Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F48 I1 R1	61	74.3	2613450	99.1%	2589928	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	598,114	G→T	A212A (GCG→GCT)	cusB →	copper/silver export system membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	598,114	0	G	T	100.0%	131.5 / NA	39	A212A (GCG→GCT)	cusB	copper/silver export system membrane fusion protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (6/33); total (6/33)

CTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCGCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGG  >  NC_000913/597977‑598246

ctgctgCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTcc                                                                                                                                    >  2:1245108/1‑140 (MQ=255)

ctgctgCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTcc                                                                                                                                    >  1:1111954/1‑140 (MQ=255)

  gctgcGCGAAACCGGCGGTACGGCGACCCCGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGGACGTCCGCGGCCGACATTTGCCGCCCGTTTGCCCCACAAAAAATTTACACTCC‑CTTTTCGCACACAACGccct                                                                                                                                  >  2:1056996/1‑139 (MQ=255)

  gctgcGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCa                                                                                                                                  >  1:1042530/1‑140 (MQ=255)

  gctgcGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAAAGCCCGGGGCGGGTATTTGCCGCCCGATCGCCCCGCCAAAAATCCAGACTCGCCTTTAC‑CTCCAAGCTcccc                                                                                                                                  >  2:1095543/1‑139 (MQ=255)

     gcgcGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTg                                                                                                                               <  2:164696/140‑1 (MQ=255)

      cgcgAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGGGGCGGATATTCGCCGCCCGATCGCCCCGCAAAAAAACCAGAATCG‑CTTTACGCTCAAAGCTCCCCTTGa                                                                                                                              >  2:878366/1‑140 (MQ=255)

                  ggTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCAc                                                                                                                  <  1:547736/140‑1 (MQ=255)

                  ggTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCAc                                                                                                                  <  2:1111954/140‑1 (MQ=255)

                  ggTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACACAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCAc                                                                                                                  <  2:331083/140‑1 (MQ=255)

                  ggTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGAGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCAc                                                                                                                  <  2:1292238/140‑1 (MQ=255)

                           aCCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGGTATTCGCCGCCCGGTCGCCACGCAAAAAAACCCGACTCG‑CGTTACGCTCAAAGCTCCCCTTGATGGCGTGATCACCGCGTTTgt                                                                                                         >  2:1062247/1‑139 (MQ=255)

                              cAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCt                                                                                                      <  2:251606/140‑1 (MQ=255)

                                 aCTGAAGGCATTCTCGAGCGGCTGCGACTGGTGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcg                                                                                                   >  2:1254552/1‑140 (MQ=255)

                                   tGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAACGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcg                                                                                                 <  1:915746/140‑1 (MQ=255)

                                   tGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcg                                                                                                 <  1:1245108/140‑1 (MQ=255)

                                   tGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcg                                                                                                 <  2:566247/140‑1 (MQ=255)

                                   tGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcg                                                                                                 <  2:528388/140‑1 (MQ=255)

                                   tGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcg                                                                                                 <  1:138469/140‑1 (MQ=255)

                                    gAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcgc                                                                                                <  2:493543/140‑1 (MQ=255)

                                    gAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTgcgcgc                                                                                                <  2:490974/140‑1 (MQ=255)

                                     aaGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGcgcgcg                                                                                               <  2:101723/140‑1 (MQ=255)

                                      aGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGGGGAGATTTCCCGCCTGATCGCCACGCCAAAAAAACCGACACC‑CTTTACGCCCAAAGCTCCCCTTTATGGCGTGATCACCGCGTTTGATCTTCGCGCgg                                                                                              >  2:103459/1‑140 (MQ=255)

                                       ggCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCggg                                                                                             <  1:878366/140‑1 (MQ=255)

                                        gCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGga                                                                                            <  1:395058/140‑1 (MQ=255)

                                               tGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAata                                                                                     <  1:761601/140‑1 (MQ=255)

                                               tGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAata                                                                                     <  1:1056996/140‑1 (MQ=255)

                                                  gCGGCTGCGACTGGCGGGAATGCCGGAGGCGGGTATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCg                                                                                  >  2:625108/1‑140 (MQ=255)

                                                    ggCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGcc                                                                                <  1:190310/140‑1 (MQ=255)

                                                       tGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCaaa                                                                             <  1:576506/140‑1 (MQ=255)

                                                         cGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGa                                                                           <  1:708760/140‑1 (MQ=255)

                                                                        ccGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGaa                                                            <  2:361318/140‑1 (MQ=255)

                                                                            aGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAAtt                                                        <  1:1095543/140‑1 (MQ=255)

                                                                              gCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCGTTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCa                                                      <  2:505540/140‑1 (MQ=255)

                                                                              gCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCa                                                      <  2:108937/140‑1 (MQ=255)

                                                                              gCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCa                                                      <  1:147860/140‑1 (MQ=255)

                                                                              gCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCa                                                      <  1:76116/140‑1 (MQ=255)

                                                                               cGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAg                                                     <  1:103459/140‑1 (MQ=255)

                                                                                 gATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAggg                                                   >  1:617944/1‑140 (MQ=255)

                                                                                         ccgccTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGAcc                                           <  1:625108/140‑1 (MQ=255)

                                                                                         ccgccTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGAcc                                           <  2:1042530/140‑1 (MQ=255)

                                                                                           gccTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCg                                         >  2:592131/1‑140 (MQ=255)

                                                                                                                        cG‑CTTTACGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTc            >  1:151451/1‑140 (MQ=255)

                                                                                                                                cGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGcc     <  2:1199017/140‑1 (MQ=255)

                                                                                                                                cGCTCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGcc     >  2:778238/1‑140 (MQ=255)

                                                                                                                                   tCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCctgg  <  2:151451/140‑1 (MQ=255)

                                                                                                                                   tCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCctgg  <  2:365929/140‑1 (MQ=255)

                                                                                                                                   tCAAAGCTCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCctgg  <  1:797518/140‑1 (MQ=255)

CTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGCATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCCACGCAAAAAATCCAGACTCG‑CTTTACGCTCAAAGCGCCCATTGATGGCGTGATCACCGCGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGG  >  NC_000913/597977‑598246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A