Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 602,118 C→A P54T (CCG→ACG)  pheP → phenylalanine:H(+) symporter PheP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913602,1180CA100.0% 436.8 / NA 128P54T (CCG→ACG) phePphenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (65/63);  total (65/63)

Only 100 of 129 total aligned reads displayed.

AGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTG  >  NC_000913/601985‑602253
                                                                                                                                     |                                                                                                                                       
aGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTg                                                                                                                                   <  2:793844/140‑1 (MQ=255)
aGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTg                                                                                                                                   <  1:935078/140‑1 (MQ=255)
 gATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGt                                                                                                                                  <  1:634607/140‑1 (MQ=255)
     cTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTg                                                                                                                              <  1:437367/140‑1 (MQ=255)
     cTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTg                                                                                                                              <  2:1663380/140‑1 (MQ=255)
      tGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGc                                                                                                                             >  1:422051/1‑140 (MQ=255)
      tGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGc                                                                                                                             <  1:298843/140‑1 (MQ=255)
      tGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGc                                                                                                                             >  2:1071094/1‑140 (MQ=255)
      tGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGc                                                                                                                             >  2:1493601/1‑140 (MQ=255)
        cGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTg                                                                                                                           >  2:1073468/1‑140 (MQ=255)
        cGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTg                                                                                                                           >  1:475249/1‑140 (MQ=255)
         gTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTgg                                                                                                                          >  1:1685380/1‑140 (MQ=255)
           cGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGc                                                                                                                        >  2:616993/1‑140 (MQ=255)
           cGAATCAAGAGCCGACGCTTCACCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGc                                                                                                                        <  1:1801282/140‑1 (MQ=255)
            gAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCt                                                                                                                       >  1:1131297/1‑140 (MQ=255)
             aaTCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTa                                                                                                                      >  2:527820/1‑140 (MQ=255)
                cAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACgg                                                                                                                   <  1:413133/140‑1 (MQ=255)
                  agagCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCg                                                                                                                 <  2:639918/140‑1 (MQ=255)
                  agagCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCg                                                                                                                 <  1:1071094/140‑1 (MQ=255)
                       cGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGcc                                                                                                            <  2:1060967/140‑1 (MQ=255)
                            cTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTTCTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGat                                                                                                       <  2:1836067/140‑1 (MQ=255)
                                aTCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGatcatc                                                                                                   >  2:1198294/1‑140 (MQ=255)
                                 tCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCg                                                                                                  <  2:176390/140‑1 (MQ=255)
                                  cgcgGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGc                                                                                                 <  2:422051/140‑1 (MQ=255)
                                    cgGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCtt                                                                                               >  1:1117237/1‑140 (MQ=255)
                                    cgGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCtt                                                                                               <  2:2128871/140‑1 (MQ=255)
                                      gATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTc                                                                                             <  1:1078920/140‑1 (MQ=255)
                                       aTTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTTCTGGGCTACGGCGTCGCCGGGATCATCGCTTTcc                                                                                            >  2:1487379/1‑140 (MQ=255)
                                        ttACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCt                                                                                           <  1:1073468/140‑1 (MQ=255)
                                        ttACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCt                                                                                           <  1:860891/140‑1 (MQ=255)
                                        ttACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCt                                                                                           <  1:527820/140‑1 (MQ=255)
                                         tACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTg                                                                                          >  2:1377496/1‑140 (MQ=255)
                                                 cGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATgcg                                                                                  >  1:331224/1‑140 (MQ=255)
                                                 cGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATGGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATgcg                                                                                  >  1:1802819/1‑140 (MQ=255)
                                                   tCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTTCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGcc                                                                                >  1:1578313/1‑140 (MQ=255)
                                                   tCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGcc                                                                                >  1:2068530/1‑140 (MQ=255)
                                                   tCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGcc                                                                                >  1:923400/1‑140 (MQ=255)
                                                   tCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGcc                                                                                >  1:1789012/1‑140 (MQ=255)
                                                     atatTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAg                                                                              >  1:1400401/1‑140 (MQ=255)
                                                     atatTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAg                                                                              >  1:1938695/1‑140 (MQ=255)
                                                          cAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTgg                                                                         <  2:1145272/140‑1 (MQ=255)
                                                           aaCTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTTTTGCTAGGCTACGGCGTCGGCGGGATCATCGCTTTCCCGATTATGCGCCAGCTTGGc                                                                        >  2:223269/1‑140 (MQ=255)
                                                           aaCTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGc                                                                        <  1:1493601/140‑1 (MQ=255)
                                                           aaCTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGc                                                                        >  2:1117320/1‑140 (MQ=255)
                                                             cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                                      >  2:1291403/1‑140 (MQ=255)
