Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	537,063	G→T	G160C (GGC→TGC)	glxR →	tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	537,063	0	G	T	97.6%	266.2 / 0.4	83	G160C (GGC→TGC)	glxR	tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (44/37); total (46/37)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

ATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGAT  >  NC_000913/536926‑537199

aTCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGc                                                                                                                                        >  1:999913/1‑140 (MQ=255)

aTCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGc                                                                                                                                        >  2:775858/1‑140 (MQ=255)

     gATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTaa                                                                                                                                   >  1:591702/1‑140 (MQ=255)

       tGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACg                                                                                                                                 >  1:1558562/1‑140 (MQ=255)

        gcgcCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCCCCCTCGTGTGCGGTAACgg                                                                                                                                <  2:368791/140‑1 (MQ=255)

          gcCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCg                                                                                                                              <  1:455750/140‑1 (MQ=255)

          gcCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCg                                                                                                                              <  2:584993/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  1:352179/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  2:1885637/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  2:1558562/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  1:775858/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  2:1090695/140‑1 (MQ=255)

               tctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  2:999913/140‑1 (MQ=255)

               gctcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGt                                                                                                                         <  2:1029453/139‑1 (MQ=255)

                ctcCGGCGGTGAAATCGGTGCGCGTGAA‑GGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCa                                                                                                                       <  2:793230/140‑1 (MQ=255)

                 tcCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCa                                                                                                                       >  2:1418128/1‑140 (MQ=255)

                    ggcggTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAAc                                                                                                                    >  1:815231/1‑140 (MQ=255)

                    ggcggTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAAc                                                                                                                    >  2:917922/1‑140 (MQ=255)

                        gTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGc                                                                                                                >  2:1551327/1‑140 (MQ=255)

                         tGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCg                                                                                                                              >  1:1418570/1‑125 (MQ=255)

                         tGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCg                                                                                                                              <  2:1418570/125‑1 (MQ=255)

                         tGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCa                                                                                                               <  2:1993963/140‑1 (MQ=255)

                           aaaTCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCTGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGCAAAAATATCACCCTCGTGTGCGGTAACGGCGAGGGTCGAAGCtttcaa                                                                                                             >  2:1601277/1‑135 (MQ=255)

                           aaaTCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCaaa                                                                                                             >  1:330355/1‑140 (MQ=255)

                              tCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTg                                                                                                          <  2:330355/140‑1 (MQ=255)

                                 gTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCa                                                                                                       <  1:781173/140‑1 (MQ=255)

                                   gcgcGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCaaa                                                                                                     <  2:1877589/140‑1 (MQ=255)

                                      cgTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCa                                                                                                  <  1:1324001/140‑1 (MQ=255)

                                      cgTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCa                                                                                                  <  1:1418128/140‑1 (MQ=255)

                                              gACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCg                                                                                          <  2:257915/140‑1 (MQ=255)

                                                cGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAGCCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTg                                                                                        <  1:1727854/140‑1 (MQ=255)

                                                agTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTg                                                                                        <  2:349702/139‑1 (MQ=255)

                                                agTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAAAATCAACCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTg                                                                                        <  2:79723/139‑1 (MQ=255)

                                                   tGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGgcg                                                                                     <  2:315345/140‑1 (MQ=255)

                                                     tCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCt                                                                                   >  1:1259917/1‑140 (MQ=255)

                                                     tCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCt                                                                                   <  2:1383888/140‑1 (MQ=255)

                                                     tCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCt                                                                                   <  2:1259917/140‑1 (MQ=255)

                                                     tCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCACGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCt                                                                                   <  2:1103903/140‑1 (MQ=255)

                                                       gATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCa                                                                                 >  1:998091/1‑140 (MQ=255)

                                                         ttATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAat                                                                               >  1:1051922/1‑140 (MQ=255)

                                                             ggTTGGCGGTGATGAAGTGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGgcg                                                                                     >  2:308009/1‑130 (MQ=255)

                                                             ggTTGGCGGTGATGAAGTGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGgcg                                                                                     <  1:308009/130‑1 (MQ=255)

                                                             ggTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTg                                                                           >  1:873300/1‑140 (MQ=255)

                                                               ttGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGaa                                                                         >  1:192772/1‑140 (MQ=255)

                                                                 ggCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGc                                                                       <  2:1207900/140‑1 (MQ=255)

                                                                 ggCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGc                                                                       >  1:1053538/1‑140 (MQ=255)

