Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F48 I1 R1 45 83.3 2696702 99.2% 2675128 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 580,273 G→T S58* (TCA→TAA)  ylcI ← DLP12 prophage; DUF3950 domain‑containing protein YlcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,2730GT98.5% 242.3 / ‑2.8 68S58* (TCA→TAA) ylcIDLP12 prophage; DUF3950 domain‑containing protein YlcI
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (42/25);  total (43/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

CAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGT  >  NC_000913/580140‑580412
                                                                                                                                     |                                                                                                                                           
cAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGtat                                                                                                                                       >  1:1140092/1‑140 (MQ=255)
cAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGtat                                                                                                                                       <  1:464580/140‑1 (MQ=255)
 aGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGtata                                                                                                                                      >  2:476213/1‑140 (MQ=255)
  gTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTatat                                                                                                                                     >  2:652854/1‑140 (MQ=255)
    ctgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGc                                                                                                                                   >  1:707101/1‑140 (MQ=255)
    ctgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGc                                                                                                                                   <  1:121630/140‑1 (MQ=255)
     tgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  <  1:652854/140‑1 (MQ=255)
     tgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  >  2:739596/1‑140 (MQ=255)
     tgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  <  2:713741/140‑1 (MQ=255)
     tgtcactgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGAGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  <  2:480595/140‑1 (MQ=255)
         actgtcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGctctct                                                                                                                              >  1:426148/1‑140 (MQ=255)
             tcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  >  1:10064/1‑132 (MQ=255)
             tcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCttttt                                                                                                                          >  1:1015000/1‑139 (MQ=255)
             tcaGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGACCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGAGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGct                                                                                                                                  <  2:10064/132‑1 (MQ=255)
               aGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTg                                                                                                                        >  2:1254097/1‑140 (MQ=255)
                 gAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGAc                                                                                                                      >  1:177578/1‑140 (MQ=255)
                       ggTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGc                                                                                                                >  1:1155425/1‑140 (MQ=255)
                       ggTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGc                                                                                                                >  1:757027/1‑140 (MQ=255)
                       ggTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGc                                                                                                                >  1:74810/1‑140 (MQ=255)
                       ggTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGc                                                                                                                >  2:808934/1‑140 (MQ=255)
                                gCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACgg                                                                                                       <  1:238579/140‑1 (MQ=255)
                                 cAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGc                                                                                                      <  1:808934/140‑1 (MQ=255)
                                 aaaCTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGc                                                                                                      <  2:426148/139‑1 (MQ=255)
                                  aaCTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACgaca                                                                                                     <  1:725036/140‑4 (MQ=255)
                                         ttACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCa                                                                                              >  1:89062/1‑140 (MQ=255)
                                                aaTGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGAccc                                                                                       <  2:1155425/140‑1 (MQ=255)
                                                 aTGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCa                                                                                      >  1:543124/1‑140 (MQ=255)
                                                 aTGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCa                                                                                      >  1:197025/1‑140 (MQ=255)
                                                    cccTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGc                                                                                   >  1:799524/1‑140 (MQ=255)
                                                     ccTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCt                                                                                  <  1:454600/140‑1 (MQ=255)
                                                       tCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTga                                                                                >  2:168217/1‑140 (MQ=255)
                                                           aaTTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGaaa                                                                            <  1:1158407/140‑1 (MQ=255)
                                                                   gAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGTCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGa                                                                    >  2:326136/1‑140 (MQ=255)
                                                                   gAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGa                                                                    >  1:1152035/1‑140 (MQ=255)
                                                                         aCAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCttt                                                              <  2:177578/140‑1 (MQ=255)
                                                                               tCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTc                                                        >  2:593781/1‑140 (MQ=255)
                                                                                  tCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAag                                                     >  2:1098479/1‑140 (MQ=255)
                                                                                  tCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAag                                                     >  1:780842/1‑140 (MQ=255)
                                                                                  tCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAag                                                     >  2:951610/1‑140 (MQ=255)
                                                                                    cTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAagag                                                   <  1:476213/140‑1 (MQ=255)
                                                                                    cTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAagag                                                   <  1:268245/140‑1 (MQ=255)
                                                                                     tGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGc                                                  >  2:878802/1‑140 (MQ=255)
                                                                                      gTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCg                                                 <  1:168217/140‑1 (MQ=255)
                                                                                       ttCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGa                                                >  2:781654/1‑140 (MQ=255)
                                                                                       ttCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGa                                                >  2:1172359/1‑140 (MQ=255)
                                                                                       ttCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGa                                                >  1:521813/1‑140 (MQ=255)
                                                                                            aGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTa                                           >  1:565879/1‑140 (MQ=255)
                                                                                            aGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTa                                           >  2:590014/1‑140 (MQ=255)
                                                                                            aGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTa                                           >  2:424105/1‑140 (MQ=255)
                                                                                            aGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTa                                           >  2:21463/1‑140 (MQ=255)
                                                                                                 agaaCGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAAtttt                                      >  2:495238/1‑139 (MQ=255)
                                                                                                 agaaCGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTg                                      >  2:742897/1‑140 (MQ=255)
                                                                                                 agaaCGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTg                                      >  1:324553/1‑140 (MQ=255)
                                                                                                 agaaCGCGGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTg                                      >  2:1149565/1‑140 (MQ=255)
                                                                                                     cgcgGGATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCa                                  <  2:707101/140‑1 (MQ=255)
                                                                                                          gATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCAt                             <  2:1152035/140‑1 (MQ=255)
                                                                                                          gATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCAt                             <  2:757027/140‑1 (MQ=255)
                                                                                                          gATGTTCATTCTTCATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCAt                             <  2:1104230/140‑1 (MQ=255)
                                                                                                                    cttcATCACTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGa                   >  1:387953/1‑140 (MQ=255)
                                                                                                                        atcaCTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGc               >  1:328939/1‑140 (MQ=255)
                                                                                                                        atcaCTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGc               >  2:527723/1‑140 (MQ=255)
                                                                                                                         tcaCTTTTAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCg              >  2:1024645/1‑140 (MQ=255)
                                                                                                                               ttAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGtt        >  1:18450/1‑140 (MQ=255)
                                                                                                                               ttAATTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGtt        >  2:1262772/1‑140 (MQ=255)
                                                                                                                                  aTTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTgtg     <  1:21463/140‑3 (MQ=255)
                                                                                                                                  aTTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCg     <  1:590014/140‑1 (MQ=255)
                                                                                                                                  aTTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCg     <  2:543124/140‑1 (MQ=255)
                                                                                                                                  aTTTATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCg     <  2:18450/140‑1 (MQ=255)
                                                                                                                                     gATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGgt  <  1:527723/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
CAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGT  >  NC_000913/580140‑580412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A