                                                             cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                                      >  2:2134149/1‑140 (MQ=255)
                                                             cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                                      <  2:1685380/140‑1 (MQ=255)
                                                                aTTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTTCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAt                                                                   >  2:543334/1‑140 (MQ=255)
                                                                aTTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAt                                                                   >  1:1564521/1‑140 (MQ=255)
                                                                aTTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAt                                                                   >  2:1199766/1‑140 (MQ=255)
                                                                 ttgcgttgcgTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtg                                                                  >  1:471115/1‑140 (MQ=255)
                                                                 ttGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtt                                                                  >  2:233250/1‑139 (MQ=255)
                                                                 ttGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtg                                                                  >  1:801871/1‑140 (MQ=255)
                                                                  tGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtgg                                                                 <  2:331224/140‑1 (MQ=255)
                                                                     gTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtggtgg                                                              <  2:1789012/140‑1 (MQ=255)
                                                                      ttGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATggtggt                                                             >  2:1446966/1‑140 (MQ=255)
                                                                          gTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTgag                                                         <  1:1199766/140‑1 (MQ=255)
                                                                              cgcAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGc                                                     >  1:1431813/1‑140 (MQ=255)
                                                                              cgcAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGc                                                     >  2:1440983/1‑140 (MQ=255)
                                                                              cgcAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGc                                                     >  1:1193935/1‑140 (MQ=255)
                                                                              cgcAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGc                                                     >  2:1168210/1‑140 (MQ=255)
                                                                              cgcAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGT‑GTTGAGGAGcc                                                    >  2:1400480/1‑140 (MQ=255)
                                                                                   ttGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTa                                                >  2:232658/1‑140 (MQ=255)
                                                                                       tACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCg                                            >  1:1981236/1‑140 (MQ=255)
                                                                                            gTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCa                                       >  2:1369756/1‑140 (MQ=255)
                                                                                            gTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCa                                       >  1:897080/1‑140 (MQ=255)
                                                                                             tCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCAt                                      >  1:85861/1‑140 (MQ=255)
                                                                                               tGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCAttt                                    >  2:494604/1‑140 (MQ=255)
                                                                                                 tttCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGc                                  >  2:358128/1‑140 (MQ=255)
                                                                                                    cTTGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCa                               <  2:1431813/140‑1 (MQ=255)
                                                                                                      tGGCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACt                             >  1:1487267/1‑140 (MQ=255)
                                                                                                       ggCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                            <  2:1981236/140‑1 (MQ=255)
                                                                                                       ggCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                            <  1:696225/140‑1 (MQ=255)
                                                                                                       ggCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                            >  1:498627/1‑140 (MQ=255)
                                                                                                       ggCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                            <  2:726235/140‑1 (MQ=255)
                                                                                                       ggCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                            <  1:1369756/140‑1 (MQ=255)
                                                                                                        gCATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGT‑GTTGAGGAGCCGGTATCCTGTTCATTTGCCCACTTTg                          <  1:1400480/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGATCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:475249/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:2105015/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:801871/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  1:2132442/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  1:1074953/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  1:1515978/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  1:1543457/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  1:1944415/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:1056119/140‑1 (MQ=255)
                                                                                                         cATTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:1131297/140‑1 (MQ=255)
                                                                                                         aaTTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                          <  2:62496/139‑1 (MQ=255)
                                                                                                          aTTGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGc                         <  2:1487267/140‑1 (MQ=255)
                                                                                                           ttGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGcc                        >  2:2103243/1‑140 (MQ=255)
                                                                                                           ttGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGcc                        <  1:1377496/140‑1 (MQ=255)
                                                                                                            tGGCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCt                       <  1:2057981/140‑1 (MQ=255)
                                                                                                              gCCCGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATAGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCtat                     <  1:1115950/140‑1 (MQ=255)
                                                                                                                ccGGCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATaa                   <  1:229339/140‑1 (MQ=255)
                                                                                                                   gCGATTCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATa                >  1:929703/1‑140 (MQ=255)
                                                                                                                        tCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATaaa                  >  2:1382410/1‑133 (MQ=255)
                                                                                                                        tCAGATGGCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTggg           >  1:275995/1‑140 (MQ=255)
                                                                                                                               gCGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGtt    <  1:616993/140‑1 (MQ=255)
                                                                                                                                cGGGTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGttt   >  1:1638012/1‑140 (MQ=255)
                                                                                                                                 gggTACGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTg  <  2:1638012/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTG  >  NC_000913/601985‑602253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A