Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,891	C→A	A192A (GCG→GCT)	focA ←	formate channel FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,891	0	C	A	99.3%	484.0 / ‑4.8	141	A192A (GCG→GCT)	focA	formate channel FocA
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (66/74); total (66/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

CAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTCGGCGGTTTGTAGGA  >  NC_000913/953755‑954021

        aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCAt                                                                                                                         >  1:426029/1‑140 (MQ=255)

        aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCAt                                                                                                                         >  2:2049867/1‑140 (MQ=255)

        aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCAt                                                                                                                         >  2:1210292/1‑140 (MQ=255)

        aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCAt                                                                                                                         >  2:1231359/1‑140 (MQ=255)

        aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCAt                                                                                                                         >  2:125198/1‑140 (MQ=255)

            cGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAgg                                                                                                                     <  1:1231359/140‑1 (MQ=255)

            cGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAgg                                                                                                                     >  2:1744259/1‑140 (MQ=255)

            cGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAgg                                                                                                                     >  1:1776395/1‑140 (MQ=255)

             ggTCCAACATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGTTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGc                                                                                                                    >  2:1155555/1‑140 (MQ=255)

             ggTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGc                                                                                                                    >  2:462855/1‑140 (MQ=255)

              gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCCTCAGGCt                                                                                                                   <  2:2018519/140‑1 (MQ=255)

              gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCt                                                                                                                   <  1:1417412/140‑1 (MQ=255)

               tCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCt                                                                                                                   <  1:1131883/139‑1 (MQ=255)

               tCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCt                                                                                                                   >  2:1131883/1‑139 (MQ=255)

                   aaaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGc                                                                                                              <  2:1996662/140‑1 (MQ=255)

                   aaaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGc                                                                                                              <  1:1283733/140‑1 (MQ=255)

                   aaaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGc                                                                                                              <  1:118214/140‑1 (MQ=255)

                   aaaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGc                                                                                                              <  2:892667/140‑1 (MQ=255)

                   aaaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGc                                                                                                              <  1:895150/140‑1 (MQ=255)

                    aaTTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGcc                                                                                                             <  1:579428/140‑1 (MQ=255)

                      ttCAGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAg                                                                                                           <  1:111551/140‑1 (MQ=255)

                       tCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGa                                                                                                          <  1:1965012/140‑1 (MQ=255)

                         cGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCgg                                                                                                               <  1:1360099/133‑1 (MQ=255)

                         cGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCgg                                                                                                               >  2:1360099/1‑133 (MQ=255)

                         cGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAAt                                                                                                        <  1:1155555/140‑1 (MQ=255)

                           gggATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATaa                                                                                                      <  2:525554/140‑1 (MQ=255)

                           gggATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATaa                                                                                                      <  1:1547751/140‑1 (MQ=255)

                                 cGAAGTCGCGGATTACAATACCCATCGGGTTCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAAGCTGCGGCCAGAATAACTCATc                                                                                                >  2:1652327/1‑140 (MQ=255)

                                   aaGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCa                                                                                              <  1:462855/140‑1 (MQ=255)

                                   aaGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCa                                                                                              <  1:1271634/140‑1 (MQ=255)

                                        gcgGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTg                                                                                         <  2:365498/140‑1 (MQ=255)

                                              tACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGAc                                                                                   <  1:341533/140‑1 (MQ=255)

                                              tACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGAc                                                                                   <  2:1894207/140‑1 (MQ=255)

                                               aCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACa                                                                                  <  2:1349742/140‑1 (MQ=255)

                                               aCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACa                                                                                  <  1:1496490/140‑1 (MQ=255)

                                                     accATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCAt                                                                            <  2:719157/139‑1 (MQ=255)

                                                      ccATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATc                                                                           >  1:219402/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAGCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGc                                                                                        >  2:174240/1‑125 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  1:830260/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  2:948353/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  2:498306/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  2:468014/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  1:1574560/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAg                                                                         >  1:1384626/1‑140 (MQ=255)

                                                        aTCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCCGGCAACAAACATCGCGTCCGGCACCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCATACATACCATCAg                                                                         >  1:1357095/1‑140 (MQ=255)

                                                         tCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAgg                                                                        <  1:542866/140‑1 (MQ=255)

                                                          cGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGt                                                                       >  1:999302/1‑140 (MQ=255)

                                                          cGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAAAATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGt                                                                       >  2:1085925/1‑140 (MQ=255)

                                                          cGGGATCATAAACATGTTTGCGATACTGTGCTAAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGt                                                                       >  2:1159670/1‑140 (MQ=255)

                                                           gggATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGtt                                                                      >  2:365432/1‑140 (MQ=255)

                                                            ggATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGttt                                                                     <  1:919426/140‑1 (MQ=255)

