Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	729,808	C→A	R76S (CGC→AGC)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	729,808	0	C	A	98.8%	216.5 / ‑2.9	84	R76S (CGC→AGC)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (31/52); total (32/52)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCA  >  NC_000913/729671‑729937

cccccACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGc                                                                                                                                 <  2:1440114/140‑1 (MQ=37)

  cccACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCAc                                                                                                                               >  1:1845559/1‑140 (MQ=37)

    cACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCt                                                                                                                             <  1:477910/140‑1 (MQ=255)

     aCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTa                                                                                                                            >  1:260886/1‑140 (MQ=37)

     aCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTa                                                                                                                            <  1:1882672/140‑1 (MQ=255)

     aCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTa                                                                                                                            <  2:113579/140‑1 (MQ=255)

     aCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGGGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTa                                                                                                                            >  1:1028283/1‑140 (MQ=255)

        ggCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGGGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTAcag                                                                                                                         >  1:315639/1‑140 (MQ=255)

              gCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTa                                                                                                                   >  1:473895/1‑140 (MQ=37)

                   ttCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGa                                                                                                              >  2:1200061/1‑140 (MQ=37)

                   ttCGGTGTGCCCCGGCGGGGGGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGa                                                                                                              >  1:669125/1‑140 (MQ=255)

                        tgtgCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACaaaa                                                                                                         >  2:2057710/1‑140 (MQ=38)

                        tgtgCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACaaaa                                                                                                         >  2:52332/1‑140 (MQ=38)

                        tgtgCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACaaaa                                                                                                         >  2:588175/1‑140 (MQ=38)

                            ccccGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGc                                                                                                     >  2:1422500/1‑140 (MQ=37)

                             cccGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGcc                                                                                                    >  2:1180661/1‑140 (MQ=37)

                             cccGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGcc                                                                                                    >  2:690564/1‑140 (MQ=38)

                             cccGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGcc                                                                                                    >  2:936159/1‑140 (MQ=37)

                             cccGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGACCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGcc                                                                                                    >  1:1181443/1‑140 (MQ=255)

                               cggcggGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCcg                                                                                                  <  2:1564553/140‑1 (MQ=37)

                               cggcggGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCcg                                                                                                  <  1:529628/140‑1 (MQ=37)

                               cggcggGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCcg                                                                                                  <  1:366710/140‑1 (MQ=37)

                                 gcggGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCcgcg                                                                                                >  2:950896/1‑140 (MQ=38)

                                 gcggGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCcgcg                                                                                                <  1:1744796/140‑1 (MQ=37)

                                         aCGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTggg                                                                                        <  1:1200061/140‑1 (MQ=21)

                                          cGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGACCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTgggg                                                                                       <  2:1181443/140‑1 (MQ=21)

                                            tCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAg                                                                                     >  1:2101307/1‑140 (MQ=34)

                                            tCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAg                                                                                     >  1:526074/1‑140 (MQ=21)

                                            tCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAg                                                                                     >  1:648210/1‑140 (MQ=21)

                                            tCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAg                                                                                     >  2:668241/1‑140 (MQ=21)

                                               ggCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCt                                                                                  <  1:1180661/140‑1 (MQ=21)

                                                 ccATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCg                                                                                <  1:1748444/140‑1 (MQ=21)

                                                   aTCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGc                                                                              <  1:690564/140‑1 (MQ=21)

                                                   aTCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGACCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGc                                                                              <  1:2057710/140‑1 (MQ=21)

                                                    tCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcc                                                                             >  1:1814460/1‑140 (MQ=21)

                                                    tCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcc                                                                             <  2:876202/140‑1 (MQ=21)

                                                    tCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCGACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcc                                                                             <  2:2147618/140‑1 (MQ=21)

                                                     ccGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGccc                                                                            >  1:1702751/1‑140 (MQ=25)

                                                         tCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGc                                                                        <  1:1288100/140‑1 (MQ=21)

