Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F80 I2 R1 7 58.7 2928610 94.0% 2752893 132.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,851,242 G→T E96* (GAG→TAG)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,2420GT100.0% 142.8 / NA 43E96* (GAG→TAG) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/22);  total (21/22)

GTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTGGGCTCGCAGGCGCAGGTA  >  NC_002947/1851095‑1851385
                                                                                                                                                   |                                                                                                                                               
gTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAg                                                                                                                                               <  2:11867/150‑1 (MQ=255)
      aaaaaGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAg                                                                                                                                         >  1:595475/1‑150 (MQ=255)
      aaaaaGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAg                                                                                                                                         <  1:227774/150‑1 (MQ=255)
                 cccGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCgg                                                                                                                              >  1:427696/1‑149 (MQ=255)
                  ccGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGATCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGcc                                                                                                                             >  2:1001570/1‑150 (MQ=255)
                      ccAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAgg                                                                                                                         <  2:367107/150‑1 (MQ=255)
                       cAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGt                                                                                                                        >  2:67465/1‑150 (MQ=255)
                                          ggTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCg                                                                                                     <  1:362952/150‑1 (MQ=255)
                                             aCGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtg                                                                                                  <  1:961398/150‑1 (MQ=255)
                                               gTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCg                                                                                                               >  2:615312/1‑135 (MQ=255)
                                               gTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCg                                                                                                               <  1:615312/135‑1 (MQ=255)
                                               gTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtggt                                                                                                >  1:388136/1‑150 (MQ=255)
                                               gTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtggt                                                                                                <  2:595475/150‑1 (MQ=255)
                                                 ccAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAg                                                                                                                                               <  1:1324192/101‑1 (MQ=255)
                                                  cAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTc                                                                                             >  1:860364/1‑150 (MQ=255)
                                                      ttCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGtt                                                                                                            <  1:816407/131‑1 (MQ=255)
                                                      ttCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGtt                                                                                                            >  2:816407/1‑131 (MQ=255)
                                                                gcCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACg                                                                               <  2:640239/150‑1 (MQ=255)
                                                                   tGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTcagc                                                                                                                    <  2:1076482/110‑1 (MQ=255)
                                                                   tGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTcagc                                                                                                                    >  1:1076482/1‑110 (MQ=255)
                                                                   tGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAg                                                                                         <  2:886197/137‑1 (MQ=255)
                                                                   tGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAg                                                                                         >  1:886197/1‑137 (MQ=255)
                                                                           gCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTgcgc                                                                    >  2:1274988/1‑150 (MQ=255)
                                                                              gAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATg                                                                 >  2:484455/1‑150 (MQ=255)
                                                                                      ggCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCAt                                                         <  2:554611/150‑1 (MQ=255)
                                                                                        cTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCa                                                       <  1:1274988/150‑1 (MQ=255)
                                                                                             cTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGcc                                                  >  2:812761/1‑150 (MQ=255)
                                                                                             cTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGcc                                                  >  2:441551/1‑150 (MQ=255)
                                                                                               cAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGgtg                                                                            >  1:754425/1‑122 (MQ=255)
                                                                                               cAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGgtg                                                                            <  2:754425/122‑1 (MQ=255)
                                                                                                       aCCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGcc                                        <  1:1001570/150‑1 (MQ=255)
                                                                                                              cTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACa                                 >  2:467657/1‑150 (MQ=255)
                                                                                                              cTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACa                                 <  2:860364/150‑1 (MQ=255)
                                                                                                                 ccTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCAt                                                                  <  1:242617/114‑1 (MQ=255)
                                                                                                                 ccTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCAt                                                                  >  2:242617/1‑114 (MQ=255)
                                                                                                                          tGCGTTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCt                     <  2:405437/150‑1 (MQ=255)
                                                                                                                          tGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCt                     >  1:291184/1‑150 (MQ=255)
                                                                                                                             ggTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGTGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTggg                  >  2:135423/1‑150 (MQ=255)
                                                                                                                                      ccGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACg                                                                               >  2:545735/1‑80 (MQ=255)
                                                                                                                                      ccGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACg                                                                               <  1:545735/80‑1 (MQ=255)
                                                                                                                                        gCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGgcc                                                              >  1:1368074/1‑95 (MQ=255)
                                                                                                                                        gCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGgcc                                                              <  2:1368074/95‑1 (MQ=255)
                                                                                                                                             gTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTGGGCTCGCAGGCGCAGGTa  <  1:67465/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTGGGCTCGCAGGCGCAGGTA  >  NC_002947/1851095‑1851385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A