Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,043 C→A A42A (GCG→GCT)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0430CA100.0% 118.4 / NA 39A42A (GCG→GCT) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/32);  total (7/32)

TCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAA  >  CP006881/3724909‑3725174
                                                                                                                                      |                                                                                                                                   
tcCTGCTTTGGCGATGCCTTGAAGGCTTGCAAACATCTTATTTTATAAAGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                                                   <  4:62862/137‑1 (MQ=255)
  cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                               <  3:318667/139‑2 (MQ=255)
  cTGCTTTGCCTATTCCTTGAAGGCTTGCAACAAGCTTCTTTAATAACCCGCGGCGGCTTTCGCTTTCTGCTTTGCTTCCTCATTCACAGCAAACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                               <  4:273104/139‑2 (MQ=255)
     cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTACGCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgccatt                                                                                                                            >  6:221007/1‑135 (MQ=255)
        tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                                         >  5:118329/1‑139 (MQ=255)
        tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                          <  7:74978/138‑1 (MQ=255)
        tGGCGATGCCTTGACGGCTTGCAAACATCTACTTTTATAAACCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                          <  2:148377/138‑1 (MQ=255)
         ggCGTTGCCTTGAAGGCTTGATAACAGCTTCTTTTTTAAAGCGCGGGGGCTTTCGCTTGCTGCTTTTCTTCCTCATTCACAGCCATCCGCCTTTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  6:252377/139‑1 (MQ=255)
          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCTGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  4:225739/138‑1 (MQ=255)
          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  5:413195/138‑1 (MQ=255)
          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  1:80649/138‑1 (MQ=255)
          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTCTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  4:139964/138‑1 (MQ=255)
              ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                   <  7:413749/139‑1 (MQ=255)
                     aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                            <  3:410875/139‑1 (MQ=255)
                      aGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                            <  7:410671/138‑1 (MQ=255)
                        gCTTGCAAACATCTTCTTTTATATAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  3:124895/139‑1 (MQ=255)
                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  6:118329/139‑1 (MQ=255)
                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  3:339498/139‑1 (MQ=255)
                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  8:210606/139‑1 (MQ=255)
                        gCTTGCAAACATATTATTTTATAATGGGCGGCGGCTTTCGCTTTCTGCTTTTCTACCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  1:358424/139‑1 (MQ=255)
                           tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTAACAGCCATCCGCCATTGATAAAACGAAACGAGTCCTGCCGCCTGCACAGCCGCCATTAATTTGTTAGTTCGCTGAGCCg                                                                                                      >  6:393379/1‑139 (MQ=255)
                             cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                    <  8:312659/139‑1 (MQ=255)
                               aaCATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATa                                                                                                  <  6:221623/139‑1 (MQ=255)
                                             taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                    <  4:178588/139‑1 (MQ=255)
                                                       cggcTTTAGCTTTCTGGTTTTCTTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCGTTAGTTGGTTTGTACGCGGAGCCGAATATACCCCCGCAGCCGCACgttttgt                                                                          >  2:295900/1‑137 (MQ=255)
                                                          cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATGCATAATCCGTAACGAGTCCTGCGGCCTGCACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  7:343054/139‑1 (MQ=255)
                                                          cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAGCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  7:99273/139‑1 (MQ=255)
                                                          cTTTCGCTTTCTGCTTTTCTTCCACATTCACAGCACACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGATTAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  1:207699/139‑1 (MQ=255)
                                                               gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                  <  8:332231/139‑1 (MQ=255)
                                                                       cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTTGTATTTAACCTGAGCCCCATATACCCCGGCCGCCGCCCTTTTTATGTCCGGCGTCACCCAg                                                          >  8:294321/2‑139 (MQ=255)
                                                                                   aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAg                                              <  5:334927/139‑1 (MQ=255)
                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  6:40467/139‑1 (MQ=255)
                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  3:24595/139‑1 (MQ=255)
                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  1:164130/139‑1 (MQ=255)
                                                                                      cacaCCCAGCCGCATTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                                            <  1:285806/138‑1 (MQ=255)
                                                                                         aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTGCCCCAGTTCATAAAAAAGACCCTc                                        >  3:113704/1‑139 (MQ=255)
                                                                                           ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                      <  7:404456/139‑1 (MQ=255)
                                                                                           ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                      <  5:221007/139‑1 (MQ=255)
                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                              <  7:228464/136‑1 (MQ=255)
                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                              <  7:294321/136‑1 (MQ=255)
                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGGATGTACGCTGAGCCGAATAGACCGCGGCCGGCCCAGTTTTTCTGTCCGGCGTCACCCAGTTAATGAAAAAGACCAACCCCACCCGt                               >  2:87557/4‑138 (MQ=255)
                                                                                                     ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTTGTATGTAAGCGGAGCCGAATATACCGCGGCAGCCGCAGTTTTTATTTCCGGGGTCACCCAGTTAATCAAAAAGACCATCCACACCCGTaa                             >  8:2398/2‑138 (MQ=255)
                                                                                                                               ccTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaa  <  1:295900/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAA  >  CP006881/3724909‑3725174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A