Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,783	G→T	D143Y (GAT→TAT)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,783	0	G	T	97.4%	500.4 / 6.9	154	D143Y (GAT→TAT)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (75/75); total (77/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 162 total aligned reads displayed.

CCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCC  >  NC_000913/548646‑548915

ccGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAatat                                                                                                                                    >  2:1278884/1‑140 (MQ=255)

ccGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAatat                                                                                                                                    >  2:954035/1‑140 (MQ=255)

  gTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGt                                                                                                                                  <  1:1852421/140‑1 (MQ=255)

   tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTc                                                                                                                                 >  2:765004/1‑140 (MQ=255)

   tCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTc                                                                                                                                 >  1:52803/1‑140 (MQ=255)

    cGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTcc                                                                                                                                >  1:1278627/1‑140 (MQ=255)

     gggCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCt                                                                                                                               >  1:1766457/1‑140 (MQ=255)

          aaTGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTc                                                                                                                          >  1:1621949/1‑140 (MQ=255)

           aTGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACAGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCg                                                                                                                         <  1:1849843/140‑1 (MQ=255)

                        attCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATg                                                                                                            >  1:1750239/2‑140 (MQ=255)

                          tCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATgcg                                                                                                          <  2:1621949/140‑1 (MQ=255)

                          tCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATgcg                                                                                                          <  2:118669/140‑1 (MQ=255)

                            tGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGcgcg                                                                                                        <  2:377356/140‑1 (MQ=255)

                             gCCAGTATGCCTATGCTGGAGGGTGATAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       >  1:681153/1‑140 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGcgcgt                                                                                                       >  2:735575/1‑139 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGcgcgt                                                                                                       >  2:1960234/1‑139 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       >  1:1652349/1‑140 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       >  1:921468/1‑140 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       >  1:1769224/1‑140 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       >  1:934770/1‑140 (MQ=255)

                             gCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGa                                                                                                       <  1:824697/140‑1 (MQ=255)

                              ccAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGAt                                                                                                      <  1:50897/140‑1 (MQ=255)

                              ccAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGAt                                                                                                      <  2:1769224/140‑1 (MQ=255)

                              ccAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGAt                                                                                                      <  1:1147360/140‑1 (MQ=255)

                              ccAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGAt                                                                                                      >  1:1019823/1‑140 (MQ=255)

                                   aTGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGtt                                                                                                 <  2:381606/140‑1 (MQ=255)

                                   aTGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGtt                                                                                                 >  2:762366/1‑140 (MQ=255)

                                   aTGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGtt                                                                                                 <  1:1023452/140‑1 (MQ=255)

                                   aTGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGtt                                                                                                 <  1:1793760/140‑1 (MQ=255)

                                     gCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                                                                                               >  1:1052854/1‑140 (MQ=255)

                                     gCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                                                                                               <  1:943523/140‑1 (MQ=255)

                                        gATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGc                                                                                            <  2:932706/140‑1 (MQ=255)

                                         aTGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGc                                                                                            <  2:2050312/139‑1 (MQ=255)

                                         aTGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGc                                                                                            >  1:2050312/1‑139 (MQ=255)

                                          tGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCa                                                                                          >  1:997858/1‑140 (MQ=255)

                                          tGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCa                                                                                          <  1:735575/140‑1 (MQ=255)

                                            cTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCACCATCGCTGGATGCGCGATGTGTTAATGCCAGt                                                                                        >  2:1337524/1‑140 (MQ=255)

                                               gtggtTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTAtt                                                                                     >  1:857553/1‑140 (MQ=255)

                                               gtggtTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTAtt                                                                                     <  2:1983742/140‑1 (MQ=255)

                                                    tGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcg                                                                                <  2:1764912/140‑1 (MQ=255)

                                                    tGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcg                                                                                <  2:1211165/140‑1 (MQ=255)

                                                     gagaACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcgc                                                                               >  2:884157/1‑140 (MQ=255)

                                                      agaACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGcgcg                                                                              >  2:520362/1‑140 (MQ=255)

                                                      agaACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGcgcg                                                                              >  2:1084001/1‑140 (MQ=255)

                                                          cGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGgcgc                                                                          >  2:903953/1‑140 (MQ=255)

                                                           gTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCt                                                                         <  1:586400/140‑1 (MQ=255)

                                                           gTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCt                                                                         <  2:1887853/140‑1 (MQ=255)

                                                            tGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTg                                                                        >  1:32902/1‑140 (MQ=255)

                                                            tGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTg                                                                        >  2:1805748/1‑140 (MQ=255)

