Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F48 I1 R1	45	83.3	2696702	99.2%	2675128	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,758	G→T	S413S (TCG→TCT)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,758	0	G	T	100.0%	156.2 / NA	44	S413S (TCG→TCT)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (21/23); total (21/23)

CGTTGCTGCGCCTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCGCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGC  >  NC_000913/868625‑868889

cgTTGCTGCGCCTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGAc                                                                                                                               <  2:1102460/140‑1 (MQ=255)

      tgcGCCTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGt                                                                                                                         <  2:944567/140‑1 (MQ=255)

         gcCTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAg                                                                                                                      >  1:486644/1‑140 (MQ=255)

          ccTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGa                                                                                                                     >  2:997519/1‑140 (MQ=255)

            tGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGAcc                                                                                                                   >  2:265111/1‑140 (MQ=255)

             ggTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCa                                                                                                                  >  2:98575/1‑140 (MQ=255)

             ggTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCa                                                                                                                  >  1:536226/1‑140 (MQ=255)

              gTCGAATCGCAGGGCGGCGAAAATATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCAt                                                                                                                 <  1:98575/140‑1 (MQ=255)

               tCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATc                                                                                                                >  2:1244389/1‑140 (MQ=255)

                  aaTCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGt                                                                                                             >  2:541634/1‑140 (MQ=255)

                    tCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTgt                                                                                                           >  2:582075/1‑139 (MQ=255)

                       cAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTc                                                                                                        <  1:752700/140‑1 (MQ=255)

                            cggcgAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTa                                                                                                   <  2:248016/140‑1 (MQ=255)

                            cggcgAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTa                                                                                                   <  2:720602/140‑1 (MQ=255)

                                 aaaTTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGaa                                                                                              <  2:486644/140‑1 (MQ=255)

                                   aTTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAAcc                                                                                            <  1:1244389/140‑1 (MQ=255)

                                       tCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTg                                                                                        <  1:541634/140‑1 (MQ=255)

                                       acTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCAGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTg                                                                                        <  2:336007/139‑1 (MQ=255)

                                        cTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGc                                                                                       <  1:265111/140‑1 (MQ=255)

                                        cTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGc                                                                                       <  1:1099230/140‑1 (MQ=255)

                                            aaCGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCgtgt                                                                                   >  2:858365/1‑140 (MQ=255)

                                               ggTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTaca                                                                                <  2:810743/140‑1 (MQ=255)

                                                   aGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGt                                                                            >  2:933262/1‑140 (MQ=255)

                                                        aTCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTAtt                                                                       >  1:1120035/1‑140 (MQ=255)

                                                                 ttGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTaa                                                              >  1:287599/1‑140 (MQ=255)

                                                                  tGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTaaa                                                             >  2:1276015/1‑140 (MQ=255)

                                                                  tGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTaaa                                                             >  2:60889/1‑140 (MQ=255)

                                                                   gTCACCCGGCAAACTCCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAg                                                            >  2:477395/1‑140 (MQ=255)

                                                                      aaccGGCAAACTTCAGGCATTAAGCCAGAATATTGAGTCTATTTTACAGGCACCTTACGCTTATAAGGACTCAAGTAAGACCATCGGTGATTCGATTATGGCAAAGCTGAGTGTACACGGTTTATTGCCAGGTAAAGAcg                                                         <  2:1268696/138‑1 (MQ=255)

                                                                          ggCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGTGGATTCGATTATCGAACCGCTGCGTGTACACTGTTTATTGCCAGGTAAAGACGCGGc                                                     >  1:1268696/1‑140 (MQ=255)

                                                                           gCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCt                                                    >  2:762942/1‑140 (MQ=255)

                                                                               aCTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCAc                                                <  1:858365/140‑1 (MQ=255)

                                                                                cTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCAcg                                               <  2:324056/140‑1 (MQ=255)

                                                                                  tCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCAcgcg                                             <  2:1120035/140‑1 (MQ=255)

                                                                                        aatACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGt                                       <  1:1238923/138‑1 (MQ=255)

                                                                                        aTTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGt                                       <  1:997519/140‑1 (MQ=255)

                                                                                          tACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTgg                                     >  2:988309/1‑140 (MQ=255)

                                                                                           aCGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGt                                    >  1:14228/1‑140 (MQ=255)

                                                                                           aCGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGt                                    >  2:955007/1‑140 (MQ=255)

                                                                                                       tCAGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGcgcg                        <  2:536226/140‑1 (MQ=255)

                                                                                                         aGTTTATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTg                      <  1:1276015/140‑1 (MQ=255)

                                                                                                           tttATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTggg                    >  2:351675/1‑140 (MQ=255)

                                                                                                            ttATTTTTCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGc                   >  1:986307/1‑140 (MQ=255)

                                                                                                                  ttCAGGACCCTTACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTa             <  2:14228/140‑1 (MQ=255)

                                                                                                                            ttACGCTTCTCTGAACCCACGTCAGACCATCGGTGATTTGATTATCGAACCGCCGCGCGGACACGGTTTCATCCCAGGTAAAGACGCGGCTGCTCGCGCTGCGTGGCTGCTGGAGCGCGTGGGCCTGCTTCCTGAACAtt   >  2:741112/1‑139 (MQ=255)

                                                                                                                             tACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGc  <  1:762942/140‑1 (MQ=255)

                                                                                                                             tACGCTTCTCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGc  <  2:986307/140‑1 (MQ=255)

CGTTGCTGCGCCTGGTCGAATCGCAGGGCGGCGAAATTATCTTTAACGGTCAGCGAATCGATACCTTGTCACCCGGCAAACTTCAGGCATTACGCCGGGATATTCAGTTTATTTTTCAGGACCCTTACGCTTCGCTGGACCCACGTCAGACCATCGGTGATTCGATTATCGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGC  >  NC_000913/868625‑868889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A