Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F48 I1 R1	62	93.4	3176452	99.2%	3151040	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	162,800	G→T	L232L (CTG→CTT)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	162,800	0	G	T	100.0%	279.6 / NA	78	L232L (CTG→CTT)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (43/35); total (43/35)

CTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCA  >  NC_000913/162672‑162936

cTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCAt                                                                                                                               <  2:1254887/140‑1 (MQ=255)

cTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCAt                                                                                                                               >  2:429796/1‑140 (MQ=255)

   cccGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCgg                                                                                                                            <  1:952295/140‑1 (MQ=255)

   cccGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCgg                                                                                                                            >  2:256324/1‑140 (MQ=255)

      gcgcATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAg                                                                                                                         <  1:822963/140‑1 (MQ=255)

      gcgcATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAg                                                                                                                         <  2:1089337/140‑1 (MQ=255)

        gcATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTg                                                                                                                       <  1:1405804/140‑1 (MQ=255)

        gcATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTg                                                                                                                       <  1:1321734/140‑1 (MQ=255)

        gcATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTg                                                                                                                       <  1:1384691/140‑1 (MQ=255)

             aGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGtt                                                                                                                  >  2:536028/1‑139 (MQ=255)

                  tttGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGct                                                                                                             <  2:1224733/140‑1 (MQ=255)

                    tGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGctct                                                                                                           <  1:536028/140‑1 (MQ=255)

                    tGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGctct                                                                                                           >  1:1505563/1‑140 (MQ=255)

                    tGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGctct                                                                                                           >  2:1446618/1‑140 (MQ=255)

                      aCGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGc                                                                                                         >  2:630517/1‑140 (MQ=255)

                      aCGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGc                                                                                                         <  2:1055034/140‑1 (MQ=255)

                         aTGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCg                                                                                                      >  1:910219/1‑140 (MQ=255)

                         aTGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCg                                                                                                      >  2:1009102/1‑140 (MQ=255)

                                        ccACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTg                                                                                       <  1:870797/140‑1 (MQ=255)

                                         cACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGt                                                                                      >  2:193511/1‑140 (MQ=255)

                                          aCCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTc                                                                                     <  1:1167013/140‑1 (MQ=255)

                                            cGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGc                                                                                   >  1:955819/1‑140 (MQ=255)

                                                 aaaTGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAAc                                                                              >  2:1420565/1‑140 (MQ=255)

                                                 aaaTGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAAc                                                                              >  1:1073600/1‑140 (MQ=255)

                                                     gctgcGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATc                                                                          <  1:81881/140‑1 (MQ=255)

                                                      ctgcGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCa                                                                         >  1:1084123/1‑140 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      >  2:675301/1‑140 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      <  2:910219/140‑1 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      <  2:955819/140‑1 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      >  2:979734/1‑140 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      >  1:1338740/1‑140 (MQ=255)

                                                         cGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCg                                                                      >  1:230278/1‑140 (MQ=255)

                                                           tCAGGAATGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGCCGGGGATCGATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCatggaa                                                                    >  2:334074/1‑135 (MQ=255)

                                                           tCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGaa                                                                    <  1:112609/140‑1 (MQ=255)

                                                                aaaGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCg                                                               <  1:351628/140‑1 (MQ=255)

                                                                   gCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGAtc                                                            >  2:1141005/1‑140 (MQ=255)

                                                                      gATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCTCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGAtcctc                                                         >  2:1334969/1‑140 (MQ=255)

                                                                       aTCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGAtcctcc                                                        <  2:1338740/140‑1 (MQ=255)

                                                                         cATTACTGTTATTTTTACCTGGCGTCGGCGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCCCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCg                                                      >  2:855256/1‑140 (MQ=255)

                                                                          aTTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCgg                                                     <  1:1420565/140‑1 (MQ=255)

                                                                          aTTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCgg                                                     <  1:429796/140‑1 (MQ=255)

