Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	964,264	C→A	intergenic (+152/‑56)	ihfB → / → ycaI	integration host factor subunit beta/conserved inner membrane protein YcaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	964,264	0	C	A	98.9%	310.3 / NA	92	intergenic (+152/‑56)	ihfB/ycaI	integration host factor subunit beta/conserved inner membrane protein YcaI
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (39/52); total (39/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGG  >  NC_000913/964136‑964394

gagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACaa                                                                                                                         >  2:2034415/1‑140 (MQ=255)

gagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACaa                                                                                                                         >  2:1226251/1‑140 (MQ=255)

gagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACaa                                                                                                                         >  1:1477363/1‑140 (MQ=255)

  gaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAAcc                                                                                                                       >  2:1349159/1‑140 (MQ=255)

   aaaaGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCg                                                                                                                      <  2:265722/140‑1 (MQ=255)

   aaaaGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCg                                                                                                                      <  2:1378041/140‑1 (MQ=255)

          ccTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTcaaca                                                                                                               <  2:2028905/140‑1 (MQ=255)

          ccTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTcaaca                                                                                                               <  2:493460/140‑1 (MQ=255)

          ccTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTcaaca                                                                                                               <  2:504151/140‑1 (MQ=255)

           cTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTcaacaa                                                                                                              >  1:1636794/1‑140 (MQ=255)

             gTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAg                                                                                                            >  2:379278/1‑140 (MQ=255)

                gggTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGttt                                                                                                         >  2:1019694/1‑139 (MQ=255)

                gggTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTg                                                                                                         >  1:46678/1‑140 (MQ=255)

                 ggTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGc                                                                                                        <  2:46678/140‑1 (MQ=255)

                  gTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGca                                                                                                       <  2:750450/140‑1 (MQ=255)

                  gTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGca                                                                                                       >  1:1499965/1‑140 (MQ=255)

                    gCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGcaca                                                                                                     <  1:13977/140‑1 (MQ=255)

                    gCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGcaca                                                                                                     <  1:344418/140‑1 (MQ=255)

                     cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCacac                                                                                                    <  2:1757860/140‑1 (MQ=255)

                      ttttttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACt                                                                                                   <  1:1175606/140‑1 (MQ=255)

                        ttttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACttt                                                                                                 >  1:213239/1‑140 (MQ=255)

                         tttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTc                                                                                                <  2:1956000/140‑1 (MQ=255)

                         tttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTGTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTc                                                                                                <  2:546720/140‑1 (MQ=255)

                           tCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCa                                                                                              <  1:111662/140‑1 (MQ=255)

                              tttCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATaa                                                                                           <  1:2034415/140‑1 (MQ=255)

                               ttCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATaaa                                                                                          >  1:299421/1‑140 (MQ=255)

                                tctcTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAAc                                                                                         >  1:137/1‑140 (MQ=255)

                                   ctAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACagg                                                                                      <  1:1147902/140‑1 (MQ=255)

                                    tAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACaggg                                                                                     <  2:1477363/140‑1 (MQ=255)

                                    tAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACC‑CCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACaggga                                                                                    >  1:828195/1‑140 (MQ=255)

                                      aTCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACagggag                                                                                   <  1:992992/140‑1 (MQ=255)

                                         tggaactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAgggg                                                                                >  2:2088868/1‑140 (MQ=255)

                                           gaactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTg                                                                              >  2:1270089/1‑140 (MQ=255)

                                           gaactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTg                                                                              >  2:1919152/1‑140 (MQ=255)

                                           gaactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTg                                                                              >  2:244726/1‑140 (MQ=255)

                                            aactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGc                                                                             >  1:556414/1‑140 (MQ=255)

                                               tggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATg                                                                          <  1:1339485/140‑1 (MQ=255)

                                               tggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATg                                                                          <  2:1952404/140‑1 (MQ=255)

                                               tggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATg                                                                          <  1:1244337/140‑1 (MQ=255)

                                                ggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGa                                                                         >  2:2051323/1‑140 (MQ=255)

                                                 gaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGaa                                                                        >  2:814720/1‑140 (MQ=255)

