Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,437	G→T	H623Q (CAC→CAA)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,437	0	G	T	100.0%	313.4 / NA	90	H623Q (CAC→CAA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (49/41); total (49/41)

TTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATA  >  NC_000913/364302‑364571

ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCATTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTgttgtt                                                                                                                                     >  1:2101001/1‑140 (MQ=255)

ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTAGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTgttgtt                                                                                                                                     <  1:848135/140‑1 (MQ=255)

 tCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGttt                                                                                                                                    >  1:1731606/1‑140 (MQ=255)

     cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCt                                                                                                                                <  1:623327/140‑1 (MQ=255)

     cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCt                                                                                                                                <  2:1548562/140‑1 (MQ=255)

          gCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGt                                                                                                                           >  1:1847535/1‑140 (MQ=255)

          gCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGt                                                                                                                           >  2:2645/1‑140 (MQ=255)

            cAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCa                                                                                                                         <  1:727846/140‑1 (MQ=255)

               cGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAgcg                                                                                                                      >  2:1492130/1‑140 (MQ=255)

                ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAgcgc                                                                                                                     <  1:2645/140‑1 (MQ=255)

                         tCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATgcgg                                                                                                            >  1:1672006/1‑140 (MQ=255)

                         tCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATgcgg                                                                                                            >  1:1667004/1‑140 (MQ=255)

                            aGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGt                                                                                                         >  1:187442/1‑140 (MQ=255)

                              cgcCACCATCCAGTGCGGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGc                                                                                                       >  1:1114154/1‑140 (MQ=255)

                              cgcCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGc                                                                                                       >  1:11124/1‑140 (MQ=255)

                              cgcCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGc                                                                                                       >  2:2087622/1‑140 (MQ=255)

                              cgcCACCATCCAGTGCAGGAGCTCGTTATCGATATGACGGAACAGGTATTCGCTGGTCTCTTCGATGGTTTGCCCGGATAATCGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGc                                                                                                       <  2:1928770/140‑1 (MQ=255)

                                ccaccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCgg                                                                                                     >  1:131742/1‑140 (MQ=255)

                                 caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                                                                                    <  2:580627/140‑1 (MQ=255)

                                 caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                                                                                    <  1:1734469/140‑1 (MQ=255)

                                 caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                                                                                    <  2:1667004/140‑1 (MQ=255)

                                 caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                                                                                    >  1:204003/1‑140 (MQ=255)

                                     aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCATTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGc                                                                                                <  2:2101001/140‑1 (MQ=255)

                                     aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGACAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGc                                                                                                <  1:1457961/140‑1 (MQ=255)

                                     aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGc                                                                                                <  1:2087622/140‑1 (MQ=255)

                                     aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGc                                                                                                <  1:372504/140‑1 (MQ=255)

                                     aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCAGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGc                                                                                                <  2:267894/140‑1 (MQ=255)

                                      tCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCa                                                                                               <  2:187442/140‑1 (MQ=255)

                                        cAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCaaa                                                                                             <  1:442849/140‑1 (MQ=255)

                                           tGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagac                                                                                          >  1:1446369/1‑140 (MQ=255)

                                           tGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagac                                                                                          >  1:1907366/1‑140 (MQ=255)

                                             cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagacca                                                                                        >  1:347760/1‑140 (MQ=255)

                                             cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagacca                                                                                        >  1:1025608/1‑140 (MQ=255)

                                             cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagacca                                                                                        >  1:1473784/1‑140 (MQ=255)

                                              aGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       <  2:1731606/140‑1 (MQ=255)

                                              aGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       <  2:1272402/140‑1 (MQ=255)

                                              aGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       <  1:144016/140‑1 (MQ=255)

                                              aGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       <  2:585509/140‑1 (MQ=255)

                                               ggAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccaga                                                                                      <  2:1473784/140‑1 (MQ=255)

                                               ggAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccaga                                                                                      <  1:2119249/140‑1 (MQ=255)

                                                gAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       <  2:1482129/138‑1 (MQ=255)

                                                gAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                                                                                       >  1:1482129/1‑138 (MQ=255)

                                                gAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCAATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccagac                                                                                     <  1:1313380/140‑1 (MQ=255)

                                                  gCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCg                                                                                   <  1:610047/140‑1 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  >  2:149670/1‑140 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  <  2:137780/140‑1 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  <  2:204003/140‑1 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  <  2:337231/140‑1 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  <  2:873762/140‑1 (MQ=255)

                                                   cTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                                                                                  <  1:1492130/140‑1 (MQ=255)

                                                     cGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTc                                                                                >  1:649726/1‑140 (MQ=255)

                                                     cGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTc                                                                                >  2:1923903/1‑140 (MQ=255)

                                                     cGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTc                                                                                >  2:2100433/1‑140 (MQ=255)

                                                      gTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCa                                                                               <  1:399513/140‑1 (MQ=255)

                                                      gTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCa                                                                               <  2:348678/140‑1 (MQ=255)

                                                        tATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATa                                                                             <  1:1581110/140‑1 (MQ=255)

                                                                  aCGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGc                                                                   <  1:20685/140‑1 (MQ=255)

                                                                   cGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCg                                                                  <  1:193043/140‑1 (MQ=255)

                                                                   aggAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCg                                                                  <  2:1672006/139‑1 (MQ=255)

                                                                   aggAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCg                                                                  <  2:31483/139‑1 (MQ=255)

                                                                    ggAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGa                                                                 <  2:347760/140‑1 (MQ=255)

