Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	782,216	G→T	M44I (ATG→ATT)	nadA →	quinolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	782,216	0	G	T	99.1%	371.5 / ‑3.5	109	M44I (ATG→ATT)	nadA	quinolinate synthase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (39/69); total (39/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 112 total aligned reads displayed.

AGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTGAGAT  >  NC_000913/782083‑782352

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTGCCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1016970/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGAttgttgtc                                                                                                                                    <  2:1152915/140‑3 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:626889/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:1135029/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:613303/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1954916/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1954403/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:1120996/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1767313/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1755849/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1689659/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1676374/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:735811/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:118335/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1491169/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:75575/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:865850/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1130992/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1137960/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:355211/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:641703/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1421380/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:516463/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:1282544/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:823216/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1488923/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCAAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  1:1595013/140‑1 (MQ=255)

aGATGAGCGTAATGTTTGAGCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGcc                                                                                                                                    <  2:59179/140‑1 (MQ=255)

 gATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGccc                                                                                                                                   <  1:1701632/140‑1 (MQ=255)

 gATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGccc                                                                                                                                   <  2:853486/140‑1 (MQ=255)

 gATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGccc                                                                                                                                   <  1:74381/140‑1 (MQ=255)

 gATGAGCGTAATGTATGATCCAGATACGGCGATTTATCCTTTCCCCCCGAAGCCGGTGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAACCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGccc                                                                                                                                   <  1:2092986/140‑1 (MQ=255)

  aTGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCa                                                                                                                                  <  1:1826388/140‑1 (MQ=255)

  aTGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCa                                                                                                                                  <  2:140578/140‑1 (MQ=255)

   tGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCac                                                                                                                                 <  1:1948476/140‑1 (MQ=255)

       cGTAATGTTTGATCCAGACACGGTGATTTATCCTTTCCCCCAGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGTGGTGATTGTTGCCCactact                                                                                                                             >  2:1360186/1‑140 (MQ=255)

                tGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATc                                                                                                                    >  1:1997741/1‑140 (MQ=255)

                 gATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATcc                                                                                                                   >  2:408427/1‑140 (MQ=255)

                 gATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATcc                                                                                                                   >  1:722408/1‑140 (MQ=255)

                 gATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATcc                                                                                                                   >  2:306953/1‑140 (MQ=255)

                    ccAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGa                                                                                                                <  2:1568613/140‑1 (MQ=255)

                      aGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGaaa                                                                                                              >  2:2121736/1‑140 (MQ=255)

                        acacGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAAtt                                                                                                            >  1:1248824/1‑140 (MQ=255)

                        acacGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAAtt                                                                                                            >  1:67558/1‑140 (MQ=255)

                            ggCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaac                                                                                                        >  1:342910/1‑140 (MQ=255)

                            ggCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaac                                                                                                        >  1:2110541/1‑140 (MQ=255)

                            ggCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaac                                                                                                        >  1:1290362/1‑140 (MQ=255)

                             gCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaaca                                                                                                       <  1:306953/140‑1 (MQ=255)

                             gCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaaca                                                                                                       <  1:408427/140‑1 (MQ=255)

                              cGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTcaacaa                                                                                                      >  1:285072/1‑140 (MQ=255)

                                 tttATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTg                                                                                                   >  2:1530064/1‑140 (MQ=255)

                                  ttATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTgg                                                                                                  <  1:404031/140‑1 (MQ=255)

                                          tCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAgaagaa                                                                                          >  2:734053/1‑140 (MQ=255)

                                          tCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAgaagaa                                                                                          >  1:1142839/1‑140 (MQ=255)

                                              cccGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCg                                                                                      <  2:1142839/140‑1 (MQ=255)

                                               ccGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCgg                                                                                     >  2:868719/1‑140 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    <  2:1057879/140‑1 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    <  1:511069/140‑1 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    <  2:722408/140‑1 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    <  2:1215578/140‑1 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    >  1:1437789/1‑140 (MQ=255)

                                                cGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGt                                                                                    <  1:1459695/140‑1 (MQ=255)

                                                    gCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCt                                                                                <  2:1886812/140‑1 (MQ=255)

