Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F19 I1 R1
|
6 |
36.2 |
1751554 |
93.7% |
1641206 |
136.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947 |
4,741,280 |
C→T |
intergenic (+471/+281) |
gltA → / ← yeiW |
citrate synthase/putative oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 4,741,280 | 0 | C | T | 95.2%
| 62.2
/ ‑4.0
| 21 | intergenic (+471/+281) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (11/9); total (11/10) |
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 |
CCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACC‑TCCGCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT > NC_002947/4741171‑4741406
|
cctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGaca > 1:101284/1‑150 (MQ=255)
ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATTGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGtc < 2:354670/150‑1 (MQ=255)
ttGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcc > 1:523953/1‑150 (MQ=255)
aTGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGGATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcctcc > 1:19223/1‑150 (MQ=255)
aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAgcgc < 2:841562/150‑1 (MQ=255)
tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgc > 1:512783/1‑146 (MQ=255)
tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgc < 2:512783/146‑1 (MQ=255)
caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGGCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCtt > 1:675134/1‑150 (MQ=255)
aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg > 1:733404/1‑150 (MQ=255)
cGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc > 2:731377/1‑150 (MQ=255)
ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt < 1:731377/150‑1 (MQ=255)
aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca > 2:710803/1‑150 (MQ=255)
tGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACAGTCACACCGCGAt < 1:28779/150‑1 (MQ=255)
cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc > 1:670309/1‑121 (MQ=255)
cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc < 2:670309/121‑1 (MQ=255)
tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc < 2:19223/150‑1 (MQ=255)
ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa < 2:403309/144‑1 (MQ=255)
ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa > 1:403309/1‑144 (MQ=255)
tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTgg > 1:104497/1‑150 (MQ=255)
cAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc < 2:675134/150‑1 (MQ=255)
cACCTTCGTGCTTGAG‑CAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt < 1:710803/150‑1 (MQ=255)
|
CCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACC‑TCCGCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT > NC_002947/4741171‑4741406
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
