Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F56 I15 R1	126	117.1	3948514	95.2%	3758985	126.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,031	C→T	A46A (GCG→GCA)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,031	0	C	T	100.0%	112.9 / NA	36	A46A (GCG→GCA)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/30); total (6/30)

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAA  >  CP006881/3724896‑3725160

cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGGTTTGTGGTTTTGTTGCTCATTAACCCCCCGCCGCCCATCATAAAAAGAAACCAGTCCagcc                                                                                                                                >  7:454951/1‑138 (MQ=255)

cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                                >  8:375701/1‑139 (MQ=255)

cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGCGTCCtgcg                                                                                                                                >  1:36113/1‑139 (MQ=255)

  ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                              >  7:992/1‑139 (MQ=255)

  ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTTGTGGGTTTGTTCCGCATTCACAGCCCTCCGCCATTAATAAAACGAAACGAGTCCTGCGGc                                                                                                                              >  7:109543/1‑139 (MQ=255)

               cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                 <  5:223803/139‑2 (MQ=255)

                  cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctt                                                                                                               <  1:210621/138‑1 (MQ=255)

                  cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTTCATTTGTTTCTCATTCACAGCCCTCCCCCATTGATAAAACGAAACCAGTACTGCCGCCTGCACcgccgccaat                                                                                                              >  2:56740/1‑135 (MQ=255)

                      ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTGCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                           <  3:246301/138‑1 (MQ=255)

                       tcGATTCCTTGAACGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGGATTATGCTTTTCATCCTCATTCACAGCCATCCGCCATTCAAAAATCGAAAGGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  1:27840/137‑1 (MQ=255)

                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTATGGTTTTGTTACTCCTTAACAGCCCTACGCCATTAATAAAACCAAACGAGTCCTGCGGCCGGCCCCGCCGCCGTTCtttt                                                                                                         >  8:219721/1‑138 (MQ=255)

                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  2:79851/138‑1 (MQ=255)

                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  8:238747/138‑1 (MQ=255)

                            tCCTTGATGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                     <  1:183372/138‑1 (MQ=255)

                                 gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                <  7:3706/138‑1 (MQ=255)

                                  aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCGTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                              <  3:214660/139‑1 (MQ=255)

                                  aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                              <  1:56740/139‑1 (MQ=255)

                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                           <  8:338682/139‑1 (MQ=255)

                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                           <  5:414688/139‑1 (MQ=255)

                                       ttGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTTCGGCCAGCACAGCCGCCGTTCATTGGTATGTACGCTGAGcc                                                                                         >  1:22755/1‑139 (MQ=255)

                                                         ataAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCCGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                        <  7:375701/138‑1 (MQ=255)

                                                          taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                      <  4:394777/139‑1 (MQ=255)

                                                                  ggcggcTTTCGCTTGCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                              <  6:6162/139‑1 (MQ=255)

                                                                      gctttcgctttcTGCTTTTATTCATCATTCACCGCCATCCGCATTTAAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt                                                          <  2:455046/139‑1 (MQ=255)

                                                                       cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                         <  8:109543/139‑1 (MQ=255)

                                                                       cTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                         <  4:229669/139‑1 (MQ=255)

                                                                            gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                    <  1:38708/139‑1 (MQ=255)

                                                                                    cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                               <  1:83989/135‑1 (MQ=255)

                                                                                                  tCACAGCCCTCCGGCATTCATAAAACGAAACGAGTCCTGCGGGCTGCTCTGCCGCCTTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCTCCCAGTTCATCTATAAgt                               <  7:35917/138‑2 (MQ=255)

                                                                                                      aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATc                          <  5:183214/139‑1 (MQ=255)

                                                                                                        ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                        <  7:219721/139‑1 (MQ=255)

                                                                                                        ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                        <  1:212717/139‑1 (MQ=255)

                                                                                                        acATCCGCAATTCATCACACGAAACGCGGCCTGCGGCCTGCACCGCCGCCTGTCTTTGGGATGTCCGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCAGTCCGGCGTCACCCAGTGCATCAAAAAGACCATCca                        <  4:335224/138‑1 (MQ=255)

                                                                                                          aTCCGCCAGTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                      <  6:38762/139‑1 (MQ=255)

                                                                                                           tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCacac                     <  8:424069/139‑1 (MQ=255)

                                                                                                                cagtCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                <  7:422303/136‑1 (MQ=255)

                                                                                                                      tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa          <  8:454951/139‑1 (MQ=255)

                                                                                                                           cGATACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc     <  3:483331/139‑1 (MQ=255)

                                                                                                                              aaCGAGTCCTGCGGCCTGCACAGCCGCCGGTCTTTGGTATGTTCGCTGAGCCGCATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTGATCAAAAAGACCATCCCCACCCGGAAAGATACAAGCaaa  >  5:218503/1‑139 (MQ=255)

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAA  >  CP006881/3724896‑3725160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A