                                                                           ttACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTCTTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTCAACGCTCAGCGAAAAGCGATCCTCCCgac                                                    <  2:1153963/140‑3 (MQ=255)

                                                                              cTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCAcc                                                 >  1:797005/1‑140 (MQ=255)

                                                                              cTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCAcc                                                 >  1:1132297/1‑140 (MQ=255)

                                                                              cTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCAcc                                                 >  1:1073758/1‑140 (MQ=255)

                                                                                 ttATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCa                                              >  2:621514/1‑140 (MQ=255)

                                                                                 ttATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCa                                              >  1:601532/1‑140 (MQ=255)

                                                                                   aTTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAg                                            >  1:206728/1‑140 (MQ=255)

                                                                                   aTTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAg                                            >  1:470804/1‑140 (MQ=255)

                                                                                   aTTTTTACCTGGCGTAGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAg                                            >  1:1035479/1‑140 (MQ=255)

                                                                                     ttttACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAGCGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAggg                                          <  1:256324/140‑1 (MQ=255)

                                                                                      tttACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGa                                         >  2:297079/1‑140 (MQ=255)

                                                                                       ttACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGAt                                        <  2:230278/140‑1 (MQ=255)

                                                                                              gCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCTTCGGCAGTGATTTATTGCTCTGCCCGCTGTATTGCGCGTTGGCGCTGAACGATCAGCGAAAATCGATCCTCCCTGCACCGAAAGGGACGCGCaaa                                 >  2:607805/1‑140 (MQ=255)

                                                                                              gCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCaaa                                 >  2:753836/1‑140 (MQ=255)

                                                                                              gCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCaaa                                 >  2:740053/1‑140 (MQ=255)

                                                                                              gCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCaaa                                 >  1:1352170/1‑140 (MQ=255)

                                                                                                 tCGGAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAgtg                              >  2:463615/1‑140 (MQ=255)

                                                                                                   ggAGAAATTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAgtggt                            >  2:878114/1‑140 (MQ=255)

                                                                                                       aaaTTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTg                        <  1:580471/140‑1 (MQ=255)

                                                                                                       aaaTTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTg                        <  2:470804/140‑1 (MQ=255)

                                                                                                        aaTTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTgg                       <  2:1352170/140‑1 (MQ=255)

                                                                                                         aTTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGc                      >  2:869351/1‑140 (MQ=255)

                                                                                                         aTTCAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGc                      >  1:1178902/1‑140 (MQ=255)

                                                                                                            cAGCGTGTGCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGAc                   <  1:1136913/140‑1 (MQ=255)

                                                                                                                 tgtgCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAata              >  2:549663/1‑140 (MQ=255)

                                                                                                                 tgtgCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAata              >  2:789484/1‑140 (MQ=255)

                                                                                                                   tgCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATAtt            >  2:1466686/1‑140 (MQ=255)

                                                                                                                    gCAGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTg           >  1:1072033/1‑140 (MQ=255)

                                                                                                                      aGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCt         <  1:1009102/140‑1 (MQ=255)

                                                                                                                      aGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCt         <  1:863249/140‑1 (MQ=255)

                                                                                                                      aGGAACAACTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCt         <  1:753836/140‑1 (MQ=255)

                                                                                                                         aacaacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaa      <  2:1132297/140‑1 (MQ=255)

                                                                                                                          acaacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa     <  2:819682/140‑1 (MQ=255)

                                                                                                                          acaacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa     <  2:645174/140‑1 (MQ=255)

                                                                                                                          acaacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa     <  2:601532/140‑1 (MQ=255)

                                                                                                                          acaacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa     <  1:812176/140‑1 (MQ=255)

                                                                                                                           caacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAAc    >  1:608566/1‑140 (MQ=255)

                                                                                                                           caacTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAAc    <  2:1505563/140‑1 (MQ=255)

                                                                                                                             acTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa  >  2:947667/1‑140 (MQ=255)

                                                                                                                             acTTGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa  >  2:1509647/1‑140 (MQ=255)

CTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCA  >  NC_000913/162672‑162936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A