                                                                      aaCAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCa                                                                               <  1:1504472/124‑1 (MQ=255)

                                                                      aaCAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATc                                                               <  1:1923903/140‑1 (MQ=255)

                                                                      aaCAGGTATTCGCTGGTCACTTCGATAGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCa                                                                               >  2:1504472/1‑124 (MQ=255)

                                                                        cAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGt                                                             >  1:1644014/1‑140 (MQ=255)

                                                                           gTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCg                                                          >  1:657562/1‑140 (MQ=255)

                                                                                cGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTa                                                     >  2:1237530/1‑140 (MQ=255)

                                                                                cGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTa                                                     >  1:12038/1‑140 (MQ=255)

                                                                                   tGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑ct                                                 >  2:659823/1‑139 (MQ=255)

                                                                                     gTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGcc                                               >  1:1318773/1‑140 (MQ=255)

                                                                                     gTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGcc                                               >  1:944738/1‑140 (MQ=255)

                                                                                     gTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGcc                                               <  2:1907366/140‑1 (MQ=255)

                                                                                     gTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCGGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGGCCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGcc                                               >  1:1919018/1‑140 (MQ=255)

                                                                                      tCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCa                                              >  1:1157640/1‑140 (MQ=255)

                                                                                          ttCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAAt                                          >  1:1082730/1‑140 (MQ=255)

                                                                                          ttCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAAt                                          >  2:1518404/1‑140 (MQ=255)

                                                                                          ttCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAAt                                          >  1:2043956/1‑140 (MQ=255)

                                                                                               tGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCAccg                                     >  1:1774011/1‑140 (MQ=255)

                                                                                               tGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCAccg                                     >  2:373349/1‑140 (MQ=255)

                                                                                                gttttGCCCGGAAAAACGGAACTGAAAAACCTGCTGTTGTTGTTTTGCTTCCGTCAGCGCTGGATGGGGCGTGCGGTCGAAAAAGACCAGACCGTTAATAAAGACCGGGCGTTCGTTCGGCGTAT‑CGCAAAAATCAccgc                                    <  2:1446369/138‑1 (MQ=255)

                                                                                                 gTTTGCCCGGAAAAACGGACCTGAAAAACCTGCTGTTGTTGTTTTGCTTCCGTCAGCGCTGGAGGGGGCGTGCGGTCGGAAAAGCCCAGACCGTTCATACAGACCGGGCGATCGTTCGGCGTAT‑CGCAAAAATCAccgcc                                   <  2:649726/140‑1 (MQ=255)

                                                                                                 gTTGGCCCGAAAAAACGGAACTGGAAAAACGGCTGCTGTTGTTTTGCTTCCGTCAGCGCGGGATGGGGCGTGGGGTCGGAAAAGACCAGCCCGTTCATACAGAACGGGCGATCGTTCGGCGTAT‑CGCAAAAAACAccgcc                                   <  2:1114154/140‑1 (MQ=255)

                                                                                                    tGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTa                                >  1:1781257/1‑140 (MQ=255)

                                                                                                    tGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTa                                >  1:138241/1‑140 (MQ=255)

                                                                                                    tGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTa                                >  2:1265400/1‑140 (MQ=255)

                                                                                                           aTAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGAc                         >  2:1943145/1‑140 (MQ=255)

                                                                                                            tAAACGGAACTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGAcc                        >  1:563435/1‑140 (MQ=255)

                                                                                                                    agtgaaaaacCTGCTGTTTTTGTTTTGCTCCCGTCAGCGCTGGGGGCGGGGTGGGGTCGGCAAAGACCAGCCCGTTCAAACAAACCTGGCGATGGTTGGGCTTAC‑CCCAAAAATCACCGCCGTAAGCCGACCACGGGTTg                <  2:563435/135‑1 (MQ=255)

                                                                                                                    aCTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTg                <  2:1082730/140‑1 (MQ=255)

                                                                                                                     cTGGAAAAACTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTTATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGc               >  1:127038/1‑140 (MQ=255)

                                                                                                                             aCTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtca       >  1:2027559/1‑140 (MQ=255)

                                                                                                                             aCTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtca       >  2:490593/1‑140 (MQ=255)

                                                                                                                             aCTGCTGCTGTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtca       >  1:485412/1‑140 (MQ=255)

                                                                                                                              gtgctgctgTTGTTTTGCTTCAGACAGGGTGGGATGCGGCGTGCGGTCGAAGACAACAAGACCATTCATGAAAAACGGGCTTTCGTT‑GCCGTTTACGCAAAATTCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtcat      <  2:11124/139‑1 (MQ=255)

                                                                                                                              ctgctgctgTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCACCGTAAGCCGACCACGGGTTGCCGTTTtcat      <  1:2100433/140‑1 (MQ=255)

                                                                                                                              ctgcggctgTTTTTTTGCTCCCGTCAGCGCTGGATGGGGGGTGCGGTCGGAAAAGACAAGACCGTTCAAACAGAACGGGCGATCTTTCGGCGTAT‑CGCAAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtcat      <  2:1157640/140‑1 (MQ=255)

                                                                                                                                gctgctgTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTtcatca    >  1:1754151/1‑140 (MQ=255)

                                                                                                                                  tgctgTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTcatcatt  >  2:1725197/1‑139 (MQ=255)

                                                                                                                                  tgctgTTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCata  >  2:1507222/1‑140 (MQ=255)

TTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTAT‑CGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATA  >  NC_000913/364302‑364571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A