                                                                 ggCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGc                                                                       >  1:1477579/1‑140 (MQ=255)

                                                                  gCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCg                                                                      <  2:133875/140‑1 (MQ=255)

                                                                  gCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCg                                                                      <  2:1474686/140‑1 (MQ=255)

                                                                   cGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACACTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCgg                                                                     >  2:1567841/1‑140 (MQ=255)

                                                                          gaAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGa                                                              <  2:998091/140‑1 (MQ=255)

                                                                            aGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAg                                                            >  1:55495/1‑140 (MQ=255)

                                                                            aGCGGTATTTGAACGTGTTAAAACGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAg                                                            <  1:1137093/140‑1 (MQ=255)

                                                                             gCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGc                                                           <  1:1551327/140‑1 (MQ=255)

                                                                             gCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGGAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGGTTCTGAAGc                                                           >  1:1866237/1‑140 (MQ=255)

                                                                              cGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGcc                                                          <  2:2136938/140‑1 (MQ=255)

                                                                                  aTTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGc                                                      >  2:813000/1‑140 (MQ=255)

                                                                                  aTTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGc                                                      >  2:899591/1‑140 (MQ=255)

                                                                                          gtgtTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCt                                              >  2:143341/1‑140 (MQ=255)

                                                                                          gtgtTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCt                                              <  1:1423020/140‑1 (MQ=255)

                                                                                           tgtTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCtt                                             >  1:576247/1‑140 (MQ=255)

                                                                                           tgtTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCtt                                             >  2:1915769/1‑140 (MQ=255)

                                                                                                     cTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCgg                                   >  1:2145514/1‑140 (MQ=255)

                                                                                                     cTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGACAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCgg                                   >  2:1559243/1‑140 (MQ=255)

                                                                                                      tGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGt                                  >  2:995428/1‑140 (MQ=255)

                                                                                                      tGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGt                                  >  2:657889/1‑140 (MQ=255)

                                                                                                             aCTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGAcc                           <  1:657889/140‑1 (MQ=255)

                                                                                                              cTGCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGAccc                          <  1:1915769/140‑1 (MQ=255)

                                                                                                                gCTCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCgg                        >  1:728566/1‑140 (MQ=255)

                                                                                                                  tCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCg                               <  2:939372/131‑1 (MQ=255)

                                                                                                                  tCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCg                               >  1:939372/1‑131 (MQ=255)

                                                                                                                  tCGGTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTa                      >  1:279436/1‑140 (MQ=255)

                                                                                                                    ggTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACg                    >  2:681193/1‑140 (MQ=255)

                                                                                                                     gTAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACgt                   <  1:995428/140‑1 (MQ=255)

                                                                                                                      tAAAAATATCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACgtg                  >  1:386738/1‑140 (MQ=255)

                                                                                                                           atatCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTgcgc             >  1:2087843/1‑140 (MQ=255)

                                                                                                                              tCACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAg          >  2:1003667/1‑140 (MQ=255)

                                                                                                                               cACCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAgg         >  2:275156/1‑140 (MQ=255)

                                                                                                                                aCCCTCGTGTGGGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTTGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGgc        >  1:1806168/1‑140 (MQ=255)

                                                                                                                                aCCCTCGTGTGCGGTAACGGCGATGGTGAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCTATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGgc        >  2:492945/1‑140 (MQ=255)

                                                                                                                                aCCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGgc        >  1:522288/1‑140 (MQ=255)

                                                                                                                                aCCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGgc        >  2:999726/1‑140 (MQ=255)

                                                                                                                                aCCCTCGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGATCAATATTGAAGCGGCTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGgc        >  2:816432/1‑140 (MQ=255)

                                                                                                                                     cGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTgt   >  2:1723346/1‑139 (MQ=255)

                                                                                                                                     cGTGTGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGa   >  1:2008830/1‑140 (MQ=255)

                                                                                                                                      gtgtGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACgtgt                 >  1:276217/1‑125 (MQ=255)

                                                                                                                                      gtgtGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGAt  >  2:259261/1‑140 (MQ=255)

                                                                                                                                      gtgtGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGAt  >  1:358991/1‑140 (MQ=255)

                                                                                                                                      gtgtGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCACTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACgtgt                 <  2:276217/125‑1 (MQ=255)

                                                                                                                                      gTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGAt  >  2:1533774/1‑140 (MQ=255)

ATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGAT  >  NC_000913/536926‑537199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A