                                                               tCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGcc                                                                  >  2:1614403/1‑140 (MQ=255)

                                                                 aTAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCTTCAGGTTTGCCAg                                                                <  2:2052157/140‑1 (MQ=255)

                                                                     aCATGTTTGCGATACTGTGCTAAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATa                                                            <  1:1159670/140‑1 (MQ=255)

                                                                      cATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATAc                                                           <  1:399567/140‑1 (MQ=255)

                                                                      cATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATAc                                                           <  2:1384626/140‑1 (MQ=255)

                                                                        tGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGTCAGGATACCa                                                         >  1:1978958/1‑140 (MQ=255)

                                                                        tGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCa                                                         >  2:1322842/1‑140 (MQ=255)

                                                                         gTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCaa                                                        >  1:1082534/1‑140 (MQ=255)

                                                                         gTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCaa                                                        <  2:129557/140‑1 (MQ=255)

                                                                          tttGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAg                                                                >  1:1638657/1‑131 (MQ=255)

                                                                          tttGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAg                                                                <  2:1638657/131‑1 (MQ=255)

                                                                          gttGCGATACTGGGCTCAAAACCGCTGCCAACAAACATCGCGACCGGCAGCACCAAAATCAAAGCTTGGACCATCAGGCTACGCCCAGAATAACTCATTCATACTGCCAGACATACCCTCAGGTTTTCCAGGAAACCAag                                                       >  2:711403/2‑140 (MQ=255)

                                                                           ttGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAaga                                                      >  2:2115339/1‑140 (MQ=255)

                                                                           ttGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAaga                                                      >  1:1185320/1‑140 (MQ=255)

                                                                            tGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAagac                                                     >  1:896804/1‑140 (MQ=255)

                                                                                aTACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAagacagac                                                 >  2:615368/1‑140 (MQ=255)

                                                                                   cTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              >  1:1937256/1‑140 (MQ=255)

                                                                                   cTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              <  2:219402/140‑1 (MQ=255)

                                                                                   cTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              >  1:343194/1‑140 (MQ=255)

                                                                                   cTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              <  1:211162/140‑1 (MQ=255)

                                                                                   atgtgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              <  2:535100/139‑1 (MQ=255)

                                                                                    tgtgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAagacaga                                                  <  1:1469314/135‑1 (MQ=255)

                                                                                     gtgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCt                                            <  1:1814403/140‑1 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              >  1:1398212/1‑137 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              <  2:1398212/137‑1 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:1511660/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:146712/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  1:273268/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:1419819/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:1017187/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:876533/1‑140 (MQ=255)

                                                                                      tgCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTc                                           >  2:317960/1‑140 (MQ=255)

                                                                                       gCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCa                                          <  2:1954371/140‑1 (MQ=255)

                                                                                        cTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCaa                                         <  1:2115339/140‑1 (MQ=255)

                                                                                         tCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAAt                                        >  1:131546/1‑140 (MQ=255)

                                                                                             aaCCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATaaaa                                    <  1:125198/140‑1 (MQ=255)

                                                                                             aaCCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATaaaa                                    <  1:1638193/140‑1 (MQ=255)

                                                                                                cGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTa                                 >  1:1438470/1‑140 (MQ=255)

                                                                                                cGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTa                                 >  2:1926096/1‑140 (MQ=255)

                                                                                                  cTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGc                                              >  2:864097/1‑125 (MQ=255)

                                                                                                  cTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATg                               >  1:751717/1‑140 (MQ=255)

                                                                                                    ggCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGt                             <  1:1744259/140‑1 (MQ=255)

                                                                                                     gCAACAAACATCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTg                            <  1:1926096/140‑1 (MQ=255)

                                                                                                              aTCGCGACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTtgtg                   <  1:876533/140‑1 (MQ=255)

                                                                                                                cgcgACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGt                 <  1:985513/140‑1 (MQ=255)

                                                                                                                  cgACCGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCTTCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTcg               <  2:1786157/140‑1 (MQ=255)

                                                                                                                     ccGGCAGCACCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGTCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTcggcg            <  2:2089436/140‑1 (MQ=255)

                                                                                                                           gcaCCATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTCGGCGGTTTGt      >  2:66379/1‑140 (MQ=255)

                                                                                                                              ccATAATGAAAGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTCGGCGGTTTGTAgg   <  2:1786978/140‑1 (MQ=255)

CAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTGTCCATCAGGCTGCGGCCAGAATAACTCATCCATACTGCCAGACATACCATCAGGTTTGCCAGGATACCAAGACAGACGGCCTCAATAAAAGTATGGTGCACTTTGTGGTCGGCGGTTTGTAGGA  >  NC_000913/953755‑954021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A