Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 515,074 G→T R234S (CGC→AGC)  qmcA ← PHB domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913515,0740GT100.0% 371.1 / NA 107R234S (CGC→AGC) qmcAPHB domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (74/33);  total (74/33)

Only 100 of 111 total aligned reads displayed.

GCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCGGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCGC  >  NC_000913/514942‑515209
                                                                                                                                    |                                                                                                                                       
  atcatcACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGc                                                                                                                                >  1:1213583/1‑140 (MQ=255)
     atcaCTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCGCCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTc                                                                                                                             >  2:1997906/1‑140 (MQ=255)
       caCTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTcgg                                                                                                                           >  2:290784/1‑140 (MQ=255)
          tactTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTcggcgg                                                                                                                        >  2:291425/1‑140 (MQ=255)
            atttGCTGTTACTGGAGGATCCGATCTGCTGTAACGATTCGGTGTATTTCTGCGCTACGAAGTAGGTCACCGACTGAATATCACCGGAGGCGATAGCTTAAGACGCCATTTTGGTGGAGATGGCTTTTGCTTCGGCGGat                                                                                                                      <  2:1877250/139‑2 (MQ=255)
               tGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGt                                                                                                                   >  1:1120229/1‑140 (MQ=255)
               tGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGt                                                                                                                   >  2:1121890/1‑140 (MQ=255)
               tGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGt                                                                                                                   >  1:1747058/1‑140 (MQ=255)
               tGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGt                                                                                                                   >  2:2105818/1‑140 (MQ=255)
               tGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGt                                                                                                                   >  1:740012/1‑140 (MQ=255)
                 cTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTGTGGCGGCGCTGGCTTCTGCTTCGGCGGAACGTTc                                                                                                                 >  2:1093342/1‑140 (MQ=255)
                      aCTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCAcgcg                                                                                                            >  1:1618417/1‑140 (MQ=255)
                      aCTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCAcgcg                                                                                                            >  2:1957802/1‑140 (MQ=255)
                      aCTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCAc                                                                                                               <  1:1404927/137‑1 (MQ=255)
                         ggaggaACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCtt                                                                                                         <  1:2105818/140‑1 (MQ=255)
                         ggaggaACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCtt                                                                                                         <  2:1747058/140‑1 (MQ=255)
                         ggaggaACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCtt                                                                                                         <  2:740012/140‑1 (MQ=255)
                         ggaggaACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCtt                                                                                                         <  2:1287264/140‑1 (MQ=255)
                           aggaACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCa                                                                                                       >  1:1324120/1‑140 (MQ=255)
                              aaCCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGcc                                                                                                    >  1:259611/1‑140 (MQ=255)
                              aaCCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGcc                                                                                                    >  1:220931/1‑140 (MQ=255)
                               aCCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGACGGAACGTTCACGCGCTTCAGCCt                                                                                                   >  2:1455688/1‑140 (MQ=255)
                                 cGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGt                                                                                                 >  2:304164/1‑140 (MQ=255)
                                 cGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGt                                                                                                 >  1:127378/1‑140 (MQ=255)
                                  gATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTa                                                                                                >  1:1820565/1‑140 (MQ=255)
                                  gATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTa                                                                                                >  1:1867675/1‑140 (MQ=255)
                                    tCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTaaa                                                                                              <  2:220931/140‑1 (MQ=255)
                                        ctgTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACg                                                                                          >  2:1013065/1‑140 (MQ=255)
                                        ctgTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACg                                                                                          <  2:259611/140‑1 (MQ=255)
                                           tAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCg                                                                                       >  1:2122584/1‑140 (MQ=255)
                                             aCGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGAc                                                                                     <  1:1013065/140‑1 (MQ=255)
                                              cGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACt                                                                                    >  2:150699/1‑140 (MQ=255)
                                              cGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACt                                                                                    >  1:2086590/1‑140 (MQ=255)
                                                  tCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACg                                                                                >  1:1139274/1‑140 (MQ=255)
                                                   cGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGt                                                                               >  2:241020/1‑140 (MQ=255)
                                                   cGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGt                                                                               >  1:677232/1‑140 (MQ=255)
                                                     gtgtATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTc                                                                             >  1:1119848/1‑140 (MQ=255)
                                                           ttCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACAACATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTc                                                                       <  2:1618417/140‑1 (MQ=255)
                                                                cgcTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTTGCCTTCCGCtt                                                                  >  1:716645/1‑140 (MQ=255)
                                                                cgcTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCtt                                                                  >  2:1412794/1‑140 (MQ=255)
                                                                cgcTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCtt                                                                  >  1:1394793/1‑140 (MQ=255)
                                                                     cGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAgg                                                             >  1:1480351/1‑140 (MQ=255)
                                                                     cGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAgg                                                             >  1:1147012/1‑140 (MQ=255)
                                                                     cGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAgg                                                             >  1:1152614/1‑140 (MQ=255)
                                                                          tagtTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGAtttt                                                        >  2:720224/1‑139 (MQ=255)
                                                                           agtTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGc                                                       <  1:304164/140‑1 (MQ=255)
                                                                             ttCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGa                                                     <  2:1286276/140‑1 (MQ=255)
                                                                              tCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGAc                                                    <  2:127378/140‑1 (MQ=255)
                                                                              tCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGAc                                                    <  1:1745954/140‑1 (MQ=255)
