Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 605,314 G→T S37R (AGC→AGA)  nfsB ← NAD(P)H nitroreductase NfsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913605,3140GT100.0% 287.6 / NA 86S37R (AGC→AGA) nfsBNAD(P)H nitroreductase NfsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/40);  total (46/40)

ACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGGCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGAAAGTAAT  >  NC_000913/605177‑605446
                                                                                                                                         |                                                                                                                                    
accacGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCt                                                                                                                                    >  1:1316289/1‑140 (MQ=255)
  cacGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGt                                                                                                                                  >  2:1289484/1‑140 (MQ=255)
   acgacgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTa                                                                                                                                 <  2:171432/140‑1 (MQ=255)
     gacgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTAtt                                                                                                                               >  2:883521/1‑140 (MQ=255)
       cgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGc                                                                                                                             >  1:757260/1‑140 (MQ=255)
       cgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGc                                                                                                                             >  2:234840/1‑140 (MQ=255)
       cgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGc                                                                                                                             >  1:930589/1‑140 (MQ=255)
       cgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGc                                                                                                                             >  1:1938231/1‑140 (MQ=255)
       cgTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGCAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGGATTGc                                                                                                                             >  1:2074350/1‑140 (MQ=255)
         tGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAg                                                                                                                           <  1:513864/140‑1 (MQ=255)
         tGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAg                                                                                                                           >  2:1414277/1‑140 (MQ=255)
           cGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTa                                                                                                                         <  2:1223855/140‑1 (MQ=255)
                cATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGtt                                                                                                                    <  1:1414277/140‑1 (MQ=255)
                    aaGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGa                                                                                                                <  1:39951/140‑1 (MQ=255)
                         ttttACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGc                                                                                                           <  2:507826/140‑1 (MQ=255)
                          tttACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCt                                                                                                          <  2:930589/140‑1 (MQ=255)
                              cGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGc                                                                                                      <  1:1289484/140‑1 (MQ=255)
                              aggtCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGc                                                                                                      <  2:1316289/137‑1 (MQ=255)
                                   gTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGAt                                                                                                               <  2:155805/126‑1 (MQ=255)
                                   gTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGAt                                                                                                               >  1:155805/1‑126 (MQ=255)
                                           cGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTa                                                                                         >  1:1792763/1‑140 (MQ=255)
                                             tAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAg                                                                                       >  1:868436/1‑140 (MQ=255)
                                               aTTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGtt                                                                                     >  2:1208825/1‑140 (MQ=255)
                                                 tACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGtttt                                                                                   >  1:419360/1‑140 (MQ=255)
                                                 tACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGtttt                                                                                   >  2:340540/1‑140 (MQ=255)
                                                 tACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGtttt                                                                                   >  1:144282/1‑140 (MQ=255)
                                                    cGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGTTGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGttttttt                                                                                >  2:171693/1‑139 (MQ=255)
                                                    cGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTg                                                                                <  2:278122/140‑1 (MQ=255)
                                                      gCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCt                                                                              >  2:1779819/1‑140 (MQ=255)
                                                      gCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCt                                                                              >  2:1746328/1‑140 (MQ=255)
                                                       cAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTg                                                                             <  2:1811913/140‑1 (MQ=255)
                                                       cAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTCGATCTGCTCGGCCTGTTCCGGGGTAAGTTCTTTGCTg                                                                             >  2:695483/1‑140 (MQ=255)
                                                        aGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTgg                                                                            >  1:361788/1‑140 (MQ=255)
                                                        aGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTgg                                                                            >  1:331914/1‑140 (MQ=255)
                                                        aGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTgg                                                                            >  2:503941/1‑140 (MQ=255)
                                                          cGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCa                                                                          >  2:2120879/1‑140 (MQ=255)
                                                             aTTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCa                                                                       <  2:2097711/140‑1 (MQ=255)
                                                              tttGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCaa                                                                      <  2:419360/140‑1 (MQ=255)
                                                               ttGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCaaa                                                                     <  1:2120879/140‑1 (MQ=255)
                                                                  gCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGc                                                                  <  2:868436/140‑1 (MQ=255)
                                                                    aaCACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCt                                                                <  1:57026/140‑1 (MQ=255)
                                                                    aaCACGCGCTTTACCTACTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCt                                                                <  1:1597488/140‑1 (MQ=255)
                                                                       acGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAg                                                             <  1:649551/140‑1 (MQ=255)
                                                                            cTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGat                                                        >  2:1116189/1‑139 (MQ=255)
                                                                                aCCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGAc                                                    >  1:2074156/1‑140 (MQ=255)
                                                                                     cttcCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCttt                                               >  1:1815063/1‑140 (MQ=255)
                                                                                          gTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGc                                          <  1:1776277/140‑1 (MQ=255)
                                                                                          gTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCACTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGc                                          <  2:1618838/140‑1 (MQ=255)
                                                                                           tGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGct                                         >  2:325460/1‑139 (MQ=255)
                                                                                           tGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCg                                         >  2:1924526/1‑140 (MQ=255)
                                                                                           tGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGGAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCg                                         >  1:208834/1‑140 (MQ=255)
                                                                                           tGCTGACAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCg                                         >  2:666257/1‑140 (MQ=255)
                                                                                            gCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGa                                        <  1:730682/140‑1 (MQ=255)
                                                                                            gCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGa                                        <  2:2074350/140‑1 (MQ=255)
                                                                                            gCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGa                                        <  1:503941/140‑1 (MQ=255)
                                                                                            gCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGa                                        <  1:759690/140‑1 (MQ=255)
                                                                                                gCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGa                                    >  2:1344520/1‑140 (MQ=255)
                                                                                                gCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGa                                    >  2:1075035/1‑140 (MQ=255)
                                                                                                    aaaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGACTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATg                                <  1:234840/139‑1 (MQ=255)
                                                                                                     aaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGa                               <  1:1208825/140‑1 (MQ=255)
                                                                                                     aaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGa                               <  2:2074156/140‑1 (MQ=255)
                                                                                                     aaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGa                               <  1:666257/140‑1 (MQ=255)
                                                                                                     aaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGa                               <  2:1660338/140‑1 (MQ=255)
                                                                                                     aaTAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGa                               <  1:1075035/140‑1 (MQ=255)
                                                                                                       tAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGata                             <  2:757260/140‑1 (MQ=255)
                                                                                                       tAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTCGATCTGCTCGGCCTGTTCCGGGGTAAGTTCTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGata                             <  1:695483/140‑1 (MQ=255)
                                                                                                         aaaTGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATc                           <  1:340540/140‑1 (MQ=255)
                                                                                                         aaaTGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATc                           <  2:1430747/140‑1 (MQ=255)
                                                                                                         aaaTGCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATc                           <  2:331914/140‑1 (MQ=255)
                                                                                                          aaTGCCGCGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATcc                          >  1:711608/1‑140 (MQ=255)
                                                                                                             gCCACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATa                       <  2:375062/140‑1 (MQ=255)
                                                                                                              ccACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaa                      >  2:627694/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     >  2:924988/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     >  1:1162035/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     >  2:1066304/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     >  1:300959/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     <  1:656763/140‑1 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATaaa                     >  1:460395/1‑140 (MQ=255)
                                                                                                               cACGGCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCATGTTCCGGGGTAAGTTATTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTGTAAGGCGACAGAAATGATATCCATaaa                     <  2:1353171/140‑1 (MQ=255)
                                                                                                                   gCTGGGAGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACt                 <  2:300959/140‑1 (MQ=255)
                                                                                                                         aGTTGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCAtgt           <  2:1792763/140‑1 (MQ=255)
                                                                                                                            tGGTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGaa        >  2:22079/1‑140 (MQ=255)
                                                                                                                             ggTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCTGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGaaa       >  1:1734352/1‑140 (MQ=255)
                                                                                                                              gTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGaaat      >  2:892756/1‑139 (MQ=255)
                                                                                                                              gTGCTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGAAAg      >  1:673296/1‑140 (MQ=255)
                                                                                                                                 cTGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGAAAGTaa   >  2:1712703/1‑140 (MQ=255)
                                                                                                                                  tGGATGGTCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGAAAGTAAt  >  2:663312/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                    
ACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGATGGGCTGTATTGCAGTAGCGTTTTGATCTGCTCGGCCTGTTCCGGGGTAAGTTTTTTGCTGGCATCAAATGCCTTAGTGGAATGACGCTTTAAGGCGACAGAAATGATATCCATAAAGACTCCATGTGAAAGTAAT  >  NC_000913/605177‑605446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A