                                                            ggACCGAGTGCAACCTGGCGTACTAGAGCCTCAACAAAGGGATCTGCAAAGTGAGGCTTTTTTTCTCTTTCGGGGAATATTTCGTTACGCTCCATCTCTTGGTGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCtt                                                                        >  2:2022791/2‑139 (MQ=255)

                                                             gACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTgg                                                                       >  2:1823632/1‑140 (MQ=255)

                                                              aCCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTggg                                                                      >  2:454462/1‑140 (MQ=255)

                                                                    ggCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCAt                                                                >  2:1982286/1‑140 (MQ=255)

                                                                       aaCCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGa                                                             >  2:273123/1‑140 (MQ=255)

                                                                        aCCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAg                                                            <  1:1084001/140‑1 (MQ=255)

                                                                          cGGGCGTTCTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAgcg                                                          <  1:1278884/140‑1 (MQ=255)

                                                                          cGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAgcg                                                          <  2:1699959/140‑1 (MQ=255)

                                                                          cGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAgcg                                                          <  2:423923/140‑1 (MQ=255)

                                                                          cGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAgcg                                                          <  1:497734/140‑1 (MQ=255)

                                                                            ggCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGcgcg                                                        <  1:380453/140‑1 (MQ=255)

                                                                            ggCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGcgcg                                                        <  1:1334067/140‑1 (MQ=255)

                                                                             gCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCgg                                                       >  1:1178748/1‑140 (MQ=255)

                                                                             gCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCgg                                                       >  1:1208399/1‑140 (MQ=255)

                                                                                    gCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGAt                                                >  2:1247905/1‑140 (MQ=255)

                                                                                    gCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGAt                                                >  2:321256/1‑140 (MQ=255)

                                                                                      aaCCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGAtct                                              <  1:1440325/140‑1 (MQ=255)

                                                                                       aCCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGAtctc                                             >  2:859772/1‑140 (MQ=255)

                                                                                       aCCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGAtctc                                             >  2:662314/1‑140 (MQ=255)

                                                                                           cAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACgg                                         >  1:634893/1‑140 (MQ=255)

                                                                                             aCGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCg                                       >  2:1604122/1‑140 (MQ=255)

                                                                                             aCGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCg                                       >  2:476350/1‑140 (MQ=255)

                                                                                                    tATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATgg                                >  1:693740/1‑140 (MQ=255)

                                                                                                      tCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGgcg                              <  1:423847/140‑1 (MQ=255)

                                                                                                      tCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGgcg                              >  2:708816/1‑140 (MQ=255)

                                                                                                      tCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGgcg                              >  1:1574435/1‑140 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGGGTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCGTGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:934770/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:547614/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:1330541/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:693740/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  1:548052/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:1178748/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  1:982225/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            <  2:1168266/140‑1 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATACCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCa                            >  2:545113/1‑140 (MQ=255)

                                                                                                        ggCAAAGTGATGCGTTTTGCGCCTTCCGGCGAATATGTCATGACTCGCCATCGCCGGATGCGTGATGTGTTAATGCCAGTATTAAGCGCAGCTCTGGGGCGCATGGAGCGCGGTATCTATCTCACGGCGATGATGCCGCa                            <  2:1652349/140‑1 (MQ=255)

                                                                                                          cAAAGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAgg                          >  1:46478/1‑140 (MQ=255)

                                                                                                            aaGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGc                        <  2:1032305/140‑1 (MQ=255)

                                                                                                             aGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCa                       >  1:1894861/1‑140 (MQ=255)

                                                                                                             aGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCa                       >  1:1927164/1‑140 (MQ=255)

                                                                                                             aGTGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCa                       >  2:1846222/1‑140 (MQ=255)

                                                                                                               tGATGCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATACCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCAtt                     <  1:545113/140‑1 (MQ=255)

                                                                                                                   gCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGa                 <  2:1754856/140‑1 (MQ=255)

                                                                                                                   gCGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGa                 <  2:428940/140‑1 (MQ=255)

                                                                                                                    cGTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGAt                >  1:1551793/1‑140 (MQ=255)

                                                                                                                     gTTTTGGCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATg               <  1:1604122/140‑1 (MQ=255)

                                                                                                                          ggCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGa          <  1:321256/140‑1 (MQ=255)

                                                                                                                          ggCGCTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGa          <  1:443864/140‑1 (MQ=255)

                                                                                                                           gcgcTTACGGCGAATATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGAt         <  1:1482834/140‑1 (MQ=255)

                                                                                                                                 aCGGCGAATAGGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTc   >  2:1126905/1‑140 (MQ=255)

                                                                                                                                  cGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCCGGGCATTACGATGGGCGATGAGTTcc  <  2:1571640/140‑1 (MQ=255)

CCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCC  >  NC_000913/548646‑548915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A