAGAACCGCTGGACGAGACGACCACCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGa                     <  1:529492/140‑1 (MQ=255)

                                                                                                                 ctggctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGAc                    <  2:1516664/140‑1 (MQ=255)

                                                                                                                    gctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGAcgcg                 >  1:967496/1‑140 (MQ=255)

                                                                                                                     gtggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGt                <  2:94553/139‑1 (MQ=255)

                                                                                                                     ctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTTATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGt                >  1:2111324/1‑140 (MQ=255)

                                                                                                                     ctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGt                >  2:1409699/1‑140 (MQ=255)

                                                                                                                     ctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGt                <  2:1305697/140‑1 (MQ=255)

                                                                                                                     ctggAGAACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGt                >  2:1122358/1‑140 (MQ=255)

                                                                                                                        gagaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTg             >  2:369499/1‑140 (MQ=255)

                                                                                                                        gagaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTg             >  2:575608/1‑140 (MQ=255)

                                                                                                                          gaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTggg           <  1:1070037/140‑1 (MQ=255)

                                                                                                                          gaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTggg           >  1:1487794/1‑140 (MQ=255)

                                                                                                                          gaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTggg           >  2:1260444/1‑140 (MQ=255)

                                                                                                                          gaACCGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTggg           >  2:1007539/1‑140 (MQ=255)

                                                                                                                          aaaCCGCTGGACGAGAGGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTggg           <  2:1523798/139‑1 (MQ=255)

                                                                                                                              cGCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGc       >  1:1011996/1‑140 (MQ=255)

                                                                                                                               gCTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGcc      >  2:2488/1‑140 (MQ=255)

                                                                                                                                cTGGACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGccc     <  2:292979/140‑1 (MQ=255)

                                                                                                                                  ggACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATTGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCg   >  1:1710268/1‑140 (MQ=255)

                                                                                                                                  ggACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCg   <  2:1257991/140‑1 (MQ=255)

                                                                                                                                  ggACGAGACGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCg   <  2:588803/140‑1 (MQ=255)

                                                                                                                                   gacgagacgaCCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCgg  >  1:1931611/1‑140 (MQ=255)

AGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGG  >  NC_000913/353673‑353943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A