Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	113	306.8	9564096	98.5%	9420634	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,118	C→T	intergenic (‑232/‑69)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,118	0	C	T	99.6%	773.2 / NA	225	intergenic (‑232/‑69)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (118/105); total (119/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

ATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGT  >  CP006881/3722973‑3723264

aTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                                                                 >  2:1215024/1‑149 (MQ=255)

aTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                                                                 >  1:3565047/1‑149 (MQ=255)

  tttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTCCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAaattaatt                                                                                                                                               <  1:1258087/150‑1 (MQ=255)

    tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             >  2:29685/1‑150 (MQ=255)

    tAGGTCTTGCCTGCTCTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             <  2:1947911/150‑1 (MQ=255)

        tCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                                                         <  2:716183/150‑1 (MQ=255)

         cTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaat                                                                                                                                        >  1:226402/1‑149 (MQ=255)

           tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                                                                                      <  2:1308538/150‑1 (MQ=255)

           tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTCCATCCTAGATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                                                                                      <  2:3889580/150‑1 (MQ=255)

              cTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATCGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTc                                                                                                                                   <  1:1939870/150‑1 (MQ=255)

               tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                                  >  2:3080878/1‑150 (MQ=255)

                gCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcac                                                                                                                                 >  2:4287975/1‑150 (MQ=255)

                 ccttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGCCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                                                                                <  1:1106963/148‑1 (MQ=255)

                     aCCAGTCACTATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAACTAATTGTGAAATACTTCACAatat                                                                                                                            >  1:411495/1‑150 (MQ=255)

                      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                                                           <  2:4404630/150‑1 (MQ=255)

                        aGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                                                                                                                         <  1:870605/150‑1 (MQ=255)

                        aGTCACAATCCCGCTTATTCAGATCAAGAATACGCTTTCATCATAAATCATGATAGCCTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAACATCGt                                                                                                                         >  1:973969/1‑150 (MQ=255)

                           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAAAATCGTGCt                                                                                                                      >  1:3776722/1‑150 (MQ=255)

                            acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCta                                                                                                                     <  1:4019605/150‑1 (MQ=255)

                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                  <  2:3235975/150‑1 (MQ=255)

                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                  >  2:3594332/1‑150 (MQ=255)

                                tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                                                                 <  1:3867694/150‑1 (MQ=255)

                                   cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                              >  2:1966343/1‑150 (MQ=255)

                                   cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                              >  1:3612270/1‑150 (MQ=255)

                                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaa                                                                                                         >  1:3265658/1‑150 (MQ=255)

                                         ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaat                                                                                                        <  1:1173298/150‑2 (MQ=255)

                                           cAGATTGAGAATACCCTCCCATCATAGATCATGATAGCGTTTCCGTCAACTATTTTTTTAGTTAAATAGTGTGATATCACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCCTGCTATACTATGCTCAATCa                                                                                                      <  2:2133203/150‑1 (MQ=255)

                                            aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                                                     >  1:4420547/1‑150 (MQ=255)

                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtga                                                                                                   <  2:1897107/150‑3 (MQ=255)

                                               ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaa                                                                                                  <  2:1376472/147‑1 (MQ=255)

                                                     aTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                                                            >  2:3723209/1‑150 (MQ=255)

                                                     aTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                                                            >  2:1035598/1‑150 (MQ=255)

                                                      gaCGCTTTCATCATAACTCATGATAGCGTTTTCGACAACTATTTTTTTAGTTAAGTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCCCAACCATGAAAGAAAGc                                                                                           <  2:11575/149‑1 (MQ=255)

                                                       aCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCa                                                                                          <  2:1691681/150‑1 (MQ=255)

                                                         gCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAgg                                                                                        >  2:4549071/1‑150 (MQ=255)

                                                         gCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAgg                                                                                        <  1:4286141/150‑1 (MQ=255)

                                                            ttCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAACTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaa                                                                                     <  2:1677160/150‑1 (MQ=255)

                                                             tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaa                                                                                    <  1:3685308/150‑1 (MQ=255)

                                                               atcatAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGgaaaga                                                                                  >  2:1581946/1‑148 (MQ=255)

                                                                 catAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACa                                                                                <  1:2787190/150‑1 (MQ=255)

                                                                  atAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACaa                                                                               >  2:2051026/1‑150 (MQ=255)

                                                                    aaaTCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                                                             <  1:289156/150‑1 (MQ=255)

                                                                       tcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa                                                                          >  2:3111411/3‑150 (MQ=255)

                                                                          tGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                       >  1:2399731/1‑150 (MQ=255)

                                                                           gATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTc                                                                      <  2:2275955/150‑1 (MQ=255)

                                                                            aTAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                     >  2:4147524/1‑150 (MQ=255)

                                                                            aTAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                     >  2:3163973/1‑150 (MQ=255)

                                                                             tAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCaa                                                                    <  1:3787449/150‑1 (MQ=255)

                                                                              aGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                                                                   >  1:668976/1‑150 (MQ=255)

                                                                                cGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                                 <  1:4042366/150‑1 (MQ=255)

                                                                                cGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                                 <  2:4507812/150‑1 (MQ=255)

                                                                                  ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACATAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt                                                               <  2:927980/150‑1 (MQ=255)

                                                                                  ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt                                                               <  1:1839280/150‑1 (MQ=255)

