Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	660,075	C→A	D48Y (GAC→TAC)	lipA ←	lipoyl synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	660,075	0	C	A	100.0%	451.2 / NA	130	D48Y (GAC→TAC)	lipA	lipoyl synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (81/49); total (81/49)

Only 100 of 131 total aligned reads displayed.

CGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTA  >  NC_000913/659943‑660211

cgcCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGa                                                                                                                                   >  1:663773/1‑140 (MQ=255)

 gcCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGaa                                                                                                                                  >  2:509029/1‑140 (MQ=255)

 gcCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGaa                                                                                                                                  >  1:1781778/1‑140 (MQ=255)

      ggATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTg                                                                                                                             >  1:254567/1‑140 (MQ=255)

                 gTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCa                                                                                                                  >  1:1258665/1‑140 (MQ=255)

                 gTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCa                                                                                                                  >  1:1944588/1‑140 (MQ=255)

                  ttGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCAt                                                                                                                 >  1:806319/1‑140 (MQ=255)

                   tGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCAtt                                                                                                                >  1:1855757/1‑140 (MQ=255)

                   tGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCAtt                                                                                                                <  2:1258665/140‑1 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  2:1369470/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  1:345528/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  1:1994569/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  2:1025020/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  2:1397982/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  1:1392653/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  2:1910342/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  2:402060/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTc                                                                                                               >  1:1017411/1‑140 (MQ=255)

                    gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGATTGATTTTCATCCATTc                                                                                                               >  2:888617/1‑140 (MQ=255)

                      tgtgCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCg                                                                                                             <  2:1781778/140‑1 (MQ=255)

                        tgCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGc                                                                                                           >  1:1350852/1‑140 (MQ=255)

                          ccGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCtt                                                                                                         >  2:1964648/1‑140 (MQ=255)

                           cGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTg                                                                                                        <  2:806319/140‑1 (MQ=255)

                            gTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTgc                                                                                                       <  1:900597/140‑1 (MQ=255)

                             tGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTgcg                                                                                                      >  2:1333109/1‑140 (MQ=255)

                               gTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCgca                                                                                                    >  1:420776/1‑140 (MQ=255)

                                    aGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAgg                                                                                               >  2:1855288/1‑140 (MQ=255)

                                         tCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTc                                                                                          >  2:928218/1‑140 (MQ=255)

                                          ccGCCAGGTTAGGGCAGGAGGTTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGAACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTcg                                                                                         >  1:2120095/1‑140 (MQ=255)

                                            gCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTcgcg                                                                                       <  2:663773/140‑1 (MQ=255)

                                                  ttAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGTCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGt                                                                                 >  2:1543258/1‑140 (MQ=255)

                                                      ggCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGcc                                                                             <  1:340911/140‑1 (MQ=255)

                                                      ggCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGcc                                                                             <  1:720144/140‑1 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  1:1501497/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  1:1273187/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  2:385953/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  2:238394/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  2:1777535/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGtt                                                                        >  1:2013952/1‑140 (MQ=255)

                                                           gaggCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGACTTCGCGCTCTGTTGCCACGtt                                                                        >  1:1793720/1‑140 (MQ=255)

                                                                ttCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTaa                                                                   <  2:1392653/140‑1 (MQ=255)

                                                                 tCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCGTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAAc                                                                  <  1:723665/140‑1 (MQ=255)

                                                                 tCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAAc                                                                  <  1:2012336/140‑1 (MQ=255)

                                                                 tCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAAc                                                                  >  2:314291/1‑140 (MQ=255)

                                                                     cGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCggg                                                              <  1:928218/140‑1 (MQ=255)

                                                                          acagaATGCAGGCCATTTTTGCGCATTGTGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAg                                                         >  2:357661/1‑140 (MQ=255)

                                                                             gaATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGc                                                      >  2:2033212/1‑140 (MQ=255)

                                                                              aaTGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGcc                                                     <  2:1350852/140‑1 (MQ=255)

                                                                              aaTGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCACAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGcc                                                     <  1:402060/140‑1 (MQ=255)

                                                                                tGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCAt                                                   <  1:1211088/140‑1 (MQ=255)

                                                                                    ggCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCttt                                               >  2:1858130/1‑139 (MQ=255)

                                                                                    ggCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCttt                                               >  2:1145474/1‑139 (MQ=255)

                                                                                     gCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAt                                              >  1:405901/1‑140 (MQ=255)