                                                              ccGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGaa                                                                   <  2:1022850/140‑1 (MQ=21)

                                                                  tgctCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGGTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATg                                                               >  1:2064011/1‑140 (MQ=17)

                                                                   gctCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGc                                                              <  2:603730/140‑1 (MQ=17)

                                                                      cGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTg                                                           <  2:1133944/140‑1 (MQ=17)

                                                                      cGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTg                                                           <  2:1602778/140‑1 (MQ=17)

                                                                        gTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCg                                                         <  1:1537065/140‑1 (MQ=17)

                                                                          gCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGa                                                       <  1:52332/140‑1 (MQ=17)

                                                                          gCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGa                                                       <  2:1028283/140‑1 (MQ=17)

                                                                             aaGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                    <  1:1712284/140‑1 (MQ=17)

                                                                             aaGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                    <  2:928421/140‑1 (MQ=17)

                                                                             aaGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                    <  1:1252255/140‑1 (MQ=17)

                                                                              aGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATc                                                   >  2:741966/1‑140 (MQ=17)

                                                                              aGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATc                                                   >  1:1968836/1‑140 (MQ=17)

                                                                                  ccTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCt                                               <  1:1247717/140‑1 (MQ=17)

                                                                                   cTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCtt                                              >  2:2026042/1‑140 (MQ=17)

                                                                                   cTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCtt                                              >  1:1025837/1‑140 (MQ=17)

                                                                                     tCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTAc                                            >  2:1047227/1‑140 (MQ=17)

                                                                                     tCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTAc                                            >  1:1700362/1‑140 (MQ=17)

                                                                                          gTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                       >  1:1444690/1‑140 (MQ=17)

                                                                                          gTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                       >  2:1183394/1‑140 (MQ=17)

                                                                                          gTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                       <  1:588175/140‑1 (MQ=17)

                                                                                          gTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                       <  1:741966/140‑1 (MQ=17)

                                                                                           tGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCttc                                      >  2:1099687/1‑138 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  2:535802/140‑1 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  2:315639/140‑1 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  1:936159/140‑1 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  2:1873594/140‑1 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  2:648210/140‑1 (MQ=17)

                                                                                            gAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                     <  2:1377708/140‑1 (MQ=17)

                                                                                               aCCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGa                                  <  2:1847864/140‑1 (MQ=17)

                                                                                               aCCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGa                                  <  2:1586343/140‑1 (MQ=17)

                                                                                                 cGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATa                                <  1:1183394/140‑1 (MQ=17)

                                                                                                  gACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATaa                               <  2:1444690/140‑1 (MQ=17)

                                                                                                    cATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAaca                             <  1:1047227/140‑1 (MQ=17)

                                                                                                    cATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAaca                             <  1:2026042/140‑1 (MQ=17)

                                                                                                      tCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAacaca                           <  2:1700362/140‑1 (MQ=17)

                                                                                                          ccTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGa                       <  2:1702751/140‑1 (MQ=17)

                                                                                                          ccTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGa                       <  1:739336/140‑1 (MQ=17)

                                                                                                          ccTGCCCGGCCCGCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGa                       <  1:668241/140‑1 (MQ=17)

                                                                                                             gcccggcccgCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATAc                    <  2:473895/140‑1 (MQ=17)

                                                                                                             gcccggcccgCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATAc                    <  2:205861/140‑1 (MQ=17)

                                                                                                               ccggcccgCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTc                  <  1:950896/140‑1 (MQ=17)

                                                                                                                cggcccgCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCa                 <  1:1874442/140‑1 (MQ=17)

                                                                                                                   cccgCTGCCGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTg              >  2:617804/1‑140 (MQ=18)

                                                                                                                          ccGTTCATTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAAcgg       <  2:1968836/140‑1 (MQ=17)

                                                                                                                               aaTTCTCTCCAGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCa  <  2:1845559/139‑1 (MQ=17)

CCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCA  >  NC_000913/729671‑729937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A