Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	720,218	G→T	V81V (GTC→GTA)	speF ←	ornithine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	720,218	0	G	T	99.1%	374.0 / ‑3.2	111	V81V (GTC→GTA)	speF	ornithine decarboxylase, degradative
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (57/53); total (58/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

Only 100 of 112 total aligned reads displayed.

TCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAA  >  NC_000913/720081‑720350

tCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATac                                                                                                                                    >  2:2055051/1‑140 (MQ=255)

tCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATac                                                                                                                                    <  1:1163303/140‑1 (MQ=255)

tCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATac                                                                                                                                    >  1:125949/1‑140 (MQ=255)

  aaaCGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATacac                                                                                                                                  >  1:877880/1‑140 (MQ=255)

     cgcgCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCg                                                                                                                               >  2:1750953/1‑140 (MQ=255)

     agcgcTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCg                                                                                                                               <  2:877880/139‑1 (MQ=255)

         cTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAAt                                                                                                                           >  1:653025/1‑140 (MQ=255)

          tGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATa                                                                                                                          >  2:1183870/1‑140 (MQ=255)

            ttACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACg                                                                                                                        >  1:1411154/1‑140 (MQ=255)

                  tgattgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCaa                                                                                                                  <  1:1453478/140‑1 (MQ=255)

                    attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAAt                                                                                                                >  1:615148/1‑140 (MQ=255)

                    attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAAt                                                                                                                >  2:1712646/1‑140 (MQ=255)

                     ttgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATa                                                                                                               <  1:1381604/140‑1 (MQ=255)

                     ttgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATa                                                                                                               <  2:928174/140‑1 (MQ=255)

                      tgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATat                                                                                                              >  1:552603/1‑139 (MQ=255)

                      tgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATAc                                                                                                              >  1:187599/1‑140 (MQ=255)

                        aCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATActc                                                                                                            >  2:440463/1‑140 (MQ=255)

                        aCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATActc                                                                                                            <  1:2055051/140‑1 (MQ=255)

                         aaTAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATActct                                                                                                           <  2:814389/139‑1 (MQ=255)

                              tCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCggg                                                                                                      >  2:476732/1‑140 (MQ=255)

                                gACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTa                                                                                                    <  1:1712646/140‑1 (MQ=255)

                                gACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTa                                                                                                    <  1:1750953/140‑1 (MQ=255)

                                 aCCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTac                                                                                                   >  1:1908683/1‑140 (MQ=255)

                                  ccAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTaca                                                                                                  >  1:1997777/1‑140 (MQ=255)

                                  ccAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTaca                                                                                                  >  1:1739674/1‑140 (MQ=255)

                                  ccAGTACGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTaca                                                                                                  <  2:1314367/140‑1 (MQ=255)

                                   cAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTacac                                                                                                 <  1:476732/140‑1 (MQ=255)

                                   cAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTacac                                                                                                 >  2:1492170/1‑140 (MQ=255)

                                   cAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTacac                                                                                                 >  1:957439/1‑140 (MQ=255)

                                     gTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGt                                                                                               >  1:1622375/1‑140 (MQ=255)

                                      tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTTGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGtt                                                                                              >  2:1177497/1‑140 (MQ=255)

                                      tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGtt                                                                                              >  2:1872462/1‑140 (MQ=255)

                                      tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGtt                                                                                              >  1:1958015/1‑140 (MQ=255)

                                      tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGtt                                                                                              >  1:841117/1‑140 (MQ=255)

                                        cgcgGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCt                                                                                            <  2:1820205/140‑1 (MQ=255)

                                        cgcgGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGGACACGTTCt                                                                                            >  1:13688/1‑140 (MQ=255)

                                             aagaaAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGAtt                                                                                       >  1:2080652/1‑140 (MQ=255)

                                                aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTc                                                                                    >  2:1882013/1‑140 (MQ=255)

                                                aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTc                                                                                    <  1:91705/140‑1 (MQ=255)

                                                  aGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                  <  2:125949/140‑1 (MQ=255)

                                                  aGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                  <  1:1134900/140‑1 (MQ=255)

                                                  aGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                  <  1:1654764/140‑1 (MQ=255)

                                                     tGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAg                                                                               <  2:967683/140‑1 (MQ=255)

                                                      gggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGc                                                                              <  1:248063/140‑1 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGAtttt                                                                                     >  2:1437319/1‑132 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGAtttt                                                                                     <  1:1437319/132‑1 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  2:1478552/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  1:914775/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  1:159987/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  2:1795969/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             <  2:1958015/140‑1 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  2:301039/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  2:1582528/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  1:364721/1‑140 (MQ=255)

