Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F48 I1 R1
|
61 |
74.3 |
2613450 |
99.1% |
2589928 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
447,009 |
A→T |
Y233N (TAT→AAT) |
cyoE ← |
heme O synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 447,009 | 0 | A | T | 98.3%
| 203.6
/ ‑3.7
| 59 | Y233N (TAT→AAT) | cyoE | heme O synthase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (33/25); total (34/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 |
CACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGT > NC_000913/446875‑447143
|
caCCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTtat > 2:888228/1‑140 (MQ=255)
caCCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTtat > 1:834150/1‑140 (MQ=255)
aCCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTtata < 1:391491/140‑1 (MQ=255)
agagGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCa > 1:675492/1‑140 (MQ=255)
ggCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGt > 2:736964/1‑140 (MQ=255)
gCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTa > 2:502381/1‑140 (MQ=255)
aGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAAc > 2:266206/1‑140 (MQ=255)
aTGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGcc > 2:1190458/1‑140 (MQ=255)
ggataaaagagAAGCCAAACAGTTGGCGCCCCAAGTTTCTGTAATAAGAAACTTAAAAC‑CCGCGCGGACCAAT‑CCCAACCCCCAAG‑ACCCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAaga < 2:566873/133‑1 (MQ=255)
cGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAaga > 2:573445/1‑140 (MQ=255)
agaatAGAGAACCAAAACAGCTTGGGCCCCAAAATTTGGTCATCGACAACTTTAAAA‑CCGCGCAGACCCAT‑CCCTACCCCCCAG‑ACCATACCCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAagaga < 2:772353/137‑1 (MQ=255)
gatAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAagagaga > 2:133835/1‑140 (MQ=255)
agaAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATc > 1:13499/1‑140 (MQ=255)
agaAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATc > 2:820573/1‑140 (MQ=255)
aGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGc > 1:223945/1‑140 (MQ=255)
gCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCg > 2:861942/1‑140 (MQ=255)
ccGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGt > 1:1281725/1‑140 (MQ=255)
ccGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGt > 1:457558/1‑140 (MQ=255)
cGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTg > 1:990211/1‑140 (MQ=255)
cGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTg > 1:902765/1‑140 (MQ=255)
ggcccccAGATTCGCGGATCAG‑AACTTTAAAACCCACGCAGCGCCA‑‑ACCCAACCACCAGAACAATAACCGCCGCGGCGACCACCAGATTTTTATACCCCGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCaaa < 2:221154/136‑1 (MQ=255)
cgcCAAAATTCTTCCATCAGCAACTTTAAAC‑CCGCCAAGAGCCAT‑ACCAACCCACCAG‑ACGCAAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAgg < 2:5384/140‑1 (MQ=255)
agcaaaGATTCTGTAACAAGAATTTTTAAAA‑CCGC‑CAAAACCATCACCAACCCACCAG‑ACGCTACCCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAgg < 2:287438/135‑1 (MQ=255)
agcCAAGATTCTGTCATCAGCAACTTAATAC‑CCGCGAAGACCCAT‑CCCAACCCACAAG‑ACGCTACCCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAgg < 2:29121/139‑1 (MQ=255)
ccaaGATTCTGTCATCAGCAACTTTATAC‑CCGCGCAGAGCAAT‑ACCTACCCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCg < 2:675492/137‑1 (MQ=255)
aGATTCTGTAATCAGAAATTTTAAAA‑CCGCGCAGAGCCAT‑ACCAACCCACCAG‑ACGCAAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCgatg < 2:186617/140‑1 (MQ=255)
tcatcaGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCTGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAg > 1:38472/1‑140 (MQ=255)
gcatcaGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAg > 2:806908/2‑140 (MQ=255)
atcaGCAATTTTAAAA‑CCCCCCAGAGCCAT‑ACCAAACCCCCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCg < 2:1236652/140‑1 (MQ=255)
accaacAACTTTCAAC‑CCGCGCAGAGACAT‑CCCTAACCCCAAG‑CCCCCAACCGCCGCGGCGACCACCAGATATTTAACCCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCg < 2:457558/135‑1 (MQ=255)
taA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGAttctt > 1:579222/1‑140 (MQ=255)
cgcAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCAc > 2:229205/1‑140 (MQ=255)
cgcAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCAc > 1:305587/1‑140 (MQ=255)
acccAT‑ACCAACCCACCAG‑ACGATACCCGCCGCGGCGACCACAAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGaaa < 2:305587/138‑1 (MQ=255)
t‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCt > 2:507872/1‑140 (MQ=255)
tAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTa < 1:229205/140‑1 (MQ=255)
accaccAG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTAcc > 2:806242/1‑140 (MQ=255)
accacaAG‑CCGCAACCCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTAcc < 2:13499/140‑1 (MQ=255)
cccccaG‑ACGATAACCGCCGCGGCGACCCCCAGATTTTTATACAAAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCa < 2:820387/137‑1 (MQ=255)
ccaccaG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCa > 2:1241523/1‑140 (MQ=255)
ccaccaG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCa > 2:281007/1‑140 (MQ=255)
caG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTgg > 1:995074/1‑140 (MQ=255)
caG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTgg > 1:511008/1‑140 (MQ=255)
caG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTgg > 1:620671/1‑140 (MQ=255)
aG‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGc > 2:257496/1‑140 (MQ=255)
g‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCa > 1:1296590/1‑140 (MQ=255)
g‑ACGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCa < 1:728563/140‑1 (MQ=255)
aCGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCaa > 2:1001600/1‑140 (MQ=255)
aCGCTAACCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCaa < 1:133835/140‑1 (MQ=255)
aaaaCCGCCGCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATAc < 2:355668/138‑1 (MQ=255)
ccgccgCGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGa < 1:257496/140‑1 (MQ=255)
gccgcGGCGACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAAt > 1:992831/1‑140 (MQ=255)
cgcgGCGACCACCAGATTTTTATACCCAGCGAAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAAAGt > 1:450729/1‑140 (MQ=255)
ggcgACCGCCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTg < 1:1138271/140‑1 (MQ=255)
ggcgACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTg < 1:1001600/140‑1 (MQ=255)
cgACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTgcc < 1:1190458/140‑1 (MQ=255)
gACCACCAGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTgccg < 1:575565/140‑1 (MQ=255)
caGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGt < 1:1116883/140‑1 (MQ=255)
caGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGt < 1:736964/140‑1 (MQ=255)
caGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGt < 1:861942/140‑1 (MQ=255)
aaGATTTTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGt < 2:620671/139‑1 (MQ=255)
|
CACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAA‑CCGCGCAGAGCCAT‑ACCTAACCACCAG‑ACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGT > NC_000913/446875‑447143
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