                                                                                   tttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAGGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtta                                                              >  1:2117635/1‑149 (MQ=255)

                                                                                      cGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaa                                                           <  1:3385650/150‑1 (MQ=255)

                                                                                           actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGt                                                      >  1:2022190/6‑150 (MQ=255)

                                                                                             tatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTCTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGATAAGACAATGGAAACTCAAGCATTACAAAAGGTTg                                                    <  1:4084155/147‑1 (MQ=255)

                                                                                              atttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGa                                                   >  2:1511363/3‑150 (MQ=255)

                                                                                               tttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa                                                  <  1:2884377/149‑1 (MQ=255)

                                                                                                 tttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACa                                                >  2:2162419/1‑150 (MQ=255)

                                                                                                    ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                             <  2:3929900/150‑1 (MQ=255)

                                                                                                      acttAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAGACTATGCTCAATCATGGAAGGAAGCAGGAAAAGGCAATGGAAACTCAAGCATTACAAAAGGCTGAACAGTatg                                           >  2:1865867/3‑148 (MQ=255)

                                                                                                        ttAAATAGTTTGATATACCATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCt                                         <  1:2536660/150‑1 (MQ=255)

                                                                                                         tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCtt                                        >  2:2616247/1‑150 (MQ=255)

                                                                                                           aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTg                                      >  1:159260/1‑150 (MQ=255)

                                                                                                           aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAATATGCTTTg                                      <  2:2585425/150‑1 (MQ=255)

                                                                                                             tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                    <  2:3466414/150‑1 (MQ=255)

                                                                                                               gTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaa                                  <  1:3114614/150‑3 (MQ=255)

                                                                                                               gTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaa                                  <  1:350876/150‑3 (MQ=255)

                                                                                                               gTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGGGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaa                                  >  2:4303828/1‑148 (MQ=255)

                                                                                                                tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGATAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAaaaa                                 <  2:3507151/150‑1 (MQ=255)

                                                                                                                tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAaaaa                                 >  2:4005069/1‑150 (MQ=255)

                                                                                                                tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGGATGCTTTGAaaaa                                 >  1:744750/1‑150 (MQ=255)

                                                                                                                 ttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                <  2:331628/150‑1 (MQ=255)

                                                                                                                  tGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                               <  1:1472469/150‑1 (MQ=255)

                                                                                                                  tGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                               >  1:2568625/1‑150 (MQ=255)

                                                                                                                   gATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                              >  2:2546402/1‑149 (MQ=255)

                                                                                                                    atatAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                             >  2:1008748/1‑148 (MQ=255)

                                                                                                                       taacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAaaaacaaaac                          <  1:1580802/143‑1 (MQ=255)

                                                                                                                         acatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTCTGAAACAACAAAACat                        >  2:4379694/6‑150 (MQ=255)

                                                                                                                           atggaGACAAAAAATCGTAAAAATTAATTGTGAAAAACTTCACAATATCATGCTATACTATGCACAATCATGAAAGAAAGCAGGAAAAAACAATGGGAACTCAAGCATTACAAAGAGTTGAACAGTATGCTTTGAAAAAACAAAACatat                      >  1:1099844/5‑150 (MQ=255)

                                                                                                                            tgtAGACAGAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                     <  1:639882/148‑1 (MQ=255)

                                                                                                                            tgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                     <  1:4549071/148‑1 (MQ=255)

                                                                                                                             gtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttc                    >  2:178639/2‑149 (MQ=255)

                                                                                                                              tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATTTCGTGCTATCCTATGCTCAATCATGAAAGAAAGCAGGAAAAGTCAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTCTGAAAAAACAAAACATAttcg                   <  2:4285135/150‑3 (MQ=255)

                                                                                                                                gACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAGACAAAACATAttcgct                 >  2:507984/1‑146 (MQ=255)

                                                                                                                                gACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgct                 >  1:44698/1‑146 (MQ=255)

                                                                                                                                   aaaaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTca              >  2:340525/1‑149 (MQ=255)

                                                                                                                                   aaaaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTca              >  2:2740164/1‑149 (MQ=255)

                                                                                                                                    aaaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTcaa             >  1:233273/1‑148 (MQ=255)

                                                                                                                                     aaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAg            <  2:3776722/150‑1 (MQ=255)

                                                                                                                                       aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGGAAAAACAAAACATATTCGCTTCAAGCa          >  2:3755392/1‑150 (MQ=255)

                                                                                                                                       aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCa          >  2:163636/1‑150 (MQ=255)

                                                                                                                                         tCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaa        >  2:4357026/1‑150 (MQ=255)

                                                                                                                                          cGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa       >  2:2907881/1‑150 (MQ=255)

                                                                                                                                          cGTAAAAATGAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGGAAAAACAAAACATATTCGGTTCAAGCaaaa       >  2:2494562/1‑150 (MQ=255)

                                                                                                                                             aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc    >  2:2091760/1‑150 (MQ=255)

                                                                                                                                             aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc    >  1:3108480/1‑150 (MQ=255)

                                                                                                                                              aaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCCCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCg   <  1:4636457/150‑1 (MQ=255)

                                                                                                                                               aaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGt  <  1:2964860/150‑1 (MQ=255)

ATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGT  >  CP006881/3722973‑3723264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A