Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F36 I1 R1	5	96.9	4676278	94.6%	4423758	135.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,280	C→T	intergenic (+471/+281)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,280	0	C	T	80.0%	98.3 / 17.1	50	intergenic (+471/+281)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (3/7); new base T (23/17); total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑T‑GCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT  >  NC_002947/4741168‑4741406

ccGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGGACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCgg                                                                                            >  1:1229792/1‑150 (MQ=255)

  gCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGGATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGac                                                                                          >  1:162763/1‑150 (MQ=255)

    ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGacac                                                                                        <  1:1942460/150‑1 (MQ=255)

     tcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACg                                                                                       >  2:303332/1‑150 (MQ=255)

        ttGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcc                                                                                    >  1:1228142/1‑150 (MQ=255)

          gATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcctc                                                                                  >  2:1184915/1‑150 (MQ=255)

           aTGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCTTCAA‑TCCAGGCACAGGCCCCGGACACGtcctcc                                                                                 <  1:2213936/150‑1 (MQ=255)

            tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGTCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCTAC‑TAGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTcctcct                                                                                >  2:1595174/1‑150 (MQ=255)

             ggTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTg                                                                               <  1:1258725/150‑1 (MQ=255)

                cGTAATGTGCAGCACGGAATCACGCGGCTTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACATTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAg                                                                            <  2:943360/150‑1 (MQ=255)

                  tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGa                                                                                           >  1:546306/1‑133 (MQ=255)

                  tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTT‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGa                                                                                           <  2:546306/133‑1 (MQ=255)

                   aaTGTGCAGCAAGGAATCACGCGGCGTCACCGGCCATCGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcgc                                                                         >  1:566422/1‑150 (MQ=255)

                    aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGcc                                                                        <  1:1184915/150‑1 (MQ=255)

                        gcagcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGa                                                                    >  2:2117690/1‑150 (MQ=255)

                             aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                               <  2:2109474/150‑1 (MQ=255)

                                   tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                         >  2:2128997/1‑150 (MQ=255)

                                   tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTT‑GCATTCGTGAATCCAGGC‑ACCGGACGCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                         >  1:1198105/1‑150 (MQ=255)

                                      cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTT‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                      <  1:2117690/150‑1 (MQ=255)

                                           cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                 <  2:2164360/150‑1 (MQ=255)

                                           cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTCAG‑CA‑GC‑TTCTGCGACT‑T‑GCATTCGTGAA‑TGCAGGCACAGGTCCCGGACACGTCCTTCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                 <  2:856465/150‑1 (MQ=255)

                                             tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCGGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                               >  2:778488/1‑150 (MQ=255)

                                              cACCGGCCATGGCTTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAAGCCCCGGACACGTCCTCCTGCATCGCCTCGACATTGGCCCGCGGCGCCTTGAt                                              >  2:396851/1‑150 (MQ=255)

                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                         <  1:1398318/150‑1 (MQ=255)

                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTT‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                         <  2:153522/150‑1 (MQ=255)

                                                    ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                   >  2:2173522/1‑139 (MQ=255)

                                                    ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                   <  1:2173522/139‑1 (MQ=255)

                                                      aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                      <  2:1457056/150‑1 (MQ=255)

                                                         gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGcc                                                                        >  2:1515817/1‑113 (MQ=255)

                                                         gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGcc                                                                        <  1:1515817/113‑1 (MQ=255)

                                                            tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTACTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGGTCACGGTCACACcgc                                >  1:1751817/1‑150 (MQ=255)

                                                            tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TTCGACTT‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGAACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                >  2:2078398/1‑150 (MQ=255)

                                                             aTTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                               >  2:291925/1‑150 (MQ=255)

                                                                 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                           >  1:1675005/1‑150 (MQ=255)

                                                                   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                         <  2:156332/150‑1 (MQ=255)

                                                                   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                         <  1:216686/150‑1 (MQ=255)

                                                                    tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                        >  1:235573/1‑150 (MQ=255)

                                                                             gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                <  2:111767/133‑1 (MQ=255)

                                                                             gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                >  1:111767/1‑133 (MQ=255)

                                                                             gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg               <  2:1229792/150‑1 (MQ=255)

                                                                                cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                 >  1:818151/1‑113 (MQ=255)

                                                                                cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                 <  2:818151/113‑1 (MQ=255)

                                                                                  gCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc          >  2:2332117/1‑150 (MQ=255)

                                                                                  gCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc          <  2:162763/150‑1 (MQ=255)

                                                                                       ggTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa     <  1:347985/150‑1 (MQ=255)

                                                                                        gTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc    >  1:400841/1‑150 (MQ=255)

                                                                                         tCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc   >  1:1478322/1‑150 (MQ=255)

                                                                                          cACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt  <  1:119628/150‑1 (MQ=255)

                                                                                           aCCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTgg                                                              >  2:1268632/1‑89 (MQ=255)

                                                                                           aCCTTCGTGCTTGAG‑CA‑GCTTCT‑GCGACT‑T‑GCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTgg                                                              <  1:1268632/89‑1 (MQ=255)

CCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑T‑GCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT  >  NC_002947/4741168‑4741406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A