                                                     ccGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTg                                                                               <  2:2080813/140‑1 (MQ=255)

                                                      cGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGt                                                                              >  1:1494936/1‑140 (MQ=255)

                                                      agacgacGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATTCCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGt                                                                              <  2:1726216/139‑1 (MQ=255)

                                                        aCGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTAt                                                                            <  1:949428/140‑1 (MQ=255)

                                                        aCGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTAt                                                                            >  1:264510/1‑140 (MQ=255)

                                                               tAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGAt                                                                     >  1:1478289/1‑140 (MQ=255)

                                                               tAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGAt                                                                     <  2:2110541/140‑1 (MQ=255)

                                                                        atgaAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGaa                                                            >  1:588926/1‑140 (MQ=255)

                                                                        atgaAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGaa                                                            >  2:1556200/1‑140 (MQ=255)

                                                                        atgaAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGaa                                                            >  1:304792/1‑140 (MQ=255)

                                                                                   tATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCtt                                                 <  2:2010365/140‑1 (MQ=255)

                                                                                     ttACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCg                                               >  2:1591215/1‑140 (MQ=255)

                                                                                           cgAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGaaa                                                           <  1:525333/122‑1 (MQ=255)

                                                                                           cgAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGaaa                                                           >  2:525333/1‑122 (MQ=255)

                                                                                                  aTAAAACGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATcc                                  <  2:1478289/140‑1 (MQ=255)

                                                                                                    aaaaCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCg                                <  1:1556200/140‑1 (MQ=255)

                                                                                                     aaaCGACTGCTAAAAGAACGTAAGGCGGTGATTGTTGCCAACTACTATACCAAGCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGc                               <  2:2027182/140‑1 (MQ=255)

                                                                                                      aaCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCt                              <  2:285072/140‑1 (MQ=255)

                                                                                                      aaCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCt                              <  1:2121736/140‑1 (MQ=255)

                                                                                                      aaCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCt                              <  1:1105125/140‑1 (MQ=255)

                                                                                                       aCGTCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCtt                             <  2:1248824/140‑1 (MQ=255)

                                                                                                          tCTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTa                          >  1:811319/1‑140 (MQ=255)

                                                                                                          cttGCTAAAAGAACGTATGCCGGTGATTTTGCCCCACAACTATACCGATCCCGAAATTCAACAACCGGCAGAGGAAACCGGTGGCTGTATTTCTGATTCTCGGGAGATGGCGCGCTTCGGTGCAAAGCATCCGCCTTCtt                          >  2:1121132/3‑139 (MQ=255)

                                                                                                           cTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTAc                         >  2:1095333/1‑140 (MQ=255)

                                                                                                           cTGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTAc                         >  1:144332/1‑140 (MQ=255)

                                                                                                            tGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACt                        <  2:304792/140‑1 (MQ=255)

                                                                                                            tGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACt                        >  2:1190003/1‑140 (MQ=255)

                                                                                                            tGCTAAAAGAACGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACt                        <  1:1478094/140‑1 (MQ=255)

                                                                                                                       cGTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCGGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGc             >  2:212260/1‑140 (MQ=255)

                                                                                                                        gTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTc                            <  2:1801792/124‑1 (MQ=255)

                                                                                                                        gTAATGCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCt            <  2:144332/140‑1 (MQ=255)

                                                                                                                             gCGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGt       <  1:1591215/140‑1 (MQ=255)

                                                                                                                              cGGTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTg      <  1:2063801/140‑1 (MQ=255)

                                                                                                                               ggTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTga     >  2:1378682/1‑140 (MQ=255)

                                                                                                                               ggTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTga     >  1:1121192/1‑140 (MQ=255)

                                                                                                                                gTGATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTgag    >  1:663388/1‑140 (MQ=255)

                                                                                                                                  gATTGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTGAGAt  <  2:342910/140‑1 (MQ=255)

AGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCGAAATTCAACAACTGGCAGAAGAAACCGGTGGCTGTATTTCTGATTCTCTGGAAATGGCGCGCTTCGGTGCAAAGCATCCCGCTTCTACTTTGTTAGTCGCTGGGGTGAGAT  >  NC_000913/782083‑782352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A