Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F48 I1 R1 55 131.7 4456318 99.2% 4420667 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 127,167 G→T K491N (AAG→AAT)  aceF → pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913127,1670GT100.0% 366.2 / NA 103K491N (AAG→AAT) aceFpyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/59);  total (44/59)

Only 100 of 104 total aligned reads displayed.

AGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGA  >  NC_000913/127032‑127298
                                                                                                                                       |                                                                                                                                   
agaagCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAAt                                                                                                                                 >  1:1868296/1‑140 (MQ=255)
agaagCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAAt                                                                                                                                 >  2:1172382/1‑140 (MQ=255)
  aagCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATAc                                                                                                                               <  1:1101684/140‑1 (MQ=255)
  aagCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATAc                                                                                                                               <  2:2208723/140‑1 (MQ=255)
     cggcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATc                                                                                                                            >  1:1435891/1‑140 (MQ=255)
     cggcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATc                                                                                                                            >  1:1841015/1‑140 (MQ=255)
      ggcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCa                                                                                                                           >  2:252723/1‑140 (MQ=255)
       gcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCaa                                                                                                                          >  2:2166946/1‑140 (MQ=255)
        cgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAAc                                                                                                                         <  2:1375540/140‑1 (MQ=255)
          aaaCGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACAt                                                                                                                       <  2:243497/140‑1 (MQ=255)
               tAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGgtg                                                                                                                  <  2:901286/140‑1 (MQ=255)
               tAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGgtg                                                                                                                  <  2:771993/140‑1 (MQ=255)
               tAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGgtg                                                                                                                  <  2:1868296/140‑1 (MQ=255)
                 aGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGtgtg                                                                                                                <  1:484309/140‑1 (MQ=255)
                   cTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGc                                                                                                              <  1:252723/140‑1 (MQ=255)
                   cTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGc                                                                                                              <  1:1748834/140‑1 (MQ=255)
                   cTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGc                                                                                                              <  1:1104056/140‑1 (MQ=255)
                      gATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGt                                                                                                           <  2:1401799/140‑1 (MQ=255)
                       aTGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTg                                                                                                          >  1:381683/1‑140 (MQ=255)
                              gATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGACGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCTGTGTGGCGGCGGGTAccc                                                                                                   >  2:1174931/1‑140 (MQ=255)
                                tCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCg                                                                                                 >  2:932835/1‑140 (MQ=255)
                                tCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCg                                                                                                 <  2:1382694/140‑1 (MQ=255)
                                tCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCg                                                                                                 <  2:2105638/140‑1 (MQ=255)
                                 cACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGGTACCCCgc                                                                                                >  2:210051/1‑139 (MQ=255)
                                 cACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGa                                                                                                >  2:175489/1‑140 (MQ=255)
                                 cACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGa                                                                                                >  2:1981924/1‑140 (MQ=255)
                                 cACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGATCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGa                                                                                                <  1:1546418/140‑1 (MQ=255)
                                  cccccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               <  1:1172569/139‑1 (MQ=255)
                                  aCCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCTaa                                                                                               <  2:1435891/140‑1 (MQ=255)
                                  aCCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               <  1:1440454/140‑1 (MQ=255)
                                  aCCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               <  2:1588192/140‑1 (MQ=255)
                                  aCCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               <  1:1368058/140‑1 (MQ=255)
                                  aCCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               >  1:1337095/1‑140 (MQ=255)
                                   ccccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAAc                                                                                              >  2:456423/1‑140 (MQ=255)
                                    ccctgTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACg                                                                                             >  1:1959521/5‑140 (MQ=255)
                                    cccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACGCT‑AATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACgg                                                                                            <  1:384447/140‑1 (MQ=255)
                                    cccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGTTACCCCCAACg                                                                                             >  1:762912/1‑140 (MQ=255)
                                     ccGGTTGTCTTCATCATGAAAGCCGTTGCAGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACgg                                                                                            <  2:534552/140‑1 (MQ=255)
                                      cGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACggg                                                                                           >  1:2023658/1‑139 (MQ=255)
                                      cGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGt                                                                                           <  1:1172382/140‑1 (MQ=255)
                                         ttGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               <  2:600298/133‑1 (MQ=255)
                                         ttGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGaa                                                                                               >  1:600298/1‑133 (MQ=255)
                                         ttGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTg                                                                                        >  1:385782/1‑140 (MQ=255)
                                           gTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGgt                                                                                      >  1:1856641/1‑140 (MQ=255)
                                            tCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGgtt                                                                                     >  1:1144866/1‑140 (MQ=255)
                                             cTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGgttg                                                                                    <  2:129839/140‑1 (MQ=255)
                                                catcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTc                                                                                 <  1:1847485/140‑1 (MQ=255)
                                                catcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTc                                                                                 <  1:1981924/140‑1 (MQ=255)
                                                 atcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTcc                                                                                >  1:278568/1‑140 (MQ=255)
