Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,404	G→T	A141S (GCG→TCG)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,404	0	G	T	98.8%	272.0 / ‑3.0	82	A141S (GCG→TCG)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (46/34); total (48/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

CGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTA  >  NC_000913/766270‑766539

cggcgTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCtt                                                                                                                                    >  2:834371/1‑139 (MQ=255)

 ggcgTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGa                                                                                                                                   >  2:64308/1‑140 (MQ=255)

    gTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCa                                                                                                                                <  2:2064482/140‑1 (MQ=255)

     tttGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCAc                                                                                                                               <  2:1746926/140‑1 (MQ=255)

     tttGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCAc                                                                                                                               >  1:372509/1‑140 (MQ=255)

       tGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCAcgc                                                                                                                             <  1:1799287/140‑1 (MQ=255)

           gTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCt                                                                                                                         <  2:372509/140‑1 (MQ=255)

             tgcaacacTCAGCGAGACGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCGATGATGCGGGTTCTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTg                                                                                                                       <  2:1708618/136‑1 (MQ=255)

              gCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGc                                                                                                                      <  2:1191205/140‑1 (MQ=255)

                cacaCTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCgg                                                                                                                    <  2:1644576/140‑1 (MQ=255)

                      cAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGCCACAGCTGACCACGCGCCTTGCGgatgatt                                                                                                              >  2:309931/1‑139 (MQ=255)

                      cAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCt                                                                                                                         <  1:1289824/129‑1 (MQ=255)

                      cAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCt                                                                                                                         >  2:1289824/1‑129 (MQ=255)

                            gCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGga                                                                                                                   <  2:1195186/129‑1 (MQ=255)

                            gCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGga                                                                                                                   >  1:1195186/1‑129 (MQ=255)

                             ccGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCg                                                                                                       <  1:1173996/140‑1 (MQ=255)

                             ccGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCg                                                                                                       >  1:591244/1‑140 (MQ=255)

                             ccGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCg                                                                                                       <  2:555558/140‑1 (MQ=255)

                              cGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCgg                                                                                                      >  1:1410286/1‑140 (MQ=255)

                               gCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                                     <  2:879926/140‑1 (MQ=255)

                               gCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                                     <  1:515454/140‑1 (MQ=255)

                               gCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                                     <  1:1854661/140‑1 (MQ=255)

                               gCTTCAGTGGGAAGGCGTTGATGGCCAGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                                     <  2:2095211/140‑1 (MQ=255)

                                cTTCAGTGGGAAGGCGATGATGACCCAGAAGCAGATGATTTAGTGGTTCTGCTGGAGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGgc                                                                                                    <  2:2058932/140‑1 (MQ=255)

                                 ttCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCg                                                                                                       <  2:575766/136‑1 (MQ=255)

                                 ttCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGgcg                                                                                                   >  1:1875202/1‑140 (MQ=255)

                                 ttCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTCAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCg                                                                                                       >  1:575766/1‑136 (MQ=255)

                                          aaGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACat                                                                                          >  2:863651/1‑139 (MQ=255)

                                           aGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGt                                                                                         >  1:52590/1‑140 (MQ=255)

                                             gCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCg                                                                                       <  2:52590/140‑1 (MQ=255)

                                                ttGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGcg                                                                                    >  1:2067560/1‑140 (MQ=255)

                                                     ggCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCgacgac                                                                               <  2:1875202/140‑1 (MQ=255)

                                                       cccGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGc                                                                             >  2:1455370/1‑140 (MQ=255)

                                                          ggAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCtg                                                                          <  1:834371/140‑1 (MQ=255)

                                                            aaGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCtgat                                                                        <  2:536760/140‑1 (MQ=255)

                                                                aGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGt                                                                    >  2:1781067/1‑140 (MQ=255)

                                                                aGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGt                                                                    >  1:1613901/1‑140 (MQ=255)

                                                                 gTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGtt                                                                   >  2:122242/1‑140 (MQ=255)

                                                                  ttGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTg                                                                  >  2:4339/1‑140 (MQ=255)

                                                                      tttAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGctct                                                              >  2:550484/1‑140 (MQ=255)

                                                                         aGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCgg                                                           <  1:309931/140‑1 (MQ=255)

                                                                          gtggtgCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGgc                                                          <  1:1731468/140‑1 (MQ=255)

