Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	764,442	C→A	A88E (GCA→GAA)	sucD →	succinyl‑CoA synthetase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	764,442	0	C	A	100.0%	462.5 / NA	133	A88E (GCA→GAA)	sucD	succinyl‑CoA synthetase subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (74/59); total (74/59)

Only 100 of 137 total aligned reads displayed.

AAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGCAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTCCGGGTGAAT  >  NC_000913/764307‑764576

  ggcggcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGCAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCAt                                                                                                                                  >  2:1205229/1‑140 (MQ=255)

  ggcggcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCAt                                                                                                                                  <  2:3819/140‑1 (MQ=255)

    cggcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCa                                                                                                                                <  1:440229/140‑1 (MQ=255)

      gcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCaaa                                                                                                                              >  1:540319/1‑140 (MQ=255)

      gcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCaaa                                                                                                                              >  2:1387338/1‑140 (MQ=255)

      gcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCa                                                                                                                                >  1:1643890/1‑138 (MQ=255)

      gcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCa                                                                                                                                <  2:1643890/138‑1 (MQ=255)

      gcACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGCTGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCaaa                                                                                                                              <  1:1307002/140‑1 (MQ=255)

       caccacCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAAc                                                                                                                             >  1:191688/1‑140 (MQ=255)

       caccacCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAAc                                                                                                                             >  2:1309876/1‑140 (MQ=255)

          cacccaccTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGa                                                                                                                          <  2:1570147/140‑1 (MQ=255)

              caccTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTAt                                                                                                                      <  1:2147363/140‑1 (MQ=255)

              caccTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTAt                                                                                                                      <  1:1309876/140‑1 (MQ=255)

                 cTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCAc                                                                                                                   >  2:182882/1‑140 (MQ=255)

                  tCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCAcc                                                                                                                  >  1:492748/1‑140 (MQ=255)

                   cGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCa                                                                                                                 <  2:1469252/140‑1 (MQ=255)

                    ggCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCAt                                                                                                                <  2:2092637/140‑1 (MQ=255)

                     gCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGTCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATc                                                                                                               <  2:702530/140‑1 (MQ=255)

                      ccTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCa                                                                                                              >  1:1905715/1‑140 (MQ=255)

                           cGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGaa                                                                                                         <  2:1040365/140‑1 (MQ=255)

                                   aaCACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATccc                                                                                                 <  1:1205229/140‑1 (MQ=255)

                                    acacCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCg                                                                                                >  1:99513/1‑140 (MQ=255)

                                      acCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              <  1:1970334/140‑1 (MQ=255)

                                      acCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              <  1:475166/140‑1 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              >  2:2072164/1‑138 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              <  1:2072164/138‑1 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGc                                                                                            <  2:1439814/140‑1 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGc                                                                                            >  2:2113916/1‑140 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGc                                                                                            <  1:1928815/140‑1 (MQ=255)

                                        cgtgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGc                                                                                            >  1:1237321/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              >  1:216242/1‑136 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGAc                                                                                              <  2:216242/136‑1 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  1:1053366/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  1:145774/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  2:819705/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  2:166470/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  2:1835832/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  1:32293/1‑140 (MQ=255)

                                          tgcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTAGCTATGTACCAGCACCGTGCGGCAAAGACGCGATTCTGGAAGCCGGCACCGAAGGCATCAATCTGATTATCACCATCACTGAAGGCATCCCGACGCTg                                                                                          >  2:2020944/1‑140 (MQ=255)

                                           gcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTgg                                                                                         <  2:1617880/140‑1 (MQ=255)

                                           gcgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTgg                                                                                         <  2:410399/140‑1 (MQ=255)

                                            cgtgAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGa                                                                                        >  2:1275229/1‑140 (MQ=255)

                                               gAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGttat                                                                                     >  1:2100220/1‑140 (MQ=255)

                                                aaGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATg                                                                                    <  2:1964700/140‑1 (MQ=255)

                                                aaGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATg                                                                                    <  1:2128000/140‑1 (MQ=255)

                                                  gCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCt                                                                                  >  2:1753625/1‑140 (MQ=255)

                                                           gcCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGaa                                                                         >  1:1033634/1‑140 (MQ=255)

                                                              aCTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGt                                                                      <  1:1640056/140‑1 (MQ=255)

