Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F48 I1 R1 61 74.3 2613450 99.1% 2589928 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 979,123 C→A P933Q (CCG→CAG)  mukB → chromosome partitioning protein MukB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913979,1230CA100.0% 189.7 / NA 53P933Q (CCG→CAG) mukBchromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/33);  total (20/33)

GATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATGC  >  NC_000913/978987‑979260
                                                                                                                                         |                                                                                                                                         
gatgaCAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGa                                                                                                                                        <  2:473364/140‑1 (MQ=255)
gatgaCAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGa                                                                                                                                        <  2:847683/140‑1 (MQ=255)
gatgaCAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGa                                                                                                                                        <  2:1113124/140‑1 (MQ=255)
       gCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTc                                                                                                                                 >  2:1265741/1‑140 (MQ=255)
       gCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTc                                                                                                                                 >  1:82857/1‑140 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  2:1123456/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  2:882623/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  1:982571/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  1:417374/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  2:159213/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  1:51828/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  1:555803/140‑1 (MQ=255)
          tGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGaa                                                                                                                              <  2:83370/140‑1 (MQ=255)
             cGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACat                                                                                                                           >  2:676189/1‑139 (MQ=255)
              ggATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGt                                                                                                                          <  1:758080/140‑1 (MQ=255)
                 tCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTaa                                                                                                                       <  2:82857/140‑1 (MQ=255)
                    cgTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAaag                                                                                                                    <  2:468972/140‑1 (MQ=255)
                    cgTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAaag                                                                                                                    <  2:37377/140‑1 (MQ=255)
                    cgTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAaag                                                                                                                    <  2:703766/140‑1 (MQ=255)
                        gATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAagaaga                                                                                                                <  1:766341/140‑1 (MQ=255)
                         aTGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGAt                                                                                                               <  1:1103767/140‑1 (MQ=255)
                              aTCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTAcgc                                                                                                          <  2:881111/140‑1 (MQ=255)
                              aTCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTAcgc                                                                                                          <  1:1265741/140‑1 (MQ=255)
                               tCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTAcgcg                                                                                                         <  2:808567/140‑1 (MQ=255)
                               tCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTAcgcg                                                                                                         <  2:384691/140‑1 (MQ=255)
                                    gAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTActc                                                                                                    <  1:676189/140‑1 (MQ=255)
                                    gAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTActc                                                                                                    <  2:1253537/140‑1 (MQ=255)
                                                  aGCACAAGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCAAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGTACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAgc                                                                                      >  2:156850/1‑140 (MQ=255)
                                                      cAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGa                                                                                  <  2:112985/140‑1 (MQ=255)
                                                                  cgTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTcagca                                                                      <  1:297104/140‑1 (MQ=255)
                                                                            agcagTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGcc                                                            <  1:1262540/140‑1 (MQ=255)
                                                                                 tttGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGAc                                                       >  1:438268/1‑140 (MQ=255)
                                                                                 tttGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGAc                                                       >  1:1279677/1‑140 (MQ=255)
                                                                                  ttGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACg                                                      >  1:972734/1‑140 (MQ=255)
                                                                                   tGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAACAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACgg                                                     >  1:1269125/1‑140 (MQ=255)
                                                                                      cAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAg                                                  <  2:972734/140‑1 (MQ=255)
                                                                                        aTCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAgtg                                                >  1:965136/1‑140 (MQ=255)
                                                                                           aaCTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGTGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAgtggtg                                             <  2:926582/140‑1 (MQ=255)
                                                                                              tGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAATGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAg                                          >  2:1087178/1‑140 (MQ=255)
                                                                                                     aCTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTg                                   >  1:564531/1‑140 (MQ=255)
                                                                                                     aCTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAACAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTg                                   >  2:927956/1‑140 (MQ=255)
                                                                                                       tGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTgcg                                 <  2:860766/140‑1 (MQ=255)
                                                                                                        ggAACCGATTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTgcgc                                <  1:229801/140‑1 (MQ=255)
                                                                                                               aTTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAg                         >  1:1007745/1‑140 (MQ=255)
                                                                                                               aTTGTTTC‑GGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAg                         >  1:75294/1‑140 (MQ=255)
                                                                                                                atGTTTCGGGTATTGCAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACTCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAg                         <  1:429096/139‑1 (MQ=255)
                                                                                                                 tGTTTC‑GGTATTGCAGAGCGACCAGGAAGAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCt                       >  1:930928/1‑140 (MQ=255)
                                                                                                                               cAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTAt                     >  2:741701/1‑129 (MQ=255)
                                                                                                                               cAGAGCGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTAt                     <  1:741701/129‑1 (MQ=255)
                                                                                                                                    cGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGNAGCNCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGaaa     >  2:1170581/1‑140 (MQ=255)
                                                                                                                                    cGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGaaa     >  2:1100612/1‑140 (MQ=255)
                                                                                                                                    cGACCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGaaa     >  2:1212447/1‑140 (MQ=255)
                                                                                                                                      aCCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATg   >  1:187957/1‑140 (MQ=255)
                                                                                                                                      aCCAGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATg   >  1:595340/1‑140 (MQ=255)
                                                                                                                                       cccGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATGc  >  2:936857/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GATGACAGCCTGGCGGATCGCGTCGATGAAATCCGCGAACGTCTGGATGAAGCCCAGGAAGCCGCGCGTTTTGTTCAGCAGTTTGGCAATCAACTGGCGAAACTGGAACCGATTGTTTC‑GGTATTGCAGAGCGACCCGGAACAGTTCGAACAGTTAAAAGAAGATTACGCGTACTCTCAGCAGATGCAGCGCGATGCCCGTCAGCAGGCGTTTGCCCTGACGGAAGTGGTGCAGCGTCGTGCGCACTTTAGCTATTCTGACTCGGCAGAAATGC  >  NC_000913/978987‑979260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A