Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,093	G→T	G60C (GGT→TGT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,093	0	G	T	100.0%	430.0 / NA	126	G60C (GGT→TGT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (56/70); total (56/70)

Only 100 of 134 total aligned reads displayed.

TGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGG  >  NC_000913/533956‑534225

 ggAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGtt                                                                                                                                   >  2:1040413/1‑140 (MQ=255)

  gagaAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGGAGGTGCTTCGCACATGGCGGAATGTta                                                                                                                                  >  1:308221/1‑140 (MQ=255)

  gagaAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTta                                                                                                                                  >  2:2124712/1‑140 (MQ=255)

  gagaAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTta                                                                                                                                  >  1:135951/1‑140 (MQ=255)

  gagaAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCCTCGCACATGGCGGAATGTta                                                                                                                                  >  1:139279/1‑140 (MQ=255)

   agaaagaaGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTtat                                                                                                                                 >  1:2054884/1‑140 (MQ=255)

   agaaagaaGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGGGCTTCGCACATGGCGGAATGTtat                                                                                                                                 >  1:1214925/1‑140 (MQ=255)

   agaaagaaGGAATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGAATTGTTCACATTCTGGCTCGTCATGTGGAAGTGGCATTGCACATGGCGGCATGTtat                                                                                                                                 >  2:318283/1‑140 (MQ=255)

            gTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTTTTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACTTTGTGGAATGTTATACCCGCGCa                                                                                                                        >  2:926659/1‑140 (MQ=255)

            gTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCa                                                                                                                        >  1:663571/1‑140 (MQ=255)

             tATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCaa                                                                                                                       <  2:1074340/140‑1 (MQ=255)

             tATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCaa                                                                                                                       <  1:112328/140‑1 (MQ=255)

                  cTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCa                                                                                                                  <  2:1252936/140‑1 (MQ=255)

                  cTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCa                                                                                                                  <  2:530983/140‑1 (MQ=255)

                   tACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAg                                                                                                                 >  1:641649/1‑140 (MQ=255)

                   tACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAg                                                                                                                 >  2:1474152/1‑140 (MQ=255)

                      cGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGa                                                                                                              >  1:365402/1‑140 (MQ=255)

                      cGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGa                                                                                                              >  2:621617/1‑140 (MQ=255)

                       gCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGaa                                                                                                             >  1:1199350/1‑140 (MQ=255)

                        ccTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTTTTCGTCACATTCCGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAAAGGCAGGGAat                                                                                                            >  2:884408/1‑140 (MQ=255)

                        ccTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAat                                                                                                            >  1:1475194/1‑140 (MQ=255)

                        ccTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGCCACAATCTTGCGCGTCATGTGGAAGGGGCTTCGCACATGGCGGAATGTTATACCCGCGCCACGGCAGGGAat                                                                                                            >  2:1864880/1‑140 (MQ=255)

                         cTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCACACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAata                                                                                                           <  1:1508726/140‑1 (MQ=255)

                          ttCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAatat                                                                                                          <  1:1534630/140‑1 (MQ=255)

                          ttCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAatat                                                                                                          <  1:402537/140‑1 (MQ=255)

                           tCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATc                                                                                                         >  1:252786/1‑140 (MQ=255)

                           tCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATc                                                                                                         >  2:483868/1‑140 (MQ=255)

                             ggTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATTGCGGAATGTTTTACCCGCGCAACGGCAGGGAATATCgg                                                                                                       >  1:1635491/1‑140 (MQ=255)

                             ggTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCgg                                                                                                       >  2:841896/1‑140 (MQ=255)

                               tgtTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCg                                                                                                     <  1:115333/140‑1 (MQ=255)

                               tgtTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCg                                                                                                     <  1:841896/140‑1 (MQ=255)

                               tgtTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCg                                                                                                     <  1:2124712/140‑1 (MQ=255)

                               ggtTCCGGGAGCTGCAATCAATCCGTCCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCg                                                                                                     <  1:1361526/139‑1 (MQ=255)

                                 ttCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAGCGGCAGGGAATATCGGCGTa                                                                                                   <  1:439597/140‑1 (MQ=255)

                                 ttCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTa                                                                                                   <  1:1425065/140‑1 (MQ=255)

                                  tCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTAt                                                                                                  <  2:1816846/140‑1 (MQ=255)

                                    cGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGt                                                                                                >  2:742639/1‑140 (MQ=255)

                                         gCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTggg                                                                                           <  2:1420610/140‑1 (MQ=255)

                                         gCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTggg                                                                                           <  2:641649/140‑1 (MQ=255)

                                           tGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTgggg                                                                                          >  1:242210/1‑139 (MQ=255)

                                           tGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTgggg                                                                                          <  2:242210/139‑1 (MQ=255)

                                           tGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGa                                                                                         >  2:396575/1‑140 (MQ=255)

                                            gCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGGATATCCGCGTATGTCTGGGGat                                                                                        >  2:22088/1‑139 (MQ=255)

                                               atcaatcCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTc                                                                                     <  2:663571/140‑1 (MQ=255)