                                                               atGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTg                                                                     <  2:540319/139‑1 (MQ=255)

                                                                tGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGa                                                                    >  1:270239/1‑140 (MQ=255)

                                                                  gcgcTACCGCTTCTGTTATCTACGAACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAg                                                                  <  2:70598/140‑1 (MQ=255)

                                                                   cgcTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGc                                                                 <  1:1310042/140‑1 (MQ=255)

                                                                       aCCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGa                                                             >  2:885155/1‑140 (MQ=255)

                                                                        ccGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGAt                                                            >  2:245159/1‑140 (MQ=255)

                                                                                  tATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCg                                                  <  1:1835832/140‑1 (MQ=255)

                                                                                  tATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCg                                                  <  1:2113916/140‑1 (MQ=255)

                                                                                        cGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTa                                            >  1:312082/1‑140 (MQ=255)

                                                                                        cGTACCAACACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTa                                            >  2:1087017/1‑140 (MQ=255)

                                                                                           aCCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGa                                         >  1:1970859/1‑140 (MQ=255)

                                                                                              aGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCg                                      >  1:149068/1‑140 (MQ=255)

                                                                                                cACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGc                                    <  1:2008892/140‑1 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:1358856/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               <  1:921372/140‑1 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  1:1080647/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               <  1:245159/140‑1 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:492/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:1969408/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:1890538/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:592030/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:768562/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  1:1984298/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               <  1:592030/140‑1 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               <  1:768137/140‑1 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:266956/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  1:58272/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  1:1514257/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  1:1515706/1‑140 (MQ=255)

                                                                                                     ttCTGCAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGCGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGaa                               >  2:607485/1‑140 (MQ=255)

                                                                                                          cAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTTAAGCCATCCCGACGCTGGATATGCTGACCGCGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAAATGcc                          <  2:1063204/140‑1 (MQ=255)

                                                                                                          cAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGcc                          >  2:1085301/1‑140 (MQ=255)

                                                                                                          cAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGcc                          <  1:885155/140‑1 (MQ=255)

                                                                                                          cAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGcc                          <  1:1412329/140‑1 (MQ=255)

                                                                                                          cAAAGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGcc                          >  1:1619635/1‑140 (MQ=255)

                                                                                                           aaaGACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGccc                         <  2:145774/140‑1 (MQ=255)

                                                                                                              gACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGTTATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAgg                      <  2:2100220/140‑1 (MQ=255)

                                                                                                              gACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAgg                      <  1:1087017/140‑1 (MQ=255)

                                                                                                              gACTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAgg                      <  2:1997030/140‑1 (MQ=255)

                                                                                                               aCTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGc                     >  2:1405244/1‑140 (MQ=255)

                                                                                                               aCTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGc                     >  2:388387/1‑140 (MQ=255)

                                                                                                               aCTCCATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGc                     >  2:1146223/1‑140 (MQ=255)

                                                                                                                  ccATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGtt                  >  2:983055/1‑140 (MQ=255)

                                                                                                                  ccATTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGtt                  <  2:879856/140‑1 (MQ=255)

                                                                                                                    aTTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTTTGATCGGCCCGAACTGCCCAGGCGTTAt                >  2:2016821/1‑140 (MQ=255)

                                                                                                                    aTTCTGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTAt                <  1:681985/140‑1 (MQ=255)

                                                                                                                        tGGAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACt            >  2:94238/1‑140 (MQ=255)

                                                                                                                         ggAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTc           >  1:380693/1‑140 (MQ=255)

                                                                                                                         ggAAGCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTc           >  2:2136434/1‑140 (MQ=255)

                                                                                                                             gCCATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTCCggg       >  1:352161/1‑140 (MQ=255)

                                                                                                                              ccATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTCCGGGt      <  1:1827149/140‑1 (MQ=255)

                                                                                                                               cATCGACGAAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTCCGGGTg     >  2:472599/1‑140 (MQ=255)

AAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGACTCCATTCTGGAAGCCATCGACGCAGGCATCAAACTGATTATCACCATCACTGAAGGCATCCCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATCGGCCCGAACTGCCCAGGCGTTATCACTCCGGGTGAAT  >  NC_000913/764307‑764576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A