                                                                           tggtgCGGCGGGCGATGCCCCGCAATGAAGCGTGTACTAAGCATATGAAACTGCGGGTATGGCTGCCCACGCGCGTTGCGGATGATGGGATTCGGGCGCGTATGCACTAGGCGTCGACGCCTGATGAGTTGCTCTCGgcg                                                         <  2:626634/140‑1 (MQ=255)

                                                                            ggtgCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGgcgc                                                        <  1:1281437/140‑1 (MQ=255)

                                                                              tgctgcTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTg                                                      >  1:931148/1‑140 (MQ=255)

                                                                                ctgctgGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGa                                                    >  2:1534282/1‑140 (MQ=255)

                                                                                ctgctgGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGa                                                    >  2:1861534/1‑140 (MQ=255)

                                                                                ctgctgGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGa                                                    >  1:256454/1‑140 (MQ=255)

                                                                                 tgctgGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGAt                                                   <  1:550484/140‑1 (MQ=255)

                                                                                   ctgGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGa                                                 <  2:2067560/140‑1 (MQ=255)

                                                                                        gATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAgg                                            >  1:2051113/1‑140 (MQ=255)

                                                                                          ttCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGa                                          >  1:343900/1‑140 (MQ=255)

                                                                                           tCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAg                                         >  1:355614/1‑140 (MQ=255)

                                                                                             cccccAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGc                                       <  1:1990853/140‑1 (MQ=255)

                                                                                             cccccAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGc                                       >  1:2026661/1‑140 (MQ=255)

                                                                                                ccAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACg                                    <  2:69885/140‑1 (MQ=255)

                                                                                                 cAATGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGc                                   <  2:1410286/140‑1 (MQ=255)

                                                                                                  aaTGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCa                                  <  1:1455370/140‑1 (MQ=255)

                                                                                                  aaTGAAGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCa                                  <  2:2026661/140‑1 (MQ=255)

                                                                                                       aGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCtt                             >  2:25788/1‑140 (MQ=255)

                                                                                                       aGCGGGTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCtt                             >  1:1101707/1‑140 (MQ=255)

                                                                                                            gTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGcc                        >  2:95007/1‑140 (MQ=255)

                                                                                                            gTACTACGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGcc                        <  2:256454/140‑1 (MQ=255)

                                                                                                                  cGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCtttt                  >  1:461416/1‑140 (MQ=255)

                                                                                                                  cGCATATGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCtttt                  >  2:2016730/1‑140 (MQ=255)

                                                                                                                      tatGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCa              >  1:1357440/1‑140 (MQ=255)

                                                                                                                      tatGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCa              >  2:1101026/1‑140 (MQ=255)

                                                                                                                      tatGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCa              >  1:2141754/1‑140 (MQ=255)

                                                                                                                      tatGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCa              <  1:25788/140‑1 (MQ=255)

                                                                                                                      tatGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCa              >  2:589721/1‑140 (MQ=255)

                                                                                                                       atGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCaa             >  2:1164697/1‑140 (MQ=255)

                                                                                                                       atGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCaa             >  2:643296/1‑140 (MQ=255)

                                                                                                                        tGCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAAc            <  2:1452435/140‑1 (MQ=255)

                                                                                                                         gCAACTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAAcg           >  2:1735020/1‑140 (MQ=255)

                                                                                                                            aCTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc        >  1:415507/1‑140 (MQ=255)

                                                                                                                            aCTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc        >  1:1830094/1‑140 (MQ=255)

                                                                                                                            aCTGCTGACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc        >  2:2007536/1‑140 (MQ=255)

                                                                                                                             ctgctgACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc        >  2:1051122/1‑139 (MQ=255)

                                                                                                                             ctgctgACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc        <  1:1051122/139‑1 (MQ=255)

                                                                                                                             ctgctgACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCATCTTTTTCCAACGCGcc       >  1:1896889/1‑140 (MQ=255)

                                                                                                                               gctgACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCaa     >  2:1000788/1‑140 (MQ=255)

                                                                                                                                ctgACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAAc    >  1:1623376/1‑140 (MQ=255)

                                                                                                                                  gACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTa  >  2:1793154/1‑140 (MQ=255)

                                                                                                                                  gACATCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTa  >  2:815033/1‑140 (MQ=255)

                                                                                                                                  gACAGCGCTGACCACGCGCCTTGCGGATGATGATATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTa  >  2:1902313/1‑140 (MQ=255)

CGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTA  >  NC_000913/766270‑766539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A