Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F48 I1 R1	61	74.3	2613450	99.1%	2589928	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,658	C→A	P327T (CCT→ACT)	fdrA →	putative acyl‑CoA synthetase FdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,658	0	C	A	100.0%	116.5 / NA	34	P327T (CCT→ACT)	fdrA	putative acyl‑CoA synthetase FdrA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/22); total (12/22)

GACGGTGGCGCTGTTTTTAGGTTATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGAT  >  NC_000913/547522‑547792

gACGGTGGCGCTGTTTTTAGGTTATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTg                                                                                                                                       >  2:103886/1‑140 (MQ=255)

       gcgcTGTTTTTAGGTTATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTcagcag                                                                                                                                >  2:974747/1‑140 (MQ=255)

                    gTTATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGc                                                                                                                   <  2:490000/140‑1 (MQ=255)

                     ttATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCg                                                                                                                  <  1:255412/140‑1 (MQ=255)

                       ataCCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGt                                                                                                                <  2:1139489/140‑1 (MQ=255)

                       ataCCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGt                                                                                                                >  1:710530/1‑140 (MQ=255)

                         aCCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGttt                                                                                                              >  1:619643/1‑140 (MQ=255)

                               gcggTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATAc                                                                                                        >  2:282811/1‑140 (MQ=255)

                                   tGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATAc                                                                                                        <  2:674069/136‑1 (MQ=255)

                                   tGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATAc                                                                                                        >  1:674069/1‑136 (MQ=255)

                                   tGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACcggg                                                                                                    >  2:1235129/1‑139 (MQ=255)

                                         gcgACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACg                                                                                              <  2:619643/140‑1 (MQ=255)

                                                       tGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAgc                                                                                >  2:3941/1‑140 (MQ=255)

                                                         gTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAgcgg                                                                              <  1:282811/140‑1 (MQ=255)

                                                                   tcGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACt                                                                   <  2:944914/140‑1 (MQ=255)

                                                                       tGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGcc                                                               <  1:96483/140‑1 (MQ=255)

                                                                         gATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCgg                                                             <  2:598323/140‑1 (MQ=255)

                                                                              ggCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑cc                                                       <  2:83008/140‑1 (MQ=255)

                                                                                ccGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCtt                                                     <  1:602635/140‑1 (MQ=255)

                                                                                  gCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTgg                                                   <  1:1093912/140‑1 (MQ=255)

                                                                                     cGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGt                                                <  1:1235129/140‑1 (MQ=255)

                                                                                              tGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCcga                                       <  1:182063/140‑1 (MQ=255)

                                                                                                 cTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCcgacga                                    <  1:1029573/140‑1 (MQ=255)

                                                                                                 cTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCcgacga                                    <  2:179649/140‑1 (MQ=255)

                                                                                                  tGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTTAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGGTTTCTTTCCGGGCACCCTTGGGGGGGAA‑Ccgacggt                                   >  2:1029573/1‑138 (MQ=255)

                                                                                                  tGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGAt                                   <  1:704733/140‑1 (MQ=255)

                                                                                                      ttCACGCGTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATAccc                               <  1:113209/140‑1 (MQ=255)

                                                                                                          cgcgTCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGGGTTCTTTTCGGGCA‑CCTTTTCGGGGGAGGCGGCGATAtcccacc                           >  2:1234824/1‑136 (MQ=255)

                                                                                                              tCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGGGTTACTTCCCGG‑CA‑CCTTGGCGGGGAAGTCGCGGATATCCATCacact                       >  1:1016477/1‑136 (MQ=255)

                                                                                                              tCACGGCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGGTTATTTTCCCGGCA‑CCCTTGGGGGGGAGCCCGCGATAACCCTCaaaaa                       >  1:1295967/1‑137 (MQ=255)

                                                                                                                  ggCGCGACGTATCGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATGCCGGCGGTGAGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CGTTGGCGTGGAAGCCGACGATACCCATCAACATGGCa                   <  2:762705/140‑1 (MQ=255)

                                                                                                                  ggCGCGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCa                   <  1:472942/140‑1 (MQ=255)

                                                                                                                   gcgcGACGTAACGCAATAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                  <  2:168949/140‑1 (MQ=255)

                                                                                                                       gACGTAACGCAATAGTGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCatgatg              <  2:840468/140‑1 (MQ=255)

                                                                                                                                   tAGCGACTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGAt  <  1:974747/140‑1 (MQ=255)

GACGGTGGCGCTGTTTTTAGGTTATACCCCGGCGGTGGCCCGCGACGAGAATGTCTGGTTTGCCTCCTCGCTGGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGC‑GGGATTACTTGCCGGACA‑CCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGAT  >  NC_000913/547522‑547792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A