Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F57 I19 R4 125 90.3 3545000 95.6% 3389020 127.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,049 T→C K40K (AAA→AAG)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0490TC100.0% 119.9 / NA 33K40K (AAA→AAG) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/29);  total (4/29)

TGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724917‑3725183
                                                                                                                                    |                                                                                                                                       
tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                                    <  4:44082/138‑1 (MQ=255)
 ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  3:32498/139‑1 (MQ=255)
  gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  8:215654/138‑1 (MQ=255)
  gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  7:33067/138‑1 (MQ=255)
  gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  7:33448/138‑1 (MQ=255)
  gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  2:326682/138‑1 (MQ=255)
  gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  3:151781/138‑1 (MQ=255)
            gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                                        <  6:150215/138‑1 (MQ=255)
            gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                                        <  6:157094/138‑1 (MQ=255)
             aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  3:331609/139‑1 (MQ=255)
             aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  2:262593/139‑1 (MQ=255)
             aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  5:109700/139‑1 (MQ=255)
                gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                   <  8:429642/139‑1 (MQ=255)
                     cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                              <  6:36315/139‑1 (MQ=255)
                     cAAAAATCTTCTTTTTTAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATACCCAGCCACCCGCCATACATACACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                              <  6:286639/139‑1 (MQ=255)
                                    ataacccgcgGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  2:263040/132‑1 (MQ=255)
                                    ataAAGCGGGGCGGCTTTCGCTTTCGGCTTTTCTACCTCATTCACAGCCATCCGCCATTAATAAAACGAAACGAGGCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  2:116909/138‑1 (MQ=255)
                                    ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  1:146273/138‑1 (MQ=255)
                                     taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                              <  8:86275/139‑1 (MQ=255)
                                        aGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑g                                                                                          <  3:322458/139‑1 (MQ=255)
                                                  ctttcgctttcgGCTTTTCTTCCTCATTCACCCCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  3:283281/139‑1 (MQ=255)
                                                  cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  4:252576/139‑1 (MQ=255)
                                                  cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  6:103461/139‑1 (MQ=255)
                                                                       ccTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACCGCCGCCTTTCTTTGGTATTTACGCGGATCCCAAATGACCGCGGCACCCGCATGTTTTTAT‑TACGGCGTACACCACTtcatca                                                            >  7:370876/1‑138 (MQ=255)
                                                                            ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                      <  3:147640/139‑1 (MQ=255)
                                                                                   ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  4:426809/139‑1 (MQ=255)
                                                                                   ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  8:171393/139‑1 (MQ=255)
                                                                                     aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                                             <  5:229988/139‑1 (MQ=255)
                                                                                           cattCATACAACGACACGCGTCCTGCGGCCTGCAGCGCCGACTTTCTTTGGTATGTACGCTGAGCCTAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGtt                                       <  7:327411/136‑2 (MQ=255)
                                                                                           cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCTTCCACACCCGTa                                       <  6:178803/136‑1 (MQ=255)
                                                                                           cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                       <  1:322895/136‑1 (MQ=255)
                                                                                                      cGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                            <  1:93790/139‑1 (MQ=255)
                                                                                                        aaaCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCaa                          <  4:176488/139‑1 (MQ=255)
                                                                                                         aaCGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCGGAGCCGAATAGACCGCGGCCGCCGCA‑GGGTTTCTGTGCGGCGTCACCCAGTTCATCAAAAAGCCCAGCCCCACCCCGAAAGATACACGCaaa                         >  3:390946/1‑139 (MQ=255)
                                                                                                              gTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt                    <  5:373851/139‑1 (MQ=255)
                                                                                                                     ggCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa             <  5:187599/139‑1 (MQ=255)
                                                                                                                     ggCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa             <  3:12298/139‑1 (MQ=255)
                                                                                                                         tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCCGCCGCA‑GTTTTTGTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGGAAACATACAAGCAAACGGTTCAAAGaaaaa          >  4:342263/1‑138 (MQ=255)
                                                                                                                                ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTc  >  1:255141/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724917‑3725183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A