                                                 gaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGaa                                                                        >  2:622790/1‑140 (MQ=255)

                                                    gCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAAt                                                                     <  2:1564594/140‑1 (MQ=255)

                                                     cTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATa                                                                    <  1:232642/140‑1 (MQ=255)

                                                       gCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAAc                                                                  <  2:1636794/140‑1 (MQ=255)

                                                        ccTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACg                                                                 >  1:1844998/1‑140 (MQ=255)

                                                        ccTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACg                                                                 >  2:1716976/1‑140 (MQ=255)

                                                         cTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCGCCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGa                                                                <  2:299421/140‑1 (MQ=255)

                                                         cTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGa                                                                <  1:1716976/140‑1 (MQ=255)

                                                         cTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGa                                                                <  1:1156404/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1292574/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1848019/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1286235/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  2:1499965/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1226251/140‑1 (MQ=255)

                                                             cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1089490/140‑1 (MQ=255)

                                                             aagagTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  2:878180/139‑1 (MQ=255)

                                                              agagTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAAGAGGGAGGGGTGCATGAAAATAACGACAGTc                                                           <  1:1349159/140‑1 (MQ=255)

                                                              agagTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTc                                                           <  1:142231/140‑1 (MQ=255)

                                                              agagTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTc                                                           <  2:1844998/140‑1 (MQ=255)

                                                                agTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCgg                                                         <  2:374139/140‑1 (MQ=255)

                                                                 gTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgt                                                        <  2:1899277/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  2:987637/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  2:137/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  1:1163586/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  1:1270089/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  1:622790/140‑1 (MQ=255)

                                                                  ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  1:1887244/140‑1 (MQ=255)

                                                                    ttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       >  2:1213597/1‑138 (MQ=255)

                                                                    ttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                       <  1:1213597/138‑1 (MQ=255)

                                                                          aGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAg                                                             >  2:1588930/1‑126 (MQ=255)

                                                                          aGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAg                                                             <  1:1588930/126‑1 (MQ=255)

                                                                            ttttCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            >  2:1920946/1‑125 (MQ=255)

                                                                            ttttCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAAt                                             >  1:169189/1‑140 (MQ=255)

                                                                            ttttCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAATTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                            <  1:1920946/125‑1 (MQ=255)

                                                                              ttCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTa                                           >  2:697739/1‑140 (MQ=255)

                                                                               tCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAg                                          <  2:1625997/140‑1 (MQ=255)

                                                                                      ttCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAAtt                                   >  1:1116306/1‑140 (MQ=255)

                                                                                      ttCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAAtt                                   >  2:502085/1‑140 (MQ=255)

                                                                                      ttCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAAtt                                   >  2:2103357/1‑140 (MQ=255)

                                                                                      ttCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAAtt                                   >  1:846852/1‑140 (MQ=255)

                                                                                            aaaTTCTTCTGAATATGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTcct                             >  2:203368/1‑139 (MQ=255)

                                                                                                         tatGCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGc                >  1:7573/1‑140 (MQ=255)

                                                                                                            gCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCTTAATTAGCGGAATTTTTCCGTTGCTGATTTTGcccc             >  1:1603862/1‑140 (MQ=255)

                                                                                                            gCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGcccc             >  1:1718267/1‑140 (MQ=255)

                                                                                                            gCCTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGcccc             >  1:1359268/1‑140 (MQ=255)

                                                                                                              cTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCaa           <  1:2051323/140‑1 (MQ=255)

                                                                                                              cTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCaa           <  1:379278/140‑1 (MQ=255)

                                                                                                              cTCGGGGAACGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCaa           <  2:1143872/140‑1 (MQ=255)

                                                                                                                     aaCGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTg    <  1:1919152/140‑1 (MQ=255)

                                                                                                                     aaCGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTg    <  2:130765/140‑1 (MQ=255)

                                                                                                                      aCGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTgg   >  2:98596/1‑140 (MQ=255)

                                                                                                                       cGCAAAATTACCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTggg  >  1:1857393/1‑140 (MQ=255)

GAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGG  >  NC_000913/964136‑964394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A