                                                 atcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTAGCGGTGGATACCCCGAACGGTCTGGTTGTTcc                                                                                >  2:301812/1‑140 (MQ=255)
                                                  tcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATGCCCCGAACGGTCTGGTTGTTCCg                                                                               <  1:1686427/140‑1 (MQ=255)
                                                  tcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGCACGGTCTGGTTGTTCCg                                                                               <  1:1618120/140‑1 (MQ=255)
                                                  tcatGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCg                                                                               <  2:381683/140‑1 (MQ=255)
                                                   catGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGCGGATACCCCCCACGGGCTGGTTGTTCCgt                                                                              >  2:1686427/1‑139 (MQ=255)
                                                         aGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCa                                                                        <  1:932835/140‑1 (MQ=255)
                                                           ccGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCaaa                                                                      <  2:385782/140‑1 (MQ=255)
                                                            cGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAg                                                                     <  2:362376/140‑1 (MQ=255)
                                                            cGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTAGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAg                                                                     <  1:301812/140‑1 (MQ=255)
                                                               tgctgcAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACg                                                                  >  2:2010028/1‑140 (MQ=255)
                                                               tgctgcAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACg                                                                  <  1:1174931/140‑1 (MQ=255)
                                                                gctgcAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGCACGGTCTGGTTGTTCCGGTATTCAAAGACGt                                                                 >  2:1626266/1‑140 (MQ=255)
                                                                   gcAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTcaa                                                              <  1:1478492/140‑1 (MQ=255)
                                                                    cAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTcaac                                                             <  1:456423/140‑1 (MQ=255)
                                                                         ctTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGaa                                                        >  1:5054/1‑140 (MQ=255)
                                                                                 gATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGcatca                                                <  1:2008172/140‑1 (MQ=255)
                                                                                  aTGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGATGTCAACAAGAAAGGcatcat                                               >  2:1499658/1‑140 (MQ=255)
                                                                                    gCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCg                                             >  1:1554682/1‑140 (MQ=255)
                                                                                    gCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCg                                             >  1:1128333/1‑140 (MQ=255)
                                                                                      cTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAg                                           <  2:1841015/140‑1 (MQ=255)
                                                                                       tCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGCGGATACCCCCCAAAGTCCGGTTTTTTCCGGATTCCAAAACACCAACAAGAAAAGcatcaacccac                                          >  2:1580784/1‑134 (MQ=255)
                                                                                        cGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCt                                         <  2:1144866/140‑1 (MQ=255)
                                                                                              aaTAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTcg                                   <  1:973336/140‑1 (MQ=255)
                                                                                                tAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTcgcg                                 <  1:970917/140‑1 (MQ=255)
                                                                                                  gTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAg                               <  1:1626266/140‑1 (MQ=255)
                                                                                                   ttCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTTCGGGATTCAAAGACGTCAACAAGAAAAGCATCATCGAGCTGTCTCGCGAGc                              >  2:70481/1‑140 (MQ=255)
                                                                                                   ttCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCCAACGGGCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCgcgcgc                              >  2:406906/1‑140 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGGTATCCCCGACGGTCTGGTTGTTCCCGTTTTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTTTCGCGAGCtt                            >  2:882590/1‑139 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAg                               >  1:658226/1‑137 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAg                               <  2:658226/137‑1 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtg                            >  1:1350120/1‑140 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtg                            <  2:1337095/140‑1 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAAAAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtg                            >  2:1210468/1‑140 (MQ=255)
                                                                                                     cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGGCTGGTTGTTTCGGGATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtg                            >  1:567998/1‑140 (MQ=255)
                                                                                                       cTGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGGCTGGTTGTTTCGGTATTTAAAGACGTCAACAAGAAAAGCATCATCGAGCTGTTTCGCGAGCtgat                          >  2:2121879/1‑140 (MQ=255)
                                                                                                        tGTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtgatg                         >  1:569651/1‑140 (MQ=255)
                                                                                                         gTCGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtgatga                        <  2:5054/140‑1 (MQ=255)
                                                                                                           cGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACt                      <  1:2010028/140‑1 (MQ=255)
                                                                                                           cGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACt                      <  1:936935/140‑1 (MQ=255)
                                                                                                           cGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACt                      <  2:1554682/140‑1 (MQ=255)
                                                                                                           cGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACt                      <  2:1128333/140‑1 (MQ=255)
                                                                                                           cGGAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACt                      <  1:958389/140‑1 (MQ=255)
                                                                                                             gAAGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTAt                    <  2:1328678/140‑1 (MQ=255)
                                                                                                               aGACGGTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTAttt                  <  1:1092978/140‑1 (MQ=255)
                                                                                                                    gTCAGCGTCTGACCCTGAATAAATACTTCAACATCGGTGTGGCGGTGGTTACCCCGAACGGTCTGGTTGTTTCCGTATTTTAAGACGTCAACAAGAAAAGCAACATCGAGCTTTTTCGCGAGCTGATTACTAttttttaa             >  2:1564396/1‑135 (MQ=255)
                                                                                                                    gTCAGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGGTACCCCGAACGGGCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTTTCTCGCGAGCTGATGACTATTTTTaaa             >  2:189945/1‑139 (MQ=255)
                                                                                                                       aGCGTCTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGaaa          <  1:175489/140‑1 (MQ=255)
                                                                                                                            ttGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGcgcg     <  2:762912/139‑1 (MQ=255)
                                                                                                                            cTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGcgcg     <  2:1350120/140‑1 (MQ=255)
                                                                                                                            cTGACCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGcgcg     <  2:1856641/140‑1 (MQ=255)
                                                                                                                               aCCCTGAATAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGa  >  1:254468/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGA  >  NC_000913/127032‑127298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A