                                                                                      ccATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACGGGGATAAGGGCCATCTTATc                                             >  1:1349572/1‑140 (MQ=255)

                                                                                      ccATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATc                                             >  1:39698/1‑140 (MQ=255)

                                                                                        aTTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAt                                              >  2:1954912/1‑137 (MQ=255)

                                                                                        aTTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAt                                              <  1:1954912/137‑1 (MQ=255)

                                                                                        aTTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCgg                                           >  2:698277/1‑140 (MQ=255)

                                                                                        aTTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAACgg                                           >  2:46805/1‑140 (MQ=255)

                                                                                        aTTTTTGCGAATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCgg                                           >  2:1381752/1‑140 (MQ=255)

                                                                                          ttttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCa                                         >  1:154656/1‑140 (MQ=255)

                                                                                          ttttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCa                                         >  1:1898580/1‑140 (MQ=255)

                                                                                           tttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCAt                                        <  2:184564/140‑1 (MQ=255)

                                                                                               cgcATTGCGGCTTTGATGCCGTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcgcg                                    <  2:832639/140‑1 (MQ=255)

                                                                                               cgcATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcgcg                                    <  1:84162/140‑1 (MQ=255)

                                                                                                    tGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTAtt                               >  1:2904/1‑140 (MQ=255)

                                                                                                    tGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTAtt                               >  2:898926/1‑140 (MQ=255)

                                                                                                    tGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAACGGCATCGCGGTAtt                               >  2:207249/1‑140 (MQ=255)

                                                                                                      cGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCACAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGTCATCGCGGTATTTa                             >  1:1599693/1‑140 (MQ=255)

                                                                                                              aTGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACcgcg                     >  1:1165094/1‑140 (MQ=255)

                                                                                                              aTGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACcgcg                     >  1:351748/1‑140 (MQ=255)

                                                                                                              aTGCCCTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACcgcg                     <  1:898926/140‑1 (MQ=255)

                                                                                                                   cTGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCa                >  2:1383355/1‑140 (MQ=255)

                                                                                                                    tGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCAt               <  1:314291/140‑1 (MQ=255)

                                                                                                                    tGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCAt               <  2:405901/140‑1 (MQ=255)

                                                                                                                    tGGATACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCAt               <  1:385953/140‑1 (MQ=255)

                                                                                                                       aTACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATcac            <  1:2071423/140‑1 (MQ=255)

                                                                                                                        tACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATcaca           <  2:784121/140‑1 (MQ=255)

                                                                                                                        tACGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATcaca           <  1:1397982/140‑1 (MQ=255)

                                                                                                                         aCGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa          <  1:1910342/140‑1 (MQ=255)

                                                                                                                         aCGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa          <  1:794125/140‑1 (MQ=255)

                                                                                                                         aCGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa          <  1:1777535/140‑1 (MQ=255)

                                                                                                                         aCGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa          <  2:1251020/140‑1 (MQ=255)

                                                                                                                         aCGTGTAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAAGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa          <  2:1273187/140‑1 (MQ=255)

                                                                                                                           gtgtAGATTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATg        >  1:1403908/1‑140 (MQ=255)

                                                                                                                           gtgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAGGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATg        >  2:1626572/1‑140 (MQ=255)

                                                                                                                           gtgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAacac                         >  1:565024/1‑123 (MQ=255)

                                                                                                                           gtgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATg        >  2:489419/1‑140 (MQ=255)

                                                                                                                           gtgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATg        <  1:767110/140‑1 (MQ=255)

                                                                                                                            tgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATgg       >  1:1309242/1‑140 (MQ=255)

                                                                                                                            tgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATgg       >  2:1098977/1‑140 (MQ=255)

                                                                                                                            tgtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATgg       >  2:326442/1‑140 (MQ=255)

                                                                                                                             gtAGAGTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATggg      <  1:1621457/140‑1 (MQ=255)

                                                                                                                               agagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt    <  1:1381752/140‑1 (MQ=255)

                                                                                                                               agagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt    <  1:1224533/140‑1 (MQ=255)

                                                                                                                               agagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt    <  2:2000918/140‑1 (MQ=255)

                                                                                                                               agagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt    <  2:154656/140‑1 (MQ=255)

                                                                                                                                gagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGttt   >  2:342365/1‑140 (MQ=255)

                                                                                                                                gagTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGttt   >  2:1554338/1‑140 (MQ=255)

                                                                                                                                 agTACGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTa  >  2:847826/1‑140 (MQ=255)

CGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTA  >  NC_000913/659943‑660211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A