                                                       gTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTg                                                                             <  2:241448/140‑1 (MQ=255)

                                                          cTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCgg                                                                          >  1:2011529/1‑140 (MQ=255)

                                                            aCTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGc                                                                        <  1:742639/140‑1 (MQ=255)

                                                             cTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCa                                                                       <  2:442043/140‑1 (MQ=255)

                                                               cAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACg                                                                     <  2:135951/140‑1 (MQ=255)

                                                               cAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACg                                                                     <  1:1474152/140‑1 (MQ=255)

                                                                  cGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGAc                                                                  >  1:727620/1‑140 (MQ=255)

                                                                  cGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGAc                                                                  >  2:1163159/1‑140 (MQ=255)

                                                                     tGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATg                                                               >  1:111176/1‑140 (MQ=255)

                                                                       cGTAAGCACGGCGGTATTCGTCACATTTTGGCGCCTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGGAAATAGGCGTAAGGCTGGGGGCTTTCGGTCCTGCGGGCACCGACCTGAt                                                             >  2:91864/1‑140 (MQ=255)

                                                                       cGTAAGCACGGCGGTATTCGTCACATTCTTGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATTCCCGCGCAACGGCAGGGAATTTCCGTGTATGTCTGGGGACTTCCTGTCCTGCGGGCACGGACATGAt                                                             >  2:749769/1‑140 (MQ=255)

                                                                           aGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCAcc                                                         <  1:483868/140‑1 (MQ=255)

                                                                            gCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACcg                                                        <  2:1492416/140‑1 (MQ=255)

                                                                              aCGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACcgcg                                                      <  1:1166595/140‑1 (MQ=255)

                                                                              aCGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACcgcg                                                      <  1:1412436/140‑1 (MQ=255)

                                                                                  cggTATTCGTCACATTCTGGCGCGTCATGTGGAAGGGGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGctct                                                  >  2:1300881/1‑140 (MQ=255)

                                                                                          gTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCt                                          >  1:900738/1‑140 (MQ=255)

                                                                                            cacaTTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTc                                        >  2:1657606/1‑140 (MQ=255)

                                                                                               aTTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATTTCGGCGTATGTCTGGGGACTTCCCGTCCTGCGGGCACGGACATGATAACCGCGCTCTATTCCGCTTctct                                     >  2:1733693/1‑138 (MQ=255)

                                                                                               aTTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTctgc                                     >  2:810902/1‑140 (MQ=255)

                                                                                                 tCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTctgctt                                   >  2:148380/1‑139 (MQ=255)

                                                                                                   tGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGat                                 <  1:1295806/140‑1 (MQ=255)

                                                                                                   tGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGat                                 >  1:1513830/1‑140 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               >  2:1205491/1‑140 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:1040413/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:1684890/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:537692/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:55449/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  2:2105553/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  2:2054884/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  2:1221383/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  2:1323628/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:926659/140‑1 (MQ=255)

                                                                                                     gcgcGTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGatcc                               <  1:396575/140‑3 (MQ=255)

                                                                                                       gcgTCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattcca                             <  1:2127520/140‑1 (MQ=255)

                                                                                                          tCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattccattc                          <  2:1199350/140‑1 (MQ=255)

                                                                                                          tCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               >  1:1991990/1‑135 (MQ=255)

                                                                                                          tCATGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  2:1991990/135‑1 (MQ=255)

                                                                                                           aaTGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattccattcc                         <  2:111176/139‑1 (MQ=255)

                                                                                                            aTGTGGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCt                        <  2:766494/140‑1 (MQ=255)

                                                                                                             tgtgGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCtt                       >  2:1842062/1‑139 (MQ=255)

                                                                                                               tgGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTAtt                     <  2:308221/140‑1 (MQ=255)

                                                                                                               tgGAAGGTGCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTAtt                     <  2:2011529/140‑1 (MQ=255)

                                                                                                               aggAAGGTGCCTCGGAAAAGGCGGAATATTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTAtt                     <  2:1089840/139‑1 (MQ=255)

                                                                                                                       gCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               >  2:1051136/1‑122 (MQ=255)

                                                                                                                       gCTTCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGattc                               <  1:1051136/122‑1 (MQ=255)

                                                                                                                         ttCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTa           >  1:175684/1‑140 (MQ=255)

                                                                                                                         ttCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTa           >  1:480756/1‑140 (MQ=255)

                                                                                                                         ttCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTa           >  1:518097/1‑140 (MQ=255)

                                                                                                                         ttCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTa           >  1:1488614/1‑140 (MQ=255)

                                                                                                                         ttCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTa           >  1:701002/1‑140 (MQ=255)

                                                                                                                          tCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTAc          <  1:91864/140‑1 (MQ=255)

                                                                                                                          tCGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTAc          <  1:1657606/140‑1 (MQ=255)

                                                                                                                           cGCACATGGCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTAcc         >  1:100241/1‑140 (MQ=255)

                                                                                                                                  ggCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAgg  <  1:621617/140‑1 (MQ=255)

                                                                                                                                  ggCGGAATGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAgg  >  1:777659/1‑140 (MQ=255)

TGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGG  >  NC_000913/533956‑534225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A