Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	660,409	C→A	intergenic (‑193/+16)	lipA ← / ← ybeF	lipoyl synthase/putative LysR‑type DNA‑binding transcriptional regulator YbeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	660,409	0	C	A	99.2%	441.3 / ‑3.3	129	intergenic (‑193/+16)	lipA/ybeF	lipoyl synthase/putative LysR‑type DNA‑binding transcriptional regulator YbeF
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (45/83); total (45/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 130 total aligned reads displayed.

TGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGCTGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTT  >  NC_000913/660274‑660545

  tAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTaaa                                                                                                                                    >  2:1141411/1‑139 (MQ=255)

  tAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAAc                                                                                                                                    >  2:629713/1‑140 (MQ=255)

      tATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATa                                                                                                                                <  1:777882/140‑1 (MQ=255)

       aTCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATat                                                                                                                               >  2:1628740/1‑139 (MQ=255)

              aTTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCa                                                                                                                        <  1:580218/140‑1 (MQ=255)

              aTTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCa                                                                                                                        <  2:1963380/140‑1 (MQ=255)

              aTTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCa                                                                                                                        <  2:949678/140‑1 (MQ=255)

               tttttGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCaa                                                                                                                       >  1:1811263/1‑140 (MQ=255)

                ttttGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGATAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCaaa                                                                                                                      <  1:847823/140‑1 (MQ=255)

                  ttGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAAt                                                                                                                    <  1:897887/140‑1 (MQ=255)

                      aTTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCAt                                                                                                                <  2:805443/140‑1 (MQ=255)

                      aTTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCAt                                                                                                                >  1:1365898/1‑140 (MQ=255)

                       ttAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCAtt                                                                                                               <  1:593168/140‑1 (MQ=255)

                       ttAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCAtt                                                                                                               <  2:1898580/140‑1 (MQ=255)

                        tAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTa                                                                                                              <  2:440501/140‑1 (MQ=255)

                        tAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTa                                                                                                              <  1:1628740/140‑1 (MQ=255)

                        tAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTa                                                                                                              <  2:44525/140‑1 (MQ=255)

                        tAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTa                                                                                                              <  1:932287/140‑1 (MQ=255)

                         aaCGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTAc                                                                                                             >  1:246014/1‑140 (MQ=255)

                          aCGACTGGTAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACg                                                                                                            <  1:1982014/140‑1 (MQ=255)

                          aCGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACg                                                                                                            <  2:243934/140‑1 (MQ=255)

                           cGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACgg                                                                                                           <  1:1212953/140‑1 (MQ=255)

                            gACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGa                                                                                                          <  1:103033/140‑1 (MQ=255)

                            gACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGa                                                                                                          <  2:389098/140‑1 (MQ=255)

                            gACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGa                                                                                                          <  1:397557/140‑1 (MQ=255)

                              cTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGCTGTAACAATACTTTTTCAAAATGCATTACGGATa                                                                                                        <  1:1271470/140‑1 (MQ=255)

                              cTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATa                                                                                                        <  1:615508/140‑1 (MQ=255)

                              cTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATa                                                                                                        <  1:1728339/140‑1 (MQ=255)

                              atGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATa                                                                                                        <  2:805077/139‑1 (MQ=255)

                                ggCAGTATGTCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:1098977/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:1899240/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  2:1924864/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:1393774/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:943486/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  2:360169/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  2:1365898/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:896063/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:1315943/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:359407/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:342365/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  1:1834498/140‑1 (MQ=255)

                                ggCAGTATATCATTGAAACGGACCCGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAAc                                                                                                      <  2:1245750/140‑1 (MQ=255)

                                       tatCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTAtt                                                                                               >  2:1845189/1‑140 (MQ=255)

                                          cATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACt                                                                                            <  2:898873/140‑1 (MQ=255)

                                          cATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACt                                                                                            <  1:6874/140‑1 (MQ=255)

                                          cATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACt                                                                                            <  1:1184615/140‑1 (MQ=255)

                                            ttGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTcc                                                                                          >  1:1561034/1‑140 (MQ=255)

                                            ttGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTcc                                                                                          >  1:1702003/1‑140 (MQ=255)

                                             tGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTcct                                                                                         >  2:647046/1‑139 (MQ=255)