                                                                              tCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGAc                                                    <  1:1827030/140‑1 (MQ=255)
                                                                               cACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACt                                                   >  1:281590/1‑140 (MQ=255)
                                                                               cACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACt                                                   >  1:942443/1‑140 (MQ=255)
                                                                                  cGCCTGAATATCATCGGAGGCGATAGCTTCAGAGACCATTTTGGTGTCGCAGGCTTCTGCTTCGGCGTAACGTTCACGCGTTTCAGCCTGTAAAGACGCCGACTGACGTGCGCCTTCCCCTTTCAGGGTTTGCGACtagt                                                >  2:346478/1‑137 (MQ=255)
                                                                                  cGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGtt                                                >  1:1299155/1‑140 (MQ=255)
                                                                                  cGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGtt                                                >  2:102750/1‑140 (MQ=255)
                                                                                  cGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGtt                                                >  2:1632985/1‑140 (MQ=255)
                                                                                  cGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGtt                                                >  1:1642659/1‑140 (MQ=255)
                                                                                    ccTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGtttt                                              >  1:2138949/1‑140 (MQ=255)
                                                                                      tGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTc                                            <  2:2122584/140‑1 (MQ=255)
                                                                                       gAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTct                                           >  2:790368/1‑139 (MQ=255)
                                                                                       gAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCa                                           >  1:853689/1‑140 (MQ=255)
                                                                                           atCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCtt                                       <  1:315897/140‑1 (MQ=255)
                                                                                            tCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTc                                      >  1:932277/1‑140 (MQ=255)
                                                                                             cACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCg                                     <  2:1120229/140‑1 (MQ=255)
                                                                                             cACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCg                                     >  1:1379828/1‑140 (MQ=255)
                                                                                               ccGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGc                                   >  1:1583523/1‑140 (MQ=255)
                                                                                               ccGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGc                                   >  1:1642930/1‑140 (MQ=255)
                                                                                                cGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCt                                  >  2:1038755/1‑140 (MQ=255)
                                                                                                cGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCt                                  >  1:723450/1‑140 (MQ=255)
                                                                                                  gAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCttt                                >  1:1934653/1‑140 (MQ=255)
                                                                                                  gAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCttt                                >  1:2043958/1‑140 (MQ=255)
                                                                                                  gAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCttt                                >  2:733487/1‑140 (MQ=255)
                                                                                                  gAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCttt                                >  1:2147820/1‑140 (MQ=255)
                                                                                                  gAGGCGATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCttt                                >  1:1201766/1‑140 (MQ=255)
                                                                                                       gATAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAggt                           >  2:819059/1‑139 (MQ=255)
                                                                                                        aTAGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGAt                          >  2:636364/1‑140 (MQ=255)
                                                                                                          aGCTTCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGAttt                        >  2:221718/1‑140 (MQ=255)
                                                                                                              tCAGACAGCATTTTGGTGGCTCTGACTTATGCGTCGGTGGAACGATCACGCGCTTCAGCCTGGAAAGACGTCGCCTGACGATTGCTGGTGTCTTTCACGATGTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGc                    >  2:2019473/1‑140 (MQ=255)
                                                                                                              tCAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGc                    <  2:1119848/140‑1 (MQ=255)
                                                                                                               cAGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGcc                   >  2:586929/1‑140 (MQ=255)
                                                                                                                aGACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCt                  >  2:727338/1‑140 (MQ=255)
                                                                                                                 gACACCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTg                 >  1:1172535/1‑140 (MQ=255)
                                                                                                                  acacCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGa                <  2:716645/140‑1 (MQ=255)
                                                                                                                  acacCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGa                <  2:1642930/140‑1 (MQ=255)
                                                                                                                  acacCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGa                <  2:2147820/140‑1 (MQ=255)
                                                                                                                   cacCATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGAc               >  2:1541010/1‑140 (MQ=255)
                                                                                                                     ccATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACgg             <  2:1299155/140‑1 (MQ=255)
                                                                                                                      cATTTTGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGa            >  1:508511/1‑140 (MQ=255)
                                                                                                                         tttGGTGGCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATcc         >  1:1780692/1‑140 (MQ=255)
                                                                                                                           tggtggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCt       <  1:291425/140‑1 (MQ=255)
                                                                                                                           tggtggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCt       <  1:618073/140‑1 (MQ=255)
                                                                                                                           tggtggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCt       >  2:1589008/1‑140 (MQ=255)
                                                                                                                             gtggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTc     <  2:2043958/140‑1 (MQ=255)
                                                                                                                             gtggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTc     <  2:1480351/140‑1 (MQ=255)
                                                                                                                              tggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTcc    >  2:1249232/1‑140 (MQ=255)
                                                                                                                               ggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCg   <  1:425275/140‑1 (MQ=255)
                                                                                                                               ggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCg   <  1:720224/140‑1 (MQ=255)
                                                                                                                               ggCGCTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCg   <  1:819059/140‑1 (MQ=255)
                                                                                                                                gcgcTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCGc  >  2:1920117/1‑140 (MQ=255)
                                                                                                                                gcgcTGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCGc  >  1:1172955/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                       
GCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCACCGCCTGAATATCACCGGAGGCGATAGCTTCAGACACCATTTTGGTGGCGCGGGCTTCTGCTTCGGCGGAACGTTCACGCGCTTCAGCCTGTAAAAACGCCGACTGACGTTCGCCTTCCGCTTTCAGGATTTGCGACTGTTTTTCACCTTCGGCTTTGAGGATTTCCGCCTGACGGATCCCTTCCGC  >  NC_000913/514942‑515209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A