                                                       ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                             >  2:2134426/1‑140 (MQ=255)

                                                        gcgcAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaa                                                                            <  2:187599/140‑1 (MQ=255)

                                                         cgcAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaat                                                                           >  1:693121/1‑140 (MQ=255)

                                                          gcAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaata                                                                          >  1:698019/1‑140 (MQ=255)

                                                             gTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaataaat                                                                       <  1:1663227/140‑1 (MQ=255)

                                                                   tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                 <  1:1080205/140‑1 (MQ=255)

                                                                   tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                 <  2:1552947/140‑1 (MQ=255)

                                                                   tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                 <  1:440463/140‑1 (MQ=255)

                                                                   tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                 <  2:1234734/140‑1 (MQ=255)

                                                                   tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                 <  2:738987/140‑1 (MQ=255)

                                                                    ttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGa                                                                <  2:914775/140‑1 (MQ=255)

                                                                      cATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATg                                                              >  2:1689792/1‑140 (MQ=255)

                                                                       aTAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGc                                                             <  1:1882013/140‑1 (MQ=255)

                                                                             gctggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAAc                                                       >  1:1451627/1‑140 (MQ=255)

                                                                                ggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCg                                                    >  2:1340526/1‑140 (MQ=255)

                                                                                ggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCg                                                    >  1:612767/1‑140 (MQ=255)

                                                                                 gcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGt                                                   >  2:62807/1‑140 (MQ=255)

                                                                                 gcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGt                                                   <  1:1177497/140‑1 (MQ=255)

                                                                                  cAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTg                                                  >  1:1181645/1‑140 (MQ=255)

                                                                                   aGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTgg                                                 >  2:1487830/1‑140 (MQ=255)

                                                                                       gTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATc                                             >  2:327678/1‑140 (MQ=255)

                                                                                                tGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATc                                    <  2:1411154/140‑1 (MQ=255)

                                                                                                 ggCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCg                                   >  1:108821/1‑140 (MQ=255)

                                                                                                  gCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGa                                  >  1:186065/1‑140 (MQ=255)

                                                                                                  gCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGa                                  >  2:400140/1‑140 (MQ=255)

                                                                                                    gACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGt                                >  2:436620/1‑140 (MQ=255)

                                                                                                       cATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGtttt                             <  1:1340526/140‑1 (MQ=255)

                                                                                                       cATAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGtttt                             <  1:2134426/140‑1 (MQ=255)

                                                                                                       aaTAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGTGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGACATAACCCGTGGCATCGATTTCATCGAGtttt                             <  2:1622375/139‑1 (MQ=255)

                                                                                                       aaTAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGtttt                             <  2:1711256/139‑1 (MQ=255)

                                                                                                         tAAAATTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTcc                           <  1:1687023/140‑1 (MQ=255)

                                                                                                          aaaaTTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCg                          <  2:1014310/140‑1 (MQ=255)

                                                                                                            aaTTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCa                        <  2:1908683/140‑1 (MQ=255)

                                                                                                            aaTTCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCa                        <  2:1866151/140‑1 (MQ=255)

                                                                                                              ttCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATg                      <  2:1358431/140‑1 (MQ=255)

                                                                                                              ttCTCGTCGCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATg                      <  2:693121/140‑1 (MQ=255)

                                                                                                                 tcgtcgCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTaa                   <  1:1795969/140‑1 (MQ=255)

                                                                                                                   gtcgCGATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACa                 >  2:1066502/1‑140 (MQ=255)

                                                                                                                        gATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGtt            >  1:2090415/1‑140 (MQ=255)

                                                                                                                        gATTCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGtt            <  2:186065/140‑1 (MQ=255)

                                                                                                                          ttCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGa          <  2:615148/140‑1 (MQ=255)

                                                                                                                          ttCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGa          <  2:2080652/140‑1 (MQ=255)

                                                                                                                          ttCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGa          <  1:1183870/140‑1 (MQ=255)

                                                                                                                           tCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAGCCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAg         <  2:957439/140‑1 (MQ=255)

                                                                                                                           tCGCAATTCTCAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAg         <  1:327678/140‑1 (MQ=255)

                                                                                                                                  tctcAAATACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATaaa  <  2:1997777/140‑1 (MQ=255)

TCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAA  >  NC_000913/720081‑720350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A