                                             tGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCg                                                                                         >  2:1779861/1‑140 (MQ=255)

                                             tGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCg                                                                                         >  1:365409/1‑140 (MQ=255)

                                              gAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGt                                                                                        >  2:614212/1‑140 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  2:11825/140‑1 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       >  1:766525/1‑140 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  2:1669162/140‑1 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       >  1:1336874/1‑140 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  1:1363648/140‑1 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       >  1:673814/1‑140 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  1:647046/140‑1 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  1:1550981/140‑1 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       >  1:545036/1‑140 (MQ=255)

                                               aaaCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTg                                                                                       <  2:537426/140‑1 (MQ=255)

                                                 aCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCa                                                                                     >  2:356935/1‑140 (MQ=255)

                                                 aCGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCa                                                                                     >  1:246613/1‑140 (MQ=255)

                                                   ggACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAgg                                                                                   >  2:53693/1‑140 (MQ=255)

                                                    gACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGa                                                                                  <  1:1380216/140‑1 (MQ=255)

                                                              cagcCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACg                                                                        <  2:1388326/140‑1 (MQ=255)

                                                              cagcCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACg                                                                        >  2:1623165/1‑140 (MQ=255)

                                                               agcCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGTATGGGATCACGc                                                                       >  2:1107705/1‑140 (MQ=255)

                                                               agcCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGc                                                                       >  1:104177/1‑140 (MQ=255)

                                                                 ccAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAg                                                                     >  1:1429981/1‑140 (MQ=255)

                                                                           cgcgAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATttg                                                           <  2:1486582/140‑1 (MQ=255)

                                                                           cgcgAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATttg                                                           >  1:894503/1‑140 (MQ=255)

                                                                           cgcgAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATttg                                                           <  1:442695/140‑1 (MQ=255)

                                                                             cgAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATttgtt                                                         <  1:53693/140‑1 (MQ=255)

                                                                               aaaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTtgttgt                                                       <  2:46593/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  1:1151910/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:1302413/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:65780/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:1918347/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  1:230913/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:542639/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:1987433/140‑1 (MQ=255)

                                                                                aaaTGTAAAATTGCTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGAGCAGGATGGGATCACGCAGGCTGAATTTGTTGTa                                                      <  2:1685797/140‑1 (MQ=255)

                                                                                       aaaTTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATa                                               <  1:439131/140‑1 (MQ=255)

                                                                                          ttgttgttgGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATagag                                            <  1:1845189/140‑1 (MQ=255)

                                                                                             ttgttgGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGaaa                                         >  2:1466600/1‑140 (MQ=255)

                                                                                              tgttgGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAAt                                        <  1:923326/140‑1 (MQ=255)

                                                                                                  ggATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTAt                                    <  1:629713/140‑1 (MQ=255)

                                                                                                  ggATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTAt                                    <  1:1141411/140‑1 (MQ=255)

                                                                                                   gATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTAtt                                   <  2:1735342/140‑1 (MQ=255)

                                                                                                       gtgCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGct                               >  1:1923745/1‑140 (MQ=255)

                                                                                                       gtgCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGct                               <  2:869293/140‑1 (MQ=255)

                                                                                                        tgCCATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGctc                              <  2:894503/140‑1 (MQ=255)

                                                                                                          ccATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGctcct                            >  1:736284/1‑140 (MQ=255)

                                                                                                          ccATTTTATCGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGctcct                            <  1:1779861/140‑1 (MQ=255)

                                                                                                                   cGTTCTGCGCTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAgg                   <  2:1255663/140‑1 (MQ=255)

                                                                                                                          cgcTGTGATCTGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGaaa            >  2:1106975/1‑140 (MQ=255)

                                                                                                                                    tGGATGTGACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGtttt  >  1:631523/1‑140 (MQ=255)

                                                                                                                                    tGGATGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGtttt  >  1:883951/1‑140 (MQ=255)

TGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGACCTGAAAGCAGCCAAAGCGGTCGCGAAAATGTAAAATTGTTGTTGGATTGTGCCATTTTATCGTTCTGCGCTGTGATCTGGCTGTAACAATACTTTTTCAAAATGCATTACGGATAACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTT  >  